Mouse Tyro3/Dtk Antibody Summary
Ala31-Ser418
Accession # P55144
Applications
Mouse Tyro3/Dtk Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Tyro3/Dtk in Transfected HEK 293 (positive) and mIMCD-3 (positive). Tyro3/Dtk was detected in immersion fixed Transfected HEK293 human embryonic kidney cells (positive) and mIMCD‑3 mouse epithelial cell line (positive) using Rat Anti-Mouse Tyro3/Dtk Monoclonal Antibody (Catalog # MAB759) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Detection of Tyro3/Dtk in Transfected HEK 293 (positive) and wild-type HEK 293 (negative) cells. Tyro3/Dtk was detected in immersion fixed Transfected HEK293 human embryonic kidney cells (positive) and absent in wild-type HEK293 human embryonic kidney cells (negative) using Rat Anti-Mouse Tyro3/Dtk Monoclonal Antibody (Catalog # MAB759) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Tyro3/Dtk
Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt) and Mer (human and mouse homologues of chicken c-Eyk) constitute a new receptor tyrosine kinase subfamily. The extracellular domain of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. All three receptors bind the vitamin K-dependent protein growth-arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. The binding affinities for Gas6 is in the order of Axl > Dtk > Mer. Gas6 binding induces tyrosine phosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis. Dtk is widely expressed during embryonic development. In adults, Dtk is predominantly expressed in neurons in restricted regions of the brain.
- Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
- Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.
Product Datasheets
Citations for Mouse Tyro3/Dtk Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
8
Citations: Showing 1 - 8
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Development of a Spontaneous Liver Disease Resembling Autoimmune Hepatitis in Mice Lacking Tyro3, Axl and Mer Receptor Tyrosine Kinases
Authors: Nan Qi, Peipei Liu, Yue Zhang, Hui Wu, Yongmei Chen, Daishu Han
PLoS ONE
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MerTK Induces Dysfunctional Dendritic Cells by Metabolic Reprogramming
Authors: Zewdie, EY;Edwards, GM;Hunter, DM;Earp, HS;Holtzhausen, A;
Cancer immunology research
Species: Mouse
Sample Types: Whole Cells
Applications: Mass Spectrometry -
Gas6 Attenuates Sepsis-Induced Tight Junction Injury and Vascular Endothelial Hyperpermeability via the Axl/NF-kappaB Signaling Pathway
Authors: J Ni, M Lin, Y Jin, J Li, Y Guo, J Zhou, G Hong, G Zhao, Z Lu
Front Pharmacol, 2019-06-13;10(0):662.
Species: Mouse, Rat
Sample Types: Cell Lysates
Applications: Western Blot -
Pan-TAM tyrosine kinase inhibitor BMS-777607 enhances anti-PD-1 mAb efficacy in a murine model of triple-negative breast cancer
Authors: RB Birge, C Kasikara, V Davra, D Calianese, K Geng, TE Spires, M Quigley, M Wichroski, G Sriram, L Suarez-Lop, MB Yaffe, SV Kotenko, MS De Lorenzo
Cancer Res., 2019-03-15;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
The Axl receptor tyrosine kinase is a discriminator of macrophage function in the inflamed lung.
Authors: Fujimori T, Grabiec A, Kaur M, Bell T, Fujino N, Cook P, Svedberg F, MacDonald A, Maciewicz R, Singh D, Hussell T
Mucosal Immunol, 2015-01-21;8(5):1021-30.
Species: Mouse
Sample Types: BALF
Applications: Flow Cytometry -
GAS6 receptor status is associated with dormancy and bone metastatic tumor formation.
Authors: Taichman, Russell, Patel, Lalit R, Bedenis, Rachel, Wang, Jingchen, Weidner, Savannah, Schumann, Taibrian, Yumoto, Kenji, Berry, Janice E, Shiozawa, Yusuke, Pienta, Kenneth
PLoS ONE, 2013-04-24;8(4):e61873.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Toll-like receptor-mediated inhibition of Gas6 and ProS expression facilitates inflammatory cytokine production in mouse macrophages.
Authors: Deng T, Zhang Y, Chen Q, Yan K, Han D
Immunology, 2012-01-01;135(1):40-50.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Toll-like receptor-initiated testicular innate immune responses in mouse Leydig cells.
Authors: Shang T, Zhang X, Wang T, Sun B, Deng T, Han D
Endocrinology, 2011-05-03;152(7):2827-36.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
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