Mouse/Rat TLR7 Alexa Fluor® 647-conjugated Antibody

Catalog #: FAB7156R Datasheet

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FAB7156R-100UG

All TLR7 Antibodies

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Mouse/Rat TLR7 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity

Mouse, Rat

Specificity

Detects mouse TLR7 in direct ELISAs and mouse and rat TLR7 in Western blots. In direct ELISAs, no cross-reactivity with recombinant human TLR7 or recombinant mouse TLR8 is observed.

Source

Monoclonal Rat IgG2a Clone # 726606

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant mouse TLR7
Asn275-Phe444
Accession # P58681

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: TLR7

TLR7 (Toll-like receptor 7) is an approximately 130 kDa type I transmembrane glycoprotein and member of the Toll-like receptor family. Mouse TLR7 cDNA encodes 1050 amino acids (aa) including a 26 aa signal sequence and a 811 aa extracellular domain (ECD) with 28 leucine-rich repeats (LRR). Within the region used as an immunogen (LRR 10-16), mouse TLR7 shares 73% and 88% aa identity with human and rat TLR7, respectively. TLR7 mRNA is detected in brain, placenta, spleen, stomach, small intestine, lung, and in plasmacytoid dendritic cells, B cells and eosinophils. TLR7 participates in the innate immune response to microbial agents by recognizing viral and nonviral single stranded RNA.

Long Name

Toll-like Receptor 7

Entrez Gene IDs

51284 (Human); 170743 (Mouse); 317468 (Rat)

Alternate Names

TLR7; toll-like receptor 7