Mouse Tyro3/Dtk Biotinylated Antibody

Catalog #: BAM759
1 Citation 1 Review Datasheet

Catalog #	Availability	Size / Price	Qty
BAM759

All Tyro3/Dtk Antibodies

Product Details

Citations (1)

FAQs

Supplemental Products

Reviews (1)

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Tyro3/Dtk Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Recognizes the extracellular domain of mouse Tyro3/Dtk. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Tyro3/Dtk, recombinant mouse (rm) Mer, or rmAxl is observed.

Source

Monoclonal Rat IgG₁ Clone # 109646

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Tyro3/Dtk
Met1-Ser418
Accession # P55144

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse Tyro3/Dtk Fc Chimera (Catalog # 759-DT)

Immunohistochemistry

8-25 µg/mL

Immersion fixed frozen sections of mouse embryo (E15)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tyro3/Dtk

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt) and Mer (human and mouse homologues of chicken c-Eyk) constitute a new receptor tyrosine kinase subfamily. The extracellular domain of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. All three receptors bind the vitamin K-dependent protein growth-arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. The binding affinities for Gas6 is in the order of Axl > Dtk > Mer. Gas6 binding induces tyrosine phosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis. Dtk is widely expressed during embryonic development. In adults, Dtk is predominantly expressed in neurons in restricted regions of the brain.

References

Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.

Long Name

Tyrosine Protein Kinase Receptor TYRO3

Entrez Gene IDs

7301 (Human); 22174 (Mouse)

Alternate Names

Brt; BYK; Dtk; EC 2.7.10; EC 2.7.10.1; FLJ16467; Rse; Sky; Tif; TYRO3 protein tyrosine kinase; Tyro3; Tyrosine-protein kinase byk; Tyrosine-protein kinase DTK; tyrosine-protein kinase receptor TYRO3; Tyrosine-protein kinase RSE; Tyrosine-protein kinase SKY

Product Datasheets

Product Datasheet

Related Research Areas

Citation for Mouse Tyro3/Dtk Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Harnessing MerTK agonism for targeted therapeutics
Authors: Vivekananda Kedage, Diego Ellerman, Yongmei Chen, Wei-Ching Liang, Joven Borneo, Yan Wu et al.
mAbs