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Mouse VEGFR2/KDR/Flk-1 Antibody

Catalog #: MAB443
5 Citations Datasheet
Catalog # Availability Size / Price Qty
MAB443
MAB443-SP
Product Details
Citations (5)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse VEGFR2/KDR/Flk-1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse VEGFR2 in direct ELISAs and Western blots. Does not cross‑react with recombinant mouse (rm) VEGFR1 (Flt-1), recombinant human VEGFR2 (KDR), or rmVEGFR3 (Flt-4).
Source
Monoclonal Rat IgG1 Clone # 91201
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse VEGFR2
Ala20-Glu762
Accession # P35918
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse VEGFR2/KDR/Flk‑1 Fc Chimera (Catalog # 443-KD)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VEGFR2/KDR/Flk-1

VEGFR2 (KDR/Flk-1), VEGFR1 (Flt-1) and VEGFR3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGFR1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis.

Mouse VEGFR2 cDNA encodes a 1367 amino acid (aa) residue precursor protein with a 19 aa residue signal peptide. Mature VEGFR2 is composed of a 743 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 583 aa residue cytoplasmic domain. In contrast to VEGFR1 which binds both PlGF and VEGF with high affinity, VEGFR2 binds VEGF but not PlGF with high affinity. The recombinant soluble VEGFR2/Fc chimera binds VEGF with high affinity and is a potent VEGF antagonist.

References
  1. Ferra, N. and R. Davis-Smyth (1997) Endocrine Reviews 18:4.
  2. Achen, M.G. et al. (1998) Proc. Natl. Acad. Sci. USA. 95:548.
Long Name
Vascular Endothelial Growth Factor Receptor 2
Entrez Gene IDs
3791 (Human); 16542 (Mouse)
Alternate Names
CD309 antigen; CD309; EC 2.7.10; EC 2.7.10.1; Fetal liver kinase 1; fetal liver kinase-1; Flk1; Flk-1; FLK1tyrosine kinase growth factor receptor; KDR; kinase insert domain receptor (a type III receptor tyrosine kinase); Kinase insert domain receptor; KRD1; Ly73; Protein-tyrosine kinase receptor flk-1; soluble VEGFR2; vascular endothelial growth factor receptor 2; VEGF R2; VEGFR; VEGFR2; VEGFR-2

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Citations for Mouse VEGFR2/KDR/Flk-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. 1,25(OH)2D3 regulates the proangiogenic activity of pericyte through VDR-mediated modulation of VEGF production and signaling of VEGF and PDGF receptors
    Authors: Nasim Jamali, Yong‐Seok Song, Christine M. Sorenson, Nader Sheibani
    FASEB BioAdvances
  2. Bim expression modulates the pro-inflammatory phenotype of retinal astroglial cells
    Authors: J Falero-Per, N Sheibani, CM Sorenson
    PLoS ONE, 2020-05-04;15(5):e0232779.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Cyp1b1 expression impacts the angiogenic and inflammatory properties of liver sinusoidal endothelial cells
    Authors: J Falero-Per, YS Song, Y Zhao, L Teixeira, CM Sorenson, N Sheibani
    PLoS ONE, 2018-10-29;13(10):e0206756.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Dysfunctional microvasculature as a consequence of shb gene inactivation causes impaired tumor growth.
    Authors: Funa NS, Kriz V, Zang G, Calounova G, Akerblom B, Mares J, Larsson E, Sun Y, Betsholtz C, Welsh M
    Cancer Res., 2009-02-17;69(5):2141-8.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. PEDF expression affects retinal endothelial cell proangiogenic properties through alterations in cell adhesive mechanisms
    Authors: Juliana Falero-Perez, SunYoung Park, Christine M. Sorenson, Nader Sheibani
    American Journal of Physiology-Cell Physiology

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