Mouse Serpin E1/PAI-1 Biotinylated Antibody

Catalog #: BAF3828 Datasheet

Catalog #	Availability	Size / Price	Qty
BAF3828

Detection of Mouse Serpin E1/PAI‑1 by Western Blot.

1 Image

All Serpin E1/PAI-1 Antibodies

Product Details

FAQs

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Serpin E1/PAI-1 Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Serpin E1/PAI‑1 in Western blots. In Western blots, approximately 20% cross-reactivity with recombinant human (rh) Serpin E1 is observed, and less than 2% cross-reactivity with recombinant mouse (rm) Serpin E2 and rmSerpin I1 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Serpin E1/PAI‑1
Leu24-Ser402
Accession # AAH54091

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Mouse Serpin E1/PAI‑1 by Western Blot. Western blot shows lysates of mouse placenta tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse Serpin E1/PAI-1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3828) followed by Streptavidin-HRP (Catalog # DY998) in 3% BSA. A specific band was detected for Serpin E1/PAI-1 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions.

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin E1/PAI-1

Serpin E1, also known as Plasminogen Activator Inhibitor-1 (PAI-1), is the principal inhibitor of urokinase-type and tissue-type plasminogen activators. It plays a major role in many processes such as angiogenesis, tumor invasion, and obesity. The amino acid sequence of mouse PAI-1 is 89%, 79% and 76% identical to that of rat, human/chimpanzee and dog.

Long Name

Plasminogen Activator Inhibitor

Entrez Gene IDs

5054 (Human); 18787 (Mouse)

Alternate Names

Endothelial plasminogen activator inhibitor; Nexin; PAI1; PAI-1; PAI1PAI-1; PAISerpin E1; PLANH1; PLANH1plasminogen activator inhibitor 1; serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogenactivator inhibitor type 1), member 1; Serpin E1; serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitortype 1), member 1

Product Datasheets

Product Datasheet

Related Research Areas

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Mouse Serpin E1/PAI-1 Biotinylated Antibody

There are currently no reviews for this product. Be the first to review Mouse Serpin E1/PAI-1 Biotinylated Antibody and earn rewards!

Have you used Mouse Serpin E1/PAI-1 Biotinylated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review