Non-Human Primate Angiopoietin like-3 Cartridge

Catalog #: SPCKB-NP-011277 Datasheet
Simple Plex Non-Human Primate ANGPTL3 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer
Catalog # Availability Size / Price Qty
SPCKB-NP-011277
SPCKA-NP-011277
SPCKC-NP-011277
Control Products Available
Non-Human Primate Angiopoietin like-3 Ella Assay Standard Curve
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Non-Human Primate Angiopoietin like-3 Cartridge Summary

Assay Type
Automated sandwich ELISA
Format
Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Assay Length
90 minutes
Sample Type & Volume Required Per Well*
Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity
4.21 pg/mL
Assay Range
15.7 - 24,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant ANGPTL3.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of non-human primate Angiopoietin-like 3 (ANGPTL3) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin).

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 41 42
Mean (pg/mL) 76.4 3522 74.8 3834
Standard Deviation 2.5 186 8.47 459
CV% 3.3 5.3 11.3 12

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=2) 101 100-101

Linearity

Samples containing and/or spiked with high concentrations of ANGPTL3 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Non-Human Primate Angiopoietin like-3 Ella Assay Linearity

Scientific Data

Non-Human Primate Angiopoietin like-3 Ella Assay Standard Curve

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Angiopoietin-like Protein 3/ANGPTL3

Angiopoietin-like, also known as angiopoietin-related proteins, are structurally related to the angiopoietins. They are secreted proteins with N-terminal coiled-coil domains and C-terminal fibrinogen-like domains. Unlike the angiopoietins, ANGPTLs do not bind Tie-2. Seven ANGPTL proteins have been identified (ANGPTL1 through 7).

Entrez Gene IDs:
27329 (Human); 30924 (Mouse); 102136264 (Cynomolgus Monkey)
Alternate Names:
AGNPT5; Ang-5; angiopoietin 5; Angiopoietin-5; angiopoietin-like 3; Angiopoietin-like Protein 3; angiopoietin-related protein 3; ANGPT5angiopoietin-5; ANGPTL3; FHBL2

FAQs

No product specific FAQs exist for this product, however you may

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