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Mouse Tyro3/Dtk Antibody

Catalog #: AF759
13 Citations Datasheet
Catalog # Availability Size / Price Qty
AF759
AF759-SP
Tyro3/Dtk in Mouse Embryo.
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Citations (13)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Tyro3/Dtk Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Tyro3/Dtk in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Tyro3/Dtk
Ala31-Ser418
Accession # P55144
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Tyro3/Dtk Fc Chimera (Catalog # 759-DT)
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Tyro3/Dtk antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

Tyro3/Dtk in Mouse Embryo. Tyro3/Dtk was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c, follicles of vibrissae associated with upper lip) using Goat Anti-Mouse Tyro3/Dtk Antigen Affinity-purified Polyclonal Antibody (Catalog # AF759) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counter-stained with hematoxylin (blue). Specific labeling was localized to the follicles of vibrissa. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tyro3/Dtk

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt) and Mer (human and mouse homologues of chicken c-Eyk) constitute a new receptor tyrosine kinase subfamily. The extracellular domain of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. All three receptors bind the vitamin K-dependent protein growth-arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. The binding affinities for Gas6 is in the order of Axl > Dtk > Mer. Gas6 binding induces tyrosine phosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis. Dtk is widely expressed during embryonic development. In adults, Dtk is predominantly expressed in neurons in restricted regions of the brain.

References
  1. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
  2. Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.
Long Name
Tyrosine Protein Kinase Receptor TYRO3
Entrez Gene IDs
7301 (Human); 22174 (Mouse)
Alternate Names
Brt; BYK; Dtk; EC 2.7.10; EC 2.7.10.1; FLJ16467; Rse; Sky; Tif; TYRO3 protein tyrosine kinase; Tyro3; Tyrosine-protein kinase byk; Tyrosine-protein kinase DTK; tyrosine-protein kinase receptor TYRO3; Tyrosine-protein kinase RSE; Tyrosine-protein kinase SKY

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Citations for Mouse Tyro3/Dtk Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Anti-inflammatory clearance of amyloid-beta by a chimeric Gas6 fusion protein
    Authors: H Jung, SY Lee, S Lim, HR Choi, Y Choi, M Kim, S Kim, Y Lee, KH Han, WS Chung, CH Kim
    Nature Medicine, 2022-08-04;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. IL-4/IL-13 polarization of macrophages enhances Ebola virus glycoprotein-dependent infection
    Authors: KJ Rogers, B Brunton, L Mallinger, D Bohan, KM Sevcik, J Chen, N Ruggio, W Maury
    PLoS Negl Trop Dis, 2019-12-11;13(12):e0007819.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Sensing of apoptotic cells through Axl causes lung basal cell proliferation in inflammatory diseases
    Authors: Naoya Fujino, Oliver J. Brand, David J. Morgan, Toshifumi Fujimori, Aleksander M. Grabiec, Christopher P. Jagger et al.
    Journal of Experimental Medicine
  4. Gas6/TAM Receptors in Systemic Lupus Erythematosus
    Authors: Philip L. Cohen, Wen-Hai Shao
    Disease Markers
  5. Mouse macrophages show different requirements for phosphatidylserine receptor Tim4 in efferocytosis
    Authors: Y Yanagihash, K Segawa, R Maeda, YI Nabeshima, S Nagata
    Proc. Natl. Acad. Sci. U.S.A., 2017-08-02;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Migrating Interneurons Secrete Fractalkine to Promote Oligodendrocyte Formation in the Developing Mammalian Brain
    Authors: A Voronova, SA Yuzwa, BS Wang, S Zahr, C Syal, J Wang, DR Kaplan, FD Miller
    Neuron, 2017-05-03;94(3):500-516.e9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  7. Diversification of TAM receptor tyrosine kinase function.
    Authors: Zagorska A, Traves P, Lew E, Dransfield I, Lemke G
    Nat Immunol, 2014-09-07;15(10):920-8.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  8. The E3 ligase Cbl-b and TAM receptors regulate cancer metastasis via natural killer cells.
    Authors: Paolino, Magdalen, Choidas, Axel, Wallner, Stephani, Pranjic, Blanka, Uribesalgo, Iris, Loeser, Stefanie, Jamieson, Amanda M, Langdon, Wallace, Ikeda, Fumiyo, Fededa, Juan Pab, Cronin, Shane J, Nitsch, Roberto, Schultz-Fademrecht, Carsten, Eickhoff, Jan, Menninger, Sascha, Unger, Anke, Torka, Robert, Gruber, Thomas, Hinterleitner, Reinhard, Baier, Gottfrie, Wolf, Dominik, Ullrich, Axel, Klebl, Bert M, Penninger, Josef M
    Nature, 2014-02-19;507(7493):508-12.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. The TAM-family receptor Mer mediates production of HGF through the RhoA-dependent pathway in response to apoptotic cells
    Authors: Hyun-Jung Park, Ji-Yeon Baen, Ye-Ji Lee, Youn-Hee Choi, Jihee Lee Kang
    Molecular Biology of the Cell
  10. Protein S protects neurons from excitotoxic injury by activating the TAM receptor Tyro3-phosphatidylinositol 3-kinase-Akt pathway through its sex hormone-binding globulin-like region.
    Authors: Zhong Z, Wang Y, Guo H, Sagare A, Fernandez JA, Bell RD, Barrett TM, Griffin JH, Freeman RS, Zlokovic BV
    J. Neurosci., 2010-11-17;30(46):15521-34.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  11. Protein S controls hypoxic/ischemic blood-brain barrier disruption through the TAM receptor Tyro3 and sphingosine 1-phosphate receptor.
    Authors: Zhu D, Wang Y, Singh I
    Blood, 2010-03-26;115(23):4963-72.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  12. Gas6 receptors Axl, Sky and Mer enhance platelet activation and regulate thrombotic responses.
    Authors: Gould WR, Baxi SM, Schroeder R, Peng YW, Leadley RJ, Peterson JT, Perrin LA
    J. Thromb. Haemost., 2005-02-23;3(4):733-41.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Differential TAM receptor-ligand-phospholipid interactions delimit differential TAM bioactivities.
    Authors: Lew ED, Oh J, Burrola PG et al.
    Elife.

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