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Mouse/Rat MMP-2 Antibody

Catalog #: AF1488
36 Citations Datasheet
Catalog # Availability Size / Price Qty
AF1488
AF1488-SP
MMP‑2 in Mouse Thymus.
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Product Details
Citations (36)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse/Rat MMP-2 Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects mouse and rat MMP-2 in direct ELISAs and Western blots. In direct ELISAs, less than 40% cross-reactivity with recombinant human MMP-2 is observed and less than 1% cross-reactivity with recombinant mouse (rm) MMP-3 and rmMMP-9 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse MMP-2
Ile34-Cys662
Accession # P33434
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse/Rat MMP‑2 (Catalog # 924-MP)
Immunohistochemistry
5-15 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Mouse/Rat MMP‑2 (Catalog # 924‑MP), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry MMP-2 antibody in Mouse Thymus by Immunohistochemistry (IHC-Fr). View Larger

MMP‑2 in Mouse Thymus. MMP-2 was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse/ Rat MMP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1488) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Immunohistochemistry-Paraffin Detection of Mouse MMP-2 by Immunohistochemistry-Paraffin View Larger

Detection of Mouse MMP-2 by Immunohistochemistry-Paraffin MMP2, MMP9, and RANKL expression in AAAs.A. Immunohistochemical staining for indicated proteins of serial sections of aortas one week after CaCl2 treatment. Elastic van Gieson staining is also shown. SM alpha -actin and F4/80 were stained to locate SMCs and macrophages, respectively. Shown are representative images of 4 or more samples in each group. Scale bars, 50 µm. B. Relative positive staining area of MMP2, MMP9, and RANKL in sections from control diet and EPA diet groups. n = 4–5. *P<0.05. Image collected and cropped by CiteAb from the following open publication (https://dx.plos.org/10.1371/journal.pone.0096286), licensed under a CC-BY license. Not internally tested by R&D Systems.

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMP-2

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-2 (gelatinase A), a type IV collagenase, can degrade a broad range of substrates including type IV, V, VII and X collagens as well as elastin and fibronectin. It is believed to act synergistically with interstitial collagenase (MMP-1) in the degradation of fibrillar collagens as it degrades their denatured gelatin forms. MMP-2 has been shown to be associated with many connective tissue cells as well as neutrophils, macrophages and monocytes. Structurally, MMP-2 may be divided into several distinct domains: a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a fibronectin-like domain thought to play a role in substrate targeting; and a carboxyl terminal (hemopexin-like) domain containing 2 N-linked glycosylation sites. The amino acid sequences of the proenzymes are identical between mouse and rat.  

Long Name
Matrix Metalloproteinase 2
Entrez Gene IDs
4313 (Human); 17390 (Mouse)
Alternate Names
72 kDa gelatinase; CLG4; CLG4A72 kDa type IV collagenase; collagenase type IV-A; EC 3.4.24; EC 3.4.24.24; Gelatinase A; matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase); matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IVcollagenase); Matrix metalloproteinase-2; matrix metalloproteinase-II; MMP2; MMP-2; MMP-II; MONA; neutrophil gelatinase; TBE-1matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase)

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Citations for Mouse/Rat MMP-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

