Mouse SPARC Antibody Summary
Ala18-Ile302
Accession # P07214
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Mouse SPARC by Western Blot. Western blot shows lysates of C2C12 mouse myoblast cell line and mouse placenta tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse SPARC Monoclonal Antibody (Catalog # MAB942) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for SPARC at approximately 35-37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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SPARC/Osteonectin in Mouse Ovary. SPARC/Osteonectin was detected in perfusion fixed frozen sections of mouse ovary using Mouse SPARC/Osteonectin Monoclonal Antibody (Catalog # MAB942) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SPARC
SPARC, an acronym for “secreted protein, acidic and rich in cysteine”, is also known as osteonectin or BM-40 (1‑5). It is the founding member of a family of secreted matricellular proteins with similar domain structure. The 302 amino acid (aa), 43 kDa protein contains a 17 aa signal sequence, an N-terminal acidic region that binds calcium, a follistatin domain containing Kazal-like sequences, and a C-terminal extracellular calcium (EC) binding domain with two EF-hand motifs (1‑5). Crystal structure shows that residues implicated in cell binding, inhibition of cell spreading and disassembly of focal adhesions cluster on one face of SPARC, while a collagen binding epitope and an N-glycosylation site are opposite this face (6). SPARC is produced by fibroblasts, capillary endothelial cells, platelets and macrophages, especially in areas of tissue morphogenesis and remodeling (3, 7). SPARC shows context-specific effects, but generally inhibits adhesion, spreading and proliferation, and promotes collagen matrix formation (3‑5). For endothelial cells, SPARC disrupts focal adhesions and binds and sequesters PDGF and VEGF (3‑5). SPARC is abundantly expressed in bone, where it promotes osteoblast differentiation and inhibits adipogenesis (5, 8). SPARC is potentially cleaved by metalloproteinases, producing an angiogenic peptide that includes the copper-binding sequence KGHK (7). Paradoxically, SPARC is highly expressed in many tumor types, yet expression mainly decreases the likelihood of metastasis and confers sensitivity to chemotherapy and radiation (4, 9, 10). Stabilin-1, which is expressed on alternately activated macrophages, is the first SPARC receptor to be identified. It binds the SPARC EC domain and mediates endocytosis for degradation (11). Mature mouse SPARC shows 97%, 92%, 92%, 92% and 83% aa identity with rat, human, dog, cow and chick SPARC, respectively.
- Lankat-Buttgereit, B. et al. (1988) FEBS Lett. 236:352.
- McVey, J.H. et al. (1988) J. Biol. Chem. 263:11111.
- Sage, H. et al. (1989) J. Cell Biol. 109:341.
- Framson, P.E. and E.H. Sage (2004) J. Cell. Biochem. 92:679.
- Alford, A.I. and K.D. Hankenson (2006) Bone 38:749.
- Hohenester, E. et al. (1997) EMBO J. 16:3778.
- Sage, E.H. et al. (2003) J. Biol. Chem. 278:37849.
- Delany, A.M. et al. (2003) Endocrinology 144:2588.
- Koblinski, J.E. et al. (2005) Cancer Res. 65:7370.
- Tai, I.T. et al. (2005) J. Clin. Invest. 115:1492.
- Kzhyshkowska, J. et al. (2006) J. Immunol. 176:5825.
Product Datasheets
Citations for Mouse SPARC Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 9
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Secreted protein acidic and rich in cysteine (SPARC) knockout mice have greater outflow facility
Authors: L Yu, Y Zheng, BJ Liu, MH Kang, JC Millar, DJ Rhee
PLoS ONE, 2020-11-04;15(11):e0241294.
Species: Mouse, Transgenic Mouse
Sample Types: Whole Tissue
Applications: IHC -
Implication of SPARC in the modulation of the extracellular matrix and mitochondrial function in muscle cells
Authors: A Melouane, A Carbonell, M Yoshioka, J Puymirat, J St-Amand
PLoS ONE, 2018-02-08;13(2):e0192714.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization -
Blockade of Astrocytic Calcineurin/NFAT Signaling Helps to Normalize Hippocampal Synaptic Function and Plasticity in a Rat Model of Traumatic Brain Injury
Authors: Jennifer L. Furman, Pradoldej Sompol, Susan D. Kraner, Melanie M. Pleiss, Esther J. Putman, Jacob Dunkerson et al.
The Journal of Neuroscience
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SPARC Expression Did Not Predict Efficacy of nab-Paclitaxel plus Gemcitabine or Gemcitabine Alone for Metastatic Pancreatic Cancer in an Exploratory Analysis of the Phase III MPACT Trial.
Authors: Hidalgo M, Plaza C, Musteanu M, Illei P, Brachmann C, Heise C, Pierce D, Lopez-Casas P, Menendez C, Tabernero J, Romano A, Wei X, Lopez-Rios F, Von Hoff D
Clin Cancer Res, 2015-07-13;21(21):4811-8.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Clever-1/stabilin-1 controls cancer growth and metastasis.
Authors: Karikoski M, Marttila-Ichihara F, Elima K, Rantakari P, Hollmen M, Kelkka T, Gerke H, Huovinen V, Irjala H, Holmdahl R, Salmi M, Jalkanen S
Clin Cancer Res, 2014-10-15;20(24):6452-64.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Stromal disrupting effects of nab-paclitaxel in pancreatic cancer
Authors: R Alvarez, M Musteanu, E Garcia-Garcia, P P Lopez-Casas, D Megias, C Guerra et al.
British Journal of Cancer
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SPARC mediates early extracellular matrix remodeling following myocardial infarction.
Authors: McCurdy S, Dai Q, Zhang J, Zamilpa R, Ramirez T, Dayah T, Nguyen N, Jin Y, Bradshaw A, Lindsey M
Am J Physiol Heart Circ Physiol, 2011-05-20;301(2):H497-505.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Secreted protein acidic and rich in cysteine deficiency ameliorates renal inflammation and fibrosis in angiotensin hypertension.
Authors: Socha MJ, Manhiani M, Said N, Imig JD, Motamed K
Am. J. Pathol., 2007-08-23;171(4):1104-12.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Absence of SPARC in lens epithelial cells results in altered adhesion and extracellular matrix production in vitro.
Authors: Weaver MS, Sage EH, Yan Q
J. Cell. Biochem., 2006-02-01;97(2):423-32.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC
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