Mouse Trypsin 3/PRSS3 Alexa Fluor® 700-conjugated Antibody

Catalog #: FAB35651N Datasheet
Catalog # Availability Size / Price Qty
FAB35651N-100UG
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Mouse Trypsin 3/PRSS3 Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Trypsin 3/PRSS3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, weak cross-reactivity with recombinant human (rh) Trypsin 3 is observed and no cross-reactivity with rhTrypsin 1 or rhTrypsin 2 is ob
Source
Monoclonal Rat IgG2b Clone # 419910
Purification
Protein A or G purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Trypsin 3/PRSS3
Phe16-Asn246
Accession # NP_035775
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration
Sample
Western Blot
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Trypsin 3/PRSS3

Trypsin 3, encoded by the PRSS3 gene and also known as mesotrypsin is a serine protease and is one of three pancreatic Trypsins. It constitutes less than 10% of the total Trypsin content in normal pancreatic juice. Trypsin 3 is activated by enterokinase cleavage of the 8 aa pro region and secreted into the duodenum lumen. Trypsin 3 is highly resistance to polypeptide Trypsin inhibitors, and may degrade them in foods to facilitate digestion. The 223 amino acid mature mouse Trypsin 3 shares 78% and 96% aa identity with human and rat Trypsin 3, respectively.

Entrez Gene IDs
5646 (Human); 22073 (Mouse); 362347 (Rat)
Alternate Names
Brain trypsinogen; EC 3.4.21; EC 3.4.21.4; Mesotrypsin; mesotrypsinogen; MTG; pancreatic trypsinogen III; protease, serine, 3 (mesotrypsin); protease, serine, 3; protease, serine, 4 (trypsin 4, brain); PRSS3; PRSS4; Serine protease 3; Serine protease 4; T9; TRY3MTG; TRY4mesotrypsin; Trypsin 3; Trypsin 4; Trypsin III; Trypsin IV; trypsin-3; Trypsinogen 15; trypsinogen 4; trypsinogen 5; trypsinogen IV

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