36 Citations: Showing 1 - 10
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  1. Gelatinases Cleave Dentin Sialoprotein Intracellularly
    Authors: X Gou, Y Xue, H Zheng, G Yang, S Chen, Z Chen, G Yuan
    Front Physiol, 2020-06-25;11(0):686.
  2. Kunming mouse strain is less susceptible to elastase induced abdominal aortic aneurysms
    Authors: Liu H, Tian K, Xia C et al.
    Animal Model Exp Med
  3. C-reactive protein deficiency ameliorates experimental abdominal aortic aneurysms
    Authors: Fu Y, Liu H, Li K et al.
    Front Immunol
  4. Genetic deficiency of protein inhibitor of activated STAT3 suppresses experimental abdominal aortic aneurysms
    Authors: Weilai Fu, Haole Liu, Panpan Wei, Congcong Xia, Qingqing Yu, Kangli Tian et al.
    Frontiers in Cardiovascular Medicine
  5. Inflammatory Role of Milk Fat Globule–Epidermal Growth Factor VIII in Age‐Associated Arterial Remodeling
    Authors: Leng Ni, Lijuan Liu, Wanqu Zhu, Richard Telljohann, Jing Zhang, Robert E. Monticone et al.
    Journal of the American Heart Association
  6. Temporal and Quantitative Analysis of Aortic Immunopathologies in Elastase-Induced Mouse Abdominal Aortic Aneurysms
    Authors: K Tian, C Xia, H Liu, B Xu, P Wei, W Fu, M Lu, Y Li, Y Li, D Cheng, E Liu, S Zhao
    Journal of Immunology Research, 2021-11-16;2021(0):6297332.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Nicotine Exacerbates TAAD Formation Induced by Smooth Muscle-Specific Deletion of the TGF-beta Receptor 2
    Authors: C Chun, X Qi, F Wang, KB Madrid, LA Saldarriag, MR Fisch, ML Brantly, GR Upchurch, Z Jiang
    Journal of Immunology Research, 2021-10-01;2021(0):6880036.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. Tendon and motor phenotypes in the Crtap-/- mouse model of recessive osteogenesis imperfecta
    Authors: Matthew William Grol, Nele A Haelterman, Joohyun Lim, Elda M Munivez, Marilyn Archer, David M Hudson et al.
    eLife
  9. miR-146b Functions as an Oncogene in Oral Squamous Cell Carcinoma by Targeting HBP1
    Authors: Kui Li, Zheng Zhou, Ju Li, Rui Xiang
    Technol Cancer Res Treat
  10. Deficiency of peroxiredoxin 2 exacerbates angiotensin II-induced abdominal aortic aneurysm
    Authors: SJ Jeong, MJ Cho, NY Ko, S Kim, IH Jung, JK Min, SH Lee, JG Park, GT Oh
    Exp. Mol. Med., 2020-09-14;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. Matrix metalloproteinases activation in Toxocara canis induced pulmonary pathogenesis
    Authors: CY Lu, SC Lai, HH Lee, HT Chien, KP Lan, KM Chen
    J Microbiol Immunol Infect, 2020-08-11;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  12. ATRQ β-001 Vaccine Prevents Experimental Abdominal Aortic Aneurysms
    Authors: H Zhang, M Liao, M Cao, Z Qiu, X Yan, Y Zhou, H Wu, Y Wang, J Zheng, J Ding, M Wang, Y Liao, X Chen
    J Am Heart Assoc, 2019-09-12;8(18):e012341.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  13. Tea Polysaccharide Prevents Colitis-Associated Carcinogenesis in Mice by Inhibiting the Proliferation and Invasion of Tumor Cells
    Authors: LQ Liu, HS Li, SP Nie, MY Shen, JL Hu, MY Xie
    Int J Mol Sci, 2018-02-08;19(2):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  14. Chemopreventive Potential of 2,3,5,4'-Tetrahydroxystilbene-2-O-?-D-glucoside on the Formation of Aberrant Crypt Foci in Azoxymethane-Induced Colorectal Cancer in Rats
    Authors: CL Lin, JH Jeng, CC Wu, SL Hsieh, GC Huang, W Leung, CT Lee, CY Chen, CH Lee
    Biomed Res Int, 2017-11-07;2017(0):3634915.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  15. Thrombospondin-2 promotes prostate cancer bone metastasis by the up-regulation of matrix metalloproteinase-2 through down-regulating miR-376c expression
    Authors: PC Chen, CH Tang, LW Lin, CH Tsai, CY Chu, TH Lin, YL Huang
