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Mouse TREM-1 Antibody

Catalog #: MAB1187
15 Citations 1 Review Datasheet
Catalog # Availability Size / Price Qty
MAB1187-SP
MAB1187-100
MAB1187-500
Detection of TREM‑1 in Mouse Splenocytes by Flow Cytometry.
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Citations (15)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse TREM-1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse TREM-1 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse TREM-2B, recombinant human (rh) TREM-1, or rhTREM-2 is observed.
Source
Monoclonal Rat IgG2A Clone # 174031
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TREM-1
Ala21-Ser202
Accession # Q9JKE2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse TREM‑1 Fc Chimera (Catalog # 1187-TR)
Flow Cytometry
0.25 µg/106 cells
See below
Agonist Activity
Measured by its ability to stimulate TNF-alpha secretion by P388D1 mouse lymphoma cells (ATCC # TIB-63). Bouchon, A. et al. (2001) Nature, 410:1103 and Bouchon, A. et al. (2000) J. Immunology, 164:4991.
The ED50 for this effect is typically 0.4-3 μg/mL.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of TREM-1 antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of TREM‑1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse Gr-1/Ly-6G Alexa Fluor® 405-conjugated Monoclonal Antibody (Catalog # FAB1037V) and either (A) Rat Anti-Mouse TREM-1 Monoclonal Antibody (Catalog # MAB1187) or (B) Rat IgG2AIsotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).

Immunocytochemistry TREM‑1 antibody in Mouse Splenocytes by Immunocytochemistry (ICC). View Larger

TREM‑1 in Mouse Splenocytes. TREM-1 was detected in immersion fixed mouse splenocytes treated with LPS using Rat Anti-Mouse TREM-1 Monoclonal Antibody (Catalog # MAB1187) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TREM-1

TREM-1 (Triggering Receptor Expressed on Myeloid cells) is a type I transmembrane protein having a single Ig-like domain. It associates with the adapter protein, DAP12, to deliver an activating signal. Several other TREM family members have been reported that are structurally similar but share less than 30% amino acid identity. TREM-1 is expressed on blood neutrophils and a subset of monocytes, and expression is up-regulated by bacterial LPS. Engagement of TREM-1 with a monoclonal antibody leads to expression of IL-8, MCP-1, and TNF-alpha suggesting that this receptor plays an important role in inflammatory responses. TREM-1 is expressed at high levels on neutrophils of patients with microbial sepsis and in mice with LPS-induced shock. Blockade of TREM-1 with a TREM-1/Fc fusion protein protected mice against LPS-induced shock.

References
  1. Bouchon, A. (2000) J. Immunol. 164:4991.
  2. Bouchon, A. (2001) Nature 410:1103.
  3. Nathan, C. and A. Ding (2001) Nature Med. 7:530.
Long Name
Triggering Receptor Expressed on Myeloid Cells 1
Entrez Gene IDs
54210 (Human); 58217 (Mouse)
Alternate Names
CD354 antigen; CD354; TREM1; TREM-1; TREM-1Triggering receptor expressed on monocytes 1; triggering receptor expressed on myeloid cells 1; triggering-receptor TREM1

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Citations for Mouse TREM-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. FOXM1 network in association with TREM1 suppression regulates NET formation in diabetic foot ulcers
    Authors: Sawaya AP, Stone RC, Mehdizadeh S et al.
    EMBO reports
  2. Inhibition of Lung Tumor Development in ApoE Knockout Mice via Enhancement of TREM-1 Dependent NK Cell Cytotoxicity
    Authors: Yong Sun Lee, In Jun Yeo, Ki Cheon Kim, Sang-Bae Han, Jin Tae Hong
    Frontiers in Immunology
  3. Triggering Receptor Expressed on Myeloid Cells-1 Agonist Regulates Intestinal Inflammation via Cd177+ Neutrophils
    Authors: Dong Hyuk Seo, Xiumei Che, Soochan Kim, Da Hye Kim, Hyun Woo Ma, Jae Hyeon Kim et al.
    Frontiers in Immunology
  4. Impaired lipophagy induced-microglial lipid droplets accumulation contributes to the buildup of TREM1 in diabetes-associated cognitive impairment
    Authors: Li, Q;Zhao, Y;Guo, H;Li, Q;Yan, C;Li, Y;He, S;Wang, N;Wang, Q;
    Autophagy
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. TREM-1 triggers necroptosis of macrophages through mTOR-dependent mitochondrial fission during acute lung injury
    Authors: WJ Zhong, J Zhang, JX Duan, CY Zhang, SC Ma, YS Li, NS Yang, HH Yang, JB Xiong, CX Guan, ZX Jiang, ZJ You, Y Zhou
    Journal of Translational Medicine, 2023-03-06;21(1):179.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ELISA Capture
  6. Exosomes containing HIV protein Nef reorganize lipid rafts potentiating inflammatory response in bystander cells
    Authors: N Mukhamedov, A Hoang, D Dragoljevi, L Dubrovsky, T Pushkarsky, H Low, M Ditiatkovs, Y Fu, R Ohkawa, PJ Meikle, A Horvath, B Brichacek, YI Miller, A Murphy, M Bukrinsky, D Sviridov
    PLoS Pathog., 2019-07-25;15(7):e1007907.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  7. TREM-1 regulates neutrophil chemotaxis by promoting NOX-dependent superoxide production
    Authors: S Baruah, S Murthy, K Keck, I Galvan, A Prichard, LH Allen, M Farrelly, J Klesney-Ta
    J. Leukoc. Biol., 2019-01-22;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. HIV-related proteins prolong macrophage survival through induction of Triggering receptor expressed on myeloid cells-1
    Authors: Z Yuan, X Fan, B Staitieh, C Bedi, P Spearman, DM Guidot, RT Sadikot
    Sci Rep, 2017-02-09;7(0):42028.
  9. TREM-2 promotes macrophage survival and lung disease after respiratory viral infection.
    Authors: Wu K, Byers D, Jin X, Agapov E, Alexander-Brett J, Patel A, Cella M, Gilfilan S, Colonna M, Kober D, Brett T, Holtzman M
    J Exp Med, 2015-04-20;212(5):681-97.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Flow Cytometry
  10. TREM-1 deficiency can attenuate disease severity without affecting pathogen clearance.
    Authors: Weber B, Schuster S, Zysset D, Rihs S, Dickgreber N, Schurch C, Riether C, Siegrist M, Schneider C, Pawelski H, Gurzeler U, Ziltener P, Genitsch V, Tacchini-Cottier F, Ochsenbein A, Hofstetter W, Kopf M, Kaufmann T, Oxenius A, Reith W, Saurer L, Mueller C
    PLoS Pathog, 2014-01-16;10(1):e1003900.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay
  11. Comparison of gene expression profiles between human and mouse monocyte subsets.
    Authors: Ingersoll MA, Spanbroek R, Lottaz C
    Blood, 2009-11-12;115(3):e10-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. TREM-1 activation alters the dynamics of pulmonary IRAK-M expression in vivo and improves host defense during pneumococcal pneumonia.
    Authors: Lagler H, Sharif O, Haslinger I, Matt U, Stich K, Furtner T, Doninger B, Schmid K, Gattringer R, de Vos AF, Knapp S
    J. Immunol., 2009-07-13;183(3):2027-36.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  13. Salmonella effector SpvB aggravates dysregulation of systemic iron metabolism via modulating the hepcidin-ferroportin axis
    Authors: Deng Q, Yang S, Sun L, et al.
    Gut microbes
  14. TREM-1 orchestrates angiotensin II–induced monocyte trafficking and promotes experimental abdominal aortic aneurysm
    Authors: Marie Vandestienne, Yujiao Zhang, Icia Santos-Zas, Rida Al-Rifai, Jeremie Joffre, Andreas Giraud et al.
    Journal of Clinical Investigation
  15. TREM-1 governs NLRP3 inflammasome activation of macrophages by firing up glycolysis in acute lung injury
    Authors: Zhong WJ, Liu T, Yang HH et al.
    International journal of biological sciences

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Mouse TREM-1 Antibody
By Anonymous on 08/28/2022
Application: Flow Sample Tested: Spleen tissue Species: Mouse
Flow Cytometry Mouse TREM-1 Antibody MAB1187