Home / VCAM-1/CD106 / Mouse VCAM‑1/CD106 Biotinylated Antibody

Mouse VCAM‑1/CD106 Biotinylated Antibody

Catalog #: BAF643
6 Citations Datasheet
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BAF643
Product Details
Citations (6)
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Mouse VCAM‑1/CD106 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse VCAM-1/CD106 in Western blots. In this format, approximately 10% cross-reactivity with recombinant human VCAM‑1 under non-reducing conditions is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse VCAM-1/CD106
Phe25-Glu698
Accession # CAA47989
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse VCAM‑1/CD106 Fc Chimera (Catalog # 643-VM)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VCAM-1/CD106

VCAM-1 (CD106), a member of the immunoglobulin superfamily, is a cell surface protein expressed by activated endothelial cells and certain leukocytes (such as macrophages). VCAM-1 expression is induced by IL-1 beta, IL-4, TNF-alpha, and IFN-gamma. VCAM-1 binds to leukocyte integrins VLA-4 and alpha 4 beta 7. The human and mouse VCAM-1 proteins share approximately 76% amino acid similarity.

During the inflammatory adhesion mechanism, activated integrins halt rolling leukocytes and attach them firmly to the vascular endothelium. They do this by binding to their ligands, for example VCAM-1, on endothelium. The VCAM-1: VLA-4/ alpha 4 beta 7 interaction is also thought to be involved in the extravasation of white blood cells through the blood vessel wall to sites of inflammation.

ELISA techniques have shown that detectable levels of soluble VCAM-1 are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of VCAM-1 may be elevated or lowered in subjects with a variety of pathological conditions.

Long Name
Vascular Cell Adhesion Molecule 1
Entrez Gene IDs
7412 (Human); 22329 (Mouse)
Alternate Names
CD106 antigen; CD106; DKFZp779G2333; INCAM-100; L1CAM; MGC99561; vascular cell adhesion molecule 1; vascular cell adhesion protein 1; V-CAM 1; VCAM1; VCAM-1

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Citations for Mouse VCAM‑1/CD106 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Essential Role of Canonical NF-kappa B Activity in the Development of Stromal Cell Subsets in Secondary Lymphoid Organs
    Authors: Dana Bogdanova, Arata Takeuchi, Madoka Ozawa, Yasuhiro Kanda, M. Azizur Rahman, Burkhard Ludewig et al.
    The Journal of Immunology
  2. A Distinct Subset of Fibroblastic Stromal Cells Constitutes the Cortex-Medulla Boundary Subcompartment of the Lymph Node
    Authors: Arata Takeuchi, Madoka Ozawa, Yasuhiro Kanda, Mina Kozai, Izumi Ohigashi, Yoichi Kurosawa et al.
    Frontiers in Immunology
  3. Autotaxin produced by stromal cells promotes LFA-1-independent and Rho-dependent interstitial T cell motility in the lymph node paracortex.
    Authors: Katakai T, Kondo N, Ueda Y, Kinashi T
    J Immunol, 2014-06-16;193(2):617-26.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Organizer-like reticular stromal cell layer common to adult secondary lymphoid organs.
    Authors: Katakai T, Suto H, Sugai M, Gonda H, Togawa A, Suematsu S, Ebisuno Y, Katagiri K, Kinashi T, Shimizu A
    J. Immunol., 2008-11-01;181(9):6189-200.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Elevated urinary VCAM-1, P-selectin, soluble TNF receptor-1, and CXC chemokine ligand 16 in multiple murine lupus strains and human lupus nephritis.
    Authors: Wu T, Xie C, Wang HW, Zhou XJ, Schwartz N, Calixto S, Mackay M, Aranow C, Putterman C, Mohan C
    J. Immunol., 2007-11-15;179(10):7166-75.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Excreted urinary mediators in an animal model of experimental immune nephritis with potential pathogenic significance.
    Authors: Wu T, Xie C, Bhaskarabhatla M, Yan M, Leone A, Chen SS, Zhou XJ, Putterman C, Mohan C
    Arthritis Rheum., 2007-03-01;56(3):949-59.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC

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