    J Hematol Oncol, 2017-01-25;10(1):33.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  16. Smooth muscle cell-specific Tgfbr1 deficiency attenuates neointimal hyperplasia but promotes an undesired vascular phenotype for injured arteries
    Physiol Rep, 2016-12-01;4(23):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  17. sFRP2 activates Wnt/ beta -catenin signaling in cardiac fibroblasts: differential roles in cell growth, energy metabolism, and extracellular matrix remodeling
    Authors: Huey Lin, Mia Angeli, Kwang Jin Chung, Chukwuemeka Ejimadu, Angelica Rivera Rosa, Techung Lee
    American Journal of Physiology-Cell Physiology
  18. Molecular hydrogen in drinking water protects against neurodegenerative changes induced by traumatic brain injury.
    Authors: Dohi, Kenji, Kraemer, Brian C, Erickson, Michelle, McMillan, Pamela J, Kovac, Andrej, Flachbartova, Zuzana, Hansen, Kim M, Shah, Gul N, Sheibani, Nader, Salameh, Therese, Banks, William
    PLoS ONE, 2014-09-24;9(9):e108034.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  19. The omega-3 polyunsaturated fatty acid, eicosapentaenoic acid, attenuates abdominal aortic aneurysm development via suppression of tissue remodeling.
    Authors: Wang J, Eguchi K, Matsumoto S, Fujiu K, Komuro I, Nagai R, Manabe I
    PLoS ONE, 2014-05-05;9(5):e96286.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  20. Deletion of the EphA2 receptor exacerbates myocardial injury and the progression of ischemic cardiomyopathy.
    Authors: O'Neal W, Griffin W, Kent S, Faiz F, Hodges J, Vuncannon J, Virag J
    Front Physiol, 2014-04-24;5(0):132.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  21. KLF4 regulates abdominal aortic aneurysm morphology and deletion attenuates aneurysm formation.
    Authors: Salmon M, Johnston W, Woo A, Pope N, Su G, Upchurch G, Owens G, Ailawadi G
    Circulation, 2013-09-10;128(11):S163-74.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  22. G protein-coupled receptor kinase 6 deficiency promotes angiogenesis, tumor progression, and metastasis.
    Authors: Raghuwanshi S, Smith N, Rivers E, Thomas A, Sutton N, Hu Y, Mukhopadhyay S, Chen X, Leung T, Richardson R
    J Immunol, 2013-04-15;190(10):5329-36.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development (Capture)
  23. Site-Specific Expression of Gelatinolytic Activity during Morphogenesis of the Secondary Palate in the Mouse Embryo.
    Authors: Gkantidis, Nikolaos, Blumer, Susan, Katsaros, Christos, Graf, Daniel, Chiquet, Matthias
    PLoS ONE, 2012-10-16;7(10):e47762.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  24. Vitamin D receptor agonists increase klotho and osteopontin while decreasing aortic calcification in mice with chronic kidney disease fed a high phosphate diet.
    Authors: Lau, Wei Ling, Leaf, Elizabet, Hu, Ming Cha, Takeno, Marc M, Kuro-o, Makoto, Moe, Orson W, Giachelli, Cecilia
    Kidney Int, 2012-08-29;82(12):1261-70.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  25. Activated protein C inhibits pancreatic islet inflammation, stimulates T regulatory cells, and prevents diabetes in non-obese diabetic (NOD) mice.
    Authors: Xue M, Dervish S, Harrison LC, Fulcher G, Jackson CJ
    J. Biol. Chem., 2012-03-23;287(20):16356-64.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  26. Tissue inhibitor of matrix metalloproteinase 1 (TIMP1) controls adipogenesis in obesity in mice and in humans.
    Authors: Meissburger B, Stachorski L, Roder E, Rudofsky G, Wolfrum C
    Diabetologia, 2011-03-25;54(6):1468-79.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  27. Elastin degradation and vascular smooth muscle cell phenotype change precede cell loss and arterial medial calcification in a uremic mouse model of chronic kