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Mouse S1P1/EDG-1 APC-conjugated Antibody

Catalog #: FAB7089A
7 Citations 2 Reviews Datasheet
Catalog # Availability Size / Price Qty
FAB7089A-100UG
Detection of S1P1/EDG‑1 in HEK293 Human Cell Line Transfected with Mouse S1P1/EDG-1 and eGFP by Flow Cytometry.
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Product Details
Citations (7)
FAQs
Supplemental Products
Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse S1P1/EDG-1 APC-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse S1P1/EDG‑1 peptide in direct ELISAs.
Source
Monoclonal Rat IgG2A Clone # 713412
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse S1P1/EDG‑1 synthetic peptide (T4-H28)
Accession # O08530
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of S1P<sub>1</sub>/EDG-1 antibody in HEK293 Human Cell Line Transfected with Mouse S1P<sub>1</sub>/EDG-1 and eGFP antibody by Flow Cytometry. View Larger

Detection of S1P1/EDG‑1 in HEK293 Human Cell Line Transfected with Mouse S1P1/EDG-1 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with mouse S1P1/EDG-1 and eGFP was stained with either (A) Rat Anti-Mouse S1P1/EDG-1 APC-conjugated Monoclonal Antibody (Catalog # FAB7089A) or (B) Rat IgG2AAllophycocyanin Isotype Control (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of S1P<SUB>1</SUB>/EDG-1 antibody in Mouse Thymocytes antibody by Flow Cytometry. View Larger

Detection of S1P1/EDG‑1 in Mouse Thymocytes by Flow Cytometry. Mouse thymocytes were stained with Rat Anti-Mouse CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB554P) and either (A) Rat Anti-Mouse S1P1/EDG-1 APC-conjugated Monoclonal Antibody (Catalog # FAB7089A) or (B) Rat IgG2AAllophycocyanin Isotype Control (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: S1P1/EDG-1

S1P1 (sphingosine 1-phosphate receptor-1), also known as EDG-1 (endothelial differentiation, G-protein coupled receptor-1) or S1PR1 (sphingosine-1-phosphate receptor 1) is a widely expressed, 37-40 kDa, G protein coupled receptor within the S1P subfamily of the EDG family. S1P1 is one of five receptors for the bioactive lipid S1P and mediates most of S1P effects on angiogenesis, vascular maturation, and cell migration, especially T cell egress from lymphoid organs. Human and mouse S1P1 share 84% amino acid identity within the N-terminal extracellular portion used as an immunogen.

Long Name
Endothelial Differentiation Gene 1
Entrez Gene IDs
1901 (Human); 13609 (Mouse)
Alternate Names
CD363 antigen; CD363; CHEDG1; D1S3362; EDG1; EDG-1; EDG1edg-1; Endothelial differentiation G-protein coupled receptor 1; endothelial differentiation, sphingolipid G-protein-coupled receptor, 1; FLJ58121; S1P receptor 1; S1P receptor Edg-1; S1P1; S1P1ECGF1; S1PR1; sphingosine 1-phosphate receptor 1; sphingosine 1-phosphate receptor EDG1; Sphingosine 1-phosphate receptor Edg-1; sphingosine-1-phosphate receptor 1

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Citations for Mouse S1P1/EDG-1 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Targeting the MR1-MAIT cell axis improves vaccine efficacy and affords protection against viral pathogens
    Authors: Rashu, R;Ninkov, M;Wardell, CM;Benoit, JM;Wang, NI;Meilleur, CE;D'Agostino, MR;Zhang, A;Feng, E;Saeedian, N;Bell, GI;Vahedi, F;Hess, DA;Barr, SD;Troyer, RM;Kang, CY;Ashkar, AA;Miller, MS;Haeryfar, SMM;
    PLoS pathogens
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. S1PR1 attenuates pulmonary fibrosis by inhibiting EndMT and improving endothelial barrier function
    Authors: Xiong, W;Chen, S;Xiang, H;Zhao, S;Xiao, J;Li, J;Liu, Y;Shu, Z;Ouyang, J;Zhang, J;Liu, H;Wang, X;Zou, H;Chen, Y;Chen, A;Lu, H;
    Pulmonary pharmacology & therapeutics
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Sequestration of T cells in bone marrow in the setting of glioblastoma and other intracranial tumors
    Authors: P Chongsathi, C Jackson, S Koyama, F Loebel, X Cui, SH Farber, K Woroniecka, AA Elsamadicy, CA Dechant, HR Kemeny, L Sanchez-Pe, TA Cheema, NC Souders, JE Herndon, JV Coumans, JI Everitt, BV Nahed, JH Sampson, MD Gunn, RL Martuza, G Dranoff, WT Curry, PE Fecci
    Nat. Med., 2018-08-13;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Impaired lymphocyte trafficking in mice deficient in the kinase activity of PKN1
    Authors: R Mashud, A Nomachi, A Hayakawa, K Kubouchi, S Danno, T Hirata, K Matsuo, T Nakayama, R Satoh, R Sugiura, M Abe, K Sakimura, S Wakana, H Ohsaki, S Kamoshida, H Mukai
    Sci Rep, 2017-08-09;7(1):7663.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. IL4RA on lymphatic endothelial cells promotes T cell egress during sclerodermatous graft versus host disease
    JCI Insight, 2016-08-04;1(12):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Targeting of Ly9 (CD229) Disrupts Marginal Zone and B1 B Cell Homeostasis and Antibody Responses.
    Authors: Cuenca M, Romero X, Sintes J, Terhorst C, Engel P
    J Immunol, 2015-12-14;196(2):726-37.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Intravenous gammaglobulin inhibits encephalitogenic potential of pathogenic T cells and interferes with their trafficking to the central nervous system, implicating sphingosine-1 phosphate receptor 1-mammalian target of rapamycin axis.
    Authors: Othy S, Hegde P, Topcu S, Sharma M, Maddur M, Lacroix-Desmazes S, Bayry J, Kaveri S
    J Immunol, 2013-03-22;190(9):4535-41.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

  1. Can staining with Mouse S1P1/EDG-1 conjugated Antibody, catalog # FAB7089*,  be carried out at 4°C? 

    • There is evidence that G protein family proteins require staining at room temperature (RT) or even 37 °C, so this is what we would recommend.  Staining for 45 minutes is optimal with this antibody. 

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Reviews for Mouse S1P1/EDG-1 APC-conjugated Antibody

Average Rating: 4 (Based on 2 Reviews)

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Mouse S1P1/EDG-1 APC-conjugated Antibody
By Anonymous on 10/25/2018
Application: Flow Sample Tested: Spleen tissue Species: Mouse
Mouse S1P1/EDG-1 APC-conjugated Antibody
By Anonymous on 08/24/2017
Application: Flow Sample Tested: Adult splenocytes,Adult thymocytes,bone marrow Species: Mouse