Non-Human Primate CXCL10/IP-10 Cartridge New

Catalog #: SPCKB-NP-011473 Datasheet

Simple Plex Non-Human Primate IP-10 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-NP-011473
SPCKA-NP-011473
SPCKC-NP-011473

Control Products Available

894949: Simple Plex Control for Human CXCL10/IP-10/CRG-2

Non-Human Primate CXCL10/IP-10 Ella Assay Standard Curve

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All CXCL10/IP-10/CRG-2 Simple Plex Assays

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Non-Human Primate CXCL10/IP-10 Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)

Sensitivity

0.186 pg/mL

Assay Range

0.6 - 920 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant IP-10.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of non-human primate Interferon gamma Inducible Protein 10 (IP-10) in serum and plasma (EDTA/Heparin).

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	14	12
Mean (pg/mL)	5.65	283	5.49	297
Standard Deviation	0.347	14.3	0.212	11.7
CV%	6.1	5	3.9	3.9

Recovery

Recovery was not necessary as IP-10 shows 5 points of natural linearity.

Linearity

Samples containing and/or spiked with high concentrations of IP-10 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data

N/A

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Non-Human Primate CXCL10/IP-10 Ella Assay Standard Curve

Product Datasheets

Product Datasheet

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CXCL10/IP-10/CRG-2

IP-10 was originally identified as an IFN-gamma-inducible gene in monocytes, fibroblasts and endothelial cells. The mouse homolog of human IP-10, CRG-2, shares approximately 67% amino acid sequence identity with human IP-10. The amino acid sequence of IP-10 identified the protein as a member of the CXC chemokine subfamily.

Entrez Gene IDs:

3627 (Human); 15945 (Mouse)

Alternate Names:

C7; chemokine (C-X-C motif) ligand 10; CRG2; CRG-2; CXCL10; gIP-10; IFI10; INP10; IP-10; mob-1; SCYB10