Mouse VEGFR1/Flt-1 PE-conjugated Antibody Summary
Ser27-Glu759
Accession # P35969
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of VEGF R1/Flt‑1 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse VEGF R1/Flt-1 PE-conjugated Monoclonal Antibody (Catalog # FAB4711P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Detection of Mouse VEGFR1/Flt-1 by Flow Cytometry Fibroblast-derived VEGF enriches VEGFR1+ cells bone marrow and pre-metastatic sites.(a) Immunohistochemical expression of mouse VEGF in the tumour xenografts established with KYSE150-Id1, KYSE150-Id1-shIGF2 or vector control cells (scale bar, 20 μm). (b) Flow cytometry data showing the expression of VEGFR1+ cells in bone marrow (upper panel) and lungs (lower panel) of mice with subcutaneous implantation of indicated fibroblasts and treatment (female 6–8-week-old nude mice, n=3 per group). (c,d) Flow cytometry analysis of VEGFR1+ cells (c) and comparison of tumour volume (d) of subcutaneous tumour xenografts established from co-implantation of KYSE150 cells and indicated NEFs in the presence or absence of Avastin treatment. Photographs show representative tumours of the three groups (female 6–8-week-old nude mice, n=6 per group; scale bar, 1 cm). Bars, s.d.; *P<0.05; **P<0.01; ***P<0.001 by Student's t-test. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/ncomms14399), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Mouse VEGFR1/Flt-1 by Flow Cytometry Fibroblast-derived VEGF enriches VEGFR1+ cells bone marrow and pre-metastatic sites.(a) Immunohistochemical expression of mouse VEGF in the tumour xenografts established with KYSE150-Id1, KYSE150-Id1-shIGF2 or vector control cells (scale bar, 20 μm). (b) Flow cytometry data showing the expression of VEGFR1+ cells in bone marrow (upper panel) and lungs (lower panel) of mice with subcutaneous implantation of indicated fibroblasts and treatment (female 6–8-week-old nude mice, n=3 per group). (c,d) Flow cytometry analysis of VEGFR1+ cells (c) and comparison of tumour volume (d) of subcutaneous tumour xenografts established from co-implantation of KYSE150 cells and indicated NEFs in the presence or absence of Avastin treatment. Photographs show representative tumours of the three groups (female 6–8-week-old nude mice, n=6 per group; scale bar, 1 cm). Bars, s.d.; *P<0.05; **P<0.01; ***P<0.001 by Student's t-test. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/ncomms14399), licensed under a CC-BY license. Not internally tested by R&D Systems.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: VEGFR1/Flt-1
VEGF R1 is one of the five receptor tyrosine kinases (RTKs) (VEGF R1, KDR/Flk-1, Flt-4, Tie-1, and Tek/Tie-2) whose expression is almost exclusively restricted to the endothelial cells. Tie-1 and tek/tie-2 define a new class of RTKs containing two immunoglobulin-like domains, three EGF homology domains and three fibronectin type III domains in their extracellular regions. VEGF R1/Flt-1, VEGF R2/KDR/Flk-1, VEGF R3/Flt-4 are members of the class III subfamily of RTKs containing seven immunoglobulin-like repeats in their extracellular domains. All five RTKs are likely to play central roles in vasculogenesis and angiogenesis.
Full length mouse VEGF R1 mRNA encodes a 1333 amino acid (aa) residue precursor with a predicted 22 aa residue signal peptide. Mature VEGF R1 is composed of a 737 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 552 aa residue cytoplasmic domain. As a result of alternative splicing of the mRNA, a cDNA encoding a truncated form of VEGF R1, lacking the seventh immunoglobulin-like domain, the transmembrane and intracellular domains, has been cloned. The recombinant soluble VEGF R1/Fc chimera binds VEGF and PlGF with high affinity and is a potent VEGF antagonist.
- He, Y. et al. (1999) Molecular Endocrinology 13:537.
- Ferrara, N. and T. Davis-Smyth (1997) Endocrine Reviews 8:4.
Product Datasheets
Citations for Mouse VEGFR1/Flt-1 PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 8
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Interaction between PD-L1 and soluble VEGFR1 in glioblastoma-educated macrophages
Authors: X Liu, Z Li, J Sun, Z Zhang, W Li
BMC Cancer, 2023-03-20;23(1):259.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Tumor-intrinsic NLRP3-HSP70-TLR4 axis drives premetastatic niche development and hyperprogression during anti-PD-1 immunotherapy
Authors: Theivanthiran B, Yarla N, Haykal T et al.
Science Translational Medicine
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Visualization of Proliferative Vascular Endothelial Cells in Tumors in�Vivo by Imaging Their Partner of Sld5-1 Promoter Activity
Authors: D Yamakawa, W Jia, H Kidoya, S Hosojima, M Torigata, L Zhang, N Takakura
Am. J. Pathol., 2018-04-09;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Flow Cytometry -
Cancer cell-secreted IGF2 instigates fibroblasts and bone marrow-derived vascular progenitor cells to promote cancer progression
Authors: WW Xu, B Li, XY Guan, SK Chung, Y Wang, YL Yip, SY Law, KT Chan, NP Lee, KW Chan, LY Xu, EM Li, SW Tsao, QY He, AL Cheung
Nat Commun, 2017-02-10;8(0):14399.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
VEGFR1-positive macrophages facilitate liver repair and sinusoidal reconstruction after hepatic ischemia/reperfusion injury.
Authors: Ohkubo, Hirotoki, Ito, Yoshiya, Minamino, Tsutomu, Eshima, Koji, Kojo, Ken, Okizaki, Shin-ich, Hirata, Mitsuhir, Shibuya, Masabumi, Watanabe, Masahiko, Majima, Masataka
PLoS ONE, 2014-08-27;9(8):e105533.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Dendritic cells: In vitro culture in two- and three-dimensional collagen systems and expression of collagen receptors in tumors and atherosclerotic microenvironments.
Authors: Sprague L, Muccioli M, Pate M, Singh M, Xiong C, Ostermann A, Niese B, Li Y, Li Y, Courreges M, Benencia F
Exp Cell Res, 2014-02-22;323(1):7-27.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Expression of VEGF receptors on endothelial cells in mouse skeletal muscle.
Authors: Imoukhuede, Princess, Popel, Aleksand
PLoS ONE, 2012-09-12;7(9):e44791.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Bone marrow subsets differentiate into endothelial cells and pericytes contributing to Ewing's tumor vessels.
Authors: Reddy K, Zhou Z, Schadler K
Mol. Cancer Res., 2008-06-01;6(6):929-36.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
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