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Mouse Serpin E1/PAI-1 Antibody

Catalog #: AF3828
9 Citations Datasheet
Catalog # Availability Size / Price Qty
AF3828
AF3828-SP
Detection of Mouse Serpin E1/PAI‑1 by Western Blot.
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Product Details
Citations (9)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Serpin E1/PAI-1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Serpin E1 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human Serpin E1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Serpin E1/PAI-1
Leu24-Ser402
Accession # AAH54091
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.25 µg/mL
See below
Simple Western
12.5 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Mouse Serpin E1/PAI‑1, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse Serpin E1/PAI-1 antibody by Western Blot. View Larger

Detection of Mouse Serpin E1/PAI‑1 by Western Blot. Western blot shows lysates of mouse placenta tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse Serpin E1/PAI-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3828) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Serpin E1/PAI-1 at approximately 45-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Mouse Serpin E1/PAI-1 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Mouse Serpin E1/PAI‑1 by Simple WesternTM. Simple Western lane view shows lysates of mouse placenta tissue, loaded at 0.2 mg/mL. A specific band was detected for Serpin E1/PAI-1 at approximately 56 kDa (as indicated) using 12.5 µg/mL of Goat Anti-Mouse Serpin E1/PAI-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3828) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin E1/PAI-1

Serpin E1, also known as Plasminogen Activator Inhibitor-1 (PAI-1), is the principal inhibitor of urokinase-type and tissue-type plasminogen activators. It plays a major role in many processes such as angiogenesis, tumor invasion, and obesity. The amino acid sequence of mouse PAI-1 is 89%, 79% and 76% identical to that of rat, human/chimpanzee and dog.

Long Name
Plasminogen Activator Inhibitor
Entrez Gene IDs
5054 (Human); 18787 (Mouse)
Alternate Names
Endothelial plasminogen activator inhibitor; Nexin; PAI1; PAI-1; PAI1PAI-1; PAISerpin E1; PLANH1; PLANH1plasminogen activator inhibitor 1; serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogenactivator inhibitor type 1), member 1; Serpin E1; serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitortype 1), member 1

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Citations for Mouse Serpin E1/PAI-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. (Pro)renin Receptor Is an Amplifier of Wnt/ beta -Catenin Signaling in Kidney Injury and Fibrosis
    Authors: Zhen Li, Lili Zhou, Yongping Wang, Jinhua Miao, Xue Hong, Fan Fan Hou et al.
    Journal of the American Society of Nephrology
  2. beta -Arrestin Deficiency Protects Against Pulmonary Fibrosis in Mice and Prevents Fibroblast Invasion of Extracellular Matrix
    Authors: Alysia Kern Lovgren, Jeffrey J. Kovacs, Ting Xie, Erin N. Potts, Yuejuan Li, W. Michael Foster et al.
    Science Translational Medicine
  3. Reduced secretion of neuronal growth regulator 1 contributes to impaired adipose-neuronal crosstalk in obesity
    Authors: E Duregotti, CM Reumiller, U Mayr, M Hasman, LE Schmidt, SA Burnap, K Theofilato, J Barallobre, A Beran, M Grandoch, A Viviano, M Jahangiri, M Mayr
    Nature Communications, 2022-11-25;13(1):7269.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  4. Acquired Deficiency of Peroxisomal Dicarboxylic Acid Catabolism is a Metabolic Vulnerability in Hepatoblastoma
    Authors: H Wang, J Lu, X Chen, M Schwalbe, JE Gorka, JA Mandel, J Wang, ES Goetzman, S Ranganatha, SF Dobrowolsk, EV Prochownik
    The Journal of Biological Chemistry, 2021-01-13;0(0):100283.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  5. Klotho retards renal fibrosis through targeting mitochondrial dysfunction and cellular senescence in renal tubular cells
    Authors: J Miao, J Huang, C Luo, H Ye, X Ling, Q Wu, W Shen, L Zhou
    Physiological Reports, 2021-01-01;9(2):e14696.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  6. C-X-C motif chemokine receptor 4 aggravates renal fibrosis through activating JAK/STAT/GSK3&beta/&beta-catenin pathway
    Authors: Y Liu, Q Feng, J Miao, Q Wu, S Zhou, W Shen, Y Feng, FF Hou, Y Liu, L Zhou
    J. Cell. Mol. Med., 2020-03-02;0(0):.
    Species: Human
    Sample Types: Cell Culture Lysates
    Applications: Western Blot
  7. Wnt/?-catenin/RAS signaling mediates age-related renal fibrosis and is associated with mitochondrial dysfunction
    Authors: J Miao, J Liu, J Niu, Y Zhang, W Shen, C Luo, Y Liu, C Li, H Li, P Yang, Y Liu, FF Hou, L Zhou
    Aging Cell, 2019-07-18;0(0):e13004.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  8. Thymosin beta4 and its degradation product, Ac-SDKP, are novel reparative factors in renal fibrosis.
    Authors: Zuo Y, Chun B, Potthoff S, Kazi N, Brolin T, Orhan D, Yang H, Ma L, Kon V, Myohanen T, Rhaleb N, Carretero O, Fogo A
    Kidney Int, 2013-06-05;84(6):1166-75.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Exogenous Wnt1 Prevents Acute Kidney Injury and Its Subsequent Progression to Chronic Kidney Disease
    Authors: Xue Hong, Yanni Zhou, Dedong Wang, Fuping Lyu, Tianjun Guan, Youhua Liu et al.
    Frontiers in Physiology

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