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Mouse VEGFR1/Flt-1 APC-conjugated Antibody

Catalog #: FAB4711A
6 Citations Datasheet
Catalog # Availability Size / Price Qty
FAB4711A
Detection of VEGF R1/Flt‑1 in bEnd.3 Mouse Cell Line by Flow Cytometry.
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Citations (6)
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Mouse VEGFR1/Flt-1 APC-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse VEGF R1/Flt‑1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human VEGF R1, recombinant mouse (rm) VEGF R2, or rmVEGF R3 is observed.
Source
Monoclonal Rat IgG2B Clone # 141522
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse VEGF R1/Flt‑1
Ser27-Glu759
Accession # P35969
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of VEGF R1/Flt-1 antibody in bEnd.3 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of VEGF R1/Flt‑1 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse VEGF R1/Flt-1 APC-conjugated Monoclonal Antibody (Catalog # FAB4711A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: VEGFR1/Flt-1

VEGF R1 is one of the five receptor tyrosine kinases (RTKs) (VEGF R1, KDR/Flk-1, Flt-4, Tie-1, and Tek/Tie-2) whose expression is almost exclusively restricted to the endothelial cells. Tie-1 and tek/tie-2 define a new class of RTKs containing two immunoglobulin-like domains, three EGF homology domains and three fibronectin type III domains in their extracellular regions. VEGF R1/Flt-1, VEGF R2/KDR/Flk-1, VEGF R3/Flt-4 are members of the class III subfamily of RTKs containing seven immunoglobulin-like repeats in their extracellular domains. All five RTKs are likely to play central roles in vasculogenesis and angiogenesis.

Full length mouse VEGF R1 mRNA encodes a 1333 amino acid (aa) residue precursor with a predicted 22 aa residue signal peptide. Mature VEGF R1 is composed of a 737 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 552 aa residue cytoplasmic domain. As a result of alternative splicing of the mRNA, a cDNA encoding a truncated form of VEGF R1, lacking the seventh immunoglobulin-like domain, the transmembrane and intracellular domains, has been cloned. The recombinant soluble VEGF R1/Fc chimera binds VEGF and PlGF with high affinity and is a potent VEGF antagonist.

References
  1. He, Y. et al. (1999) Molecular Endocrinology 13:537.
  2. Ferrara, N. and T. Davis-Smyth (1997) Endocrine Reviews 8:4.
Long Name
Vascular Endothelial Growth Factor Receptor 1
Entrez Gene IDs
2321 (Human); 14254 (Mouse)
Alternate Names
EC 2.7.10; EC 2.7.10.1; FLT; FLT1; Flt-1; Fms-like tyrosine kinase 1; fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascularpermeability factor receptor); FRT; Tyrosine-protein kinase FRT; Tyrosine-protein kinase receptor FLT; vascular endothelial growth factor receptor 1; Vascular permeability factor receptor; VEGF R1; VEGFR1; VEGFR-1

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Citations for Mouse VEGFR1/Flt-1 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Microglial control of astrocytes in response to microbial metabolites
    Authors: Rothhammer V, Borucki DM, Tjon EC et al.
    Nature
  2. Endothelial cell signature in muscle stem cells validated by VEGFA-FLT1-AKT1 axis promoting survival of muscle stem cell
    Authors: Verma, M;Asakura, Y;Wang, X;Zhou, K;Ünverdi, M;Kann, AP;Krauss, RS;Asakura, A;
    eLife
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Tumor Microenvironment Modulates Immunological Outcomes of Myeloid Cells with mTORC1 Disruption
    Authors: C Ding, X Sun, C Wu, X Hu, HG Zhang, J Yan
    J. Immunol., 2019-01-21;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Antifibrotic role of vascular endothelial growth factor in pulmonary fibrosis
    Authors: LA Murray, DM Habiel, M Hohmann, A Camelo, H Shang, Y Zhou, AL Coelho, X Peng, M Gulati, B Crestani, MA Sleeman, T Mustelin, MW Moore, C Ryu, AD Osafo-Addo, JA Elias, CG Lee, B Hu, JD Herazo-May, DA Knight, CM Hogaboam, EL Herzog
    JCI Insight, 2017-08-17;2(16):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. IL-17-producing gammadelta T cells and neutrophils conspire to promote breast cancer metastasis.
    Authors: Coffelt S, Kersten K, Doornebal C, Weiden J, Vrijland K, Hau C, Verstegen N, Ciampricotti M, Hawinkels L, Jonkers J, de Visser K
    Nature, 2015-03-30;522(7556):345-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Dynamic equilibrium of heterogeneous and interconvertible multipotent hematopoietic cell subsets.
    Authors: Weston W, Zayas J, Perez R, George J, Jurecic R
    Sci Rep, 2014-06-06;4(0):5199.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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