Mouse TIM-3 PE-conjugated Antibody Summary
Arg20-Ala193
Accession # Q8VIM0
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of TIM‑3 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse TIM-3 PE-conjugated Monoclonal Antibody (Catalog # FAB1529P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: TIM-3
TIM-3 (T cell immunoglobulin and mucin domain-3) is a 60 kDa member of the TIM family of immune regulating molecules. TIMs are type I transmembrane glycoproteins with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk (1 - 3). There are three TIM genes in human and eight in mouse. Mature mouse TIM-3 consists of a 174 aa extracellular domain (ECD), a 21 aa transmembrane segment (TM), and a 67 aa cytoplasmic tail (4). Two alternately spliced isoforms have been reported in mouse which lack either the TM or both the TM and mucin regions (5, 6). Within the ECD, mouse TIM-3 shares 58% and 74% aa sequence identity with human and rat TIM-3, respectively. TIM-3 is specifically expressed on Th1 cells whereas TIM-1 and TIM-2 are expressed on TH2 cells. In chronic inflammation, autoimmune disorders, and some cancers, TIM-3 is upregulated on several other hematopoietic cell types and on hippocampal neurons (9 - 12). The glycosylated Ig domain of TIM-3 binds cell-associated galectin-9 which induces TIM-3 Tyr phosphorylation and proapoptotic signaling(10, 13). TIM-3 functions as a negative regulator of Th1 cell activity. Its blockade results in increased IFN-gamma production, Th1 cell proliferation, and cytotoxicity (5, 7, 12, 14). TIM-3 may play a role in regulatory T cell development, (7) inflammation, (15) and immune tolerance (5, 13, 14). Soluble mouse TIM-3 has been shown to inhibit anti-tumor effector T cell responses and to enhance autoimmune reactions (6, 7).
- Anderson, A.C. and D.E. Anderson (2006) Curr. Opin. Immunol. 18:665.
- Mariat, C. et al. (2005) Phil. Trans. R. Soc. B 360:1681.
- Meyers, J.H. et al. (2005) Trends Mol. Med. 11:362.
- Monney, L. et al. (2002) Nature 415:536.
- Sabatos, C.A. et al. (2003) Nat. Immunol. 4:1102.
- Geng, H. et al. (2006) J. Immunol. 176:1411.
- Sanchez-Fueyo, A. et al. (2003) Nat. Immunol. 4:1093.
- Khademi, M. et al. (2004) J. Immunol. 172:7169.
- Wiener, Z. et al. (2007) J. Invest. Dermatol. 127:906.
- van de Weyer, P.S. et al. (2006) Biochem. Biophys. Res. Commun. 351:571.
- Gielen, A.W. et al. (2005) J. Neuroimmunol. 164:93.
- Oikawa, T. et al. (2006) J. Immunol. 177:4281.
- Zhu, C. et al. (2005) Nat. Immunol. 6:1245.
- Koguchi, K. et al. (2006) J. Exp. Med. 203:1413.
- Frisancho-Kiss, S. et al. (2006) J. Immunol. 176:6411.
Product Datasheets
Citations for Mouse TIM-3 PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 7
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PD-1 combination therapy with IL-2 modifies CD8+ T cell exhaustion program
Authors: M Hashimoto, K Araki, MA Cardenas, P Li, RR Jadhav, HT Kissick, WH Hudson, DJ McGuire, RC Obeng, A Wieland, J Lee, DT McManus, JL Ross, SJ Im, J Lee, JX Lin, B Hu, EE West, CD Scharer, GJ Freeman, AH Sharpe, SS Ramalingam, A Pellerin, V Teichgräbe, WJ Greenleaf, C Klein, JJ Goronzy, P Umaña, WJ Leonard, KA Smith, R Ahmed
Nature, 2022-09-28;610(7930):173-181.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
DNA Methylation Patterns in CD 4 + T Cells of Na�ve and Influenza A Virus-Infected Mice Developmentally Exposed to an Aryl Hydrocarbon Receptor Ligand
Authors: CG Burke, JR Myers, CM Post, LA Boulé, BP Lawrence
Environmental health perspectives, 2021-01-15;129(1):17007.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Intracellular Galectin-3 Is Essential for OX40-Mediated Memory CD8+ T Cell Development
Authors: MF Amani, AS Rolig, WL Redmond
J. Immunol., 2020-08-26;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
BATF3-dependent dendritic cells drive both effector and regulatory T-cell responses in bacterially infected tissues
Authors: IC Arnold, X Zhang, M Artola-Bor, A Fallegger, P Sander, P Johansen, A Müller
PLoS Pathog., 2019-06-12;15(6):e1007866.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Enhanced Tim3 activity improves survival after influenza infection.
Authors: Cho J, Roche M, Sandall B, Brass A, Seed B, Xavier R, Medoff B
J Immunol, 2012-08-08;189(6):2879-89.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Influence of galectin-9/Tim-3 interaction on herpes simplex virus-1 latency.
Authors: Reddy PB, Sehrawat S, Suryawanshi A
J. Immunol., 2011-10-21;187(11):5745-55.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Role of Tim-3/galectin-9 inhibitory interaction in viral-induced immunopathology: shifting the balance toward regulators.
Authors: Sehrawat S, Suryawanshi A, Hirashima M, Rouse BT
J. Immunol., 2009-03-01;182(5):3191-201.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
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