Mouse VCAM-1/CD106 Antibody Summary
Phe25-Glu698
Accession # P29533
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Mouse VCAM‑1/CD106 by Western Blot. Western blot shows lysates of 3T3-L1 mouse embryonic fibroblast adipose-like cell line, C2C12 mouse myoblast cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse VCAM-1/CD106 Monoclonal Antibody (Catalog # MAB6434) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for VCAM-1/ CD106 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Mouse VCAM‑1/CD106 by Simple WesternTM. Simple Western lane view shows lysate of 3T3-L1 mouse embryonic fibroblast adipose-like cell line, loaded at 0.2 mg/mL. A specific band was detected for VCAM-1/CD106 at approximately 117 kDa (as indicated) using 100 µg/mL of Rat Anti-Mouse VCAM-1/CD106 Monoclonal Antibody (Catalog # MAB6434) followed by 1:50 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Detection of Mouse VCAM-1/CD106 by Western Blot Effect of statin treatment on aortic expression of VCAM-1.(A) VCAM-1 protein expression in the ascending aorta assessed by Western blot in non-treated (lanes 1–5) versus statin treated (lanes 6–10) animals, n = 5 per group, *p<0.01 vs non-treated animals. Representative examples of fluorescent immunohistochemistry images of the base of the aorta demonstrating endothelial VCAM-1 expression (red fluorescence) in a non-treated animal (B) and a statin treated animal (C), the green fluorescence is autofluorescence delineating vessel anatomy. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/23554922), licensed under a CC-BY license. Not internally tested by R&D Systems.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VCAM-1/CD106
VCAM-1 (CD106), a member of the immunoglobulin superfamily, is a cell surface protein expressed by activated endothelial cells and certain leukocytes (such as macrophages). VCAM-1 expression is induced by IL-1 beta, IL-4, TNF-alpha, and IFN-gamma. VCAM-1 binds to leukocyte integrins VLA-4 and alpha 4 beta 7. The human and mouse VCAM-1 proteins share approximately 76% amino acid similarity.
During the inflammatory adhesion mechanism, activated integrins halt rolling leukocytes and attach them firmly to the vascular endothelium. They do this by binding to their ligands, for example VCAM-1, on endothelium. The VCAM-1: VLA-4/ alpha 4 beta 7 interaction is also thought to be involved in the extravasation of white blood cells through the blood vessel wall to sites of inflammation.
ELISA techniques have shown that detectable levels of soluble VCAM-1 are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of VCAM-1 may be elevated or lowered in subjects with a variety of pathological conditions.
Product Datasheets
Citations for Mouse VCAM-1/CD106 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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Plasma APE1/Ref-1 Correlates with Atherosclerotic Inflammation in ApoE-/- Mice
Authors: YR Lee, HK Joo, EO Lee, MS Park, HS Cho, S Kim, H Jin, JO Jeong, CS Kim, BH Jeon
Biomedicines, 2020-09-21;8(9):.
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ADAM10 facilitates rapid neural stem cell cycling and proper positioning within the subventricular zone niche via JAMC/RAP1Gap signaling
Authors: N McMillan, GW Kirschen, S Desai, E Xia, SE Tsirka, A Aguirre
Neural regeneration research, 2022-11-01;17(11):2472-2483.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
LPS, but not Angiotensin ll, lnduces Direct Pro-lnflammatory Effects in Cultured Mouse Arteries and Human Endothelial and Vascular Smooth Muscle Cells
Authors: Majid Sheykhzade
Basic Clin. Pharmacol. Toxicol., 2017-01-06;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Noninvasive ultrasound molecular imaging of the effect of statins on endothelial inflammatory phenotype in early atherosclerosis.
Authors: Khanicheh E, Mitterhuber M, Xu L, Haeuselmann S, Kuster G, Kaufmann B
PLoS ONE, 2013-03-15;8(3):e58761.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
FAK inhibition reduces metastasis of alpha 4 integrin-expressing melanoma to lymph nodes by targeting lymphatic VCAM-1 expression
Authors: Kyuho Jeong, James M. Murphy, Yelitza A.R. Rodriguez, Jun-Sub Kim, Eun-Young Erin Ahn, Ssang-Taek Steve Lim
Biochemical and Biophysical Research Communications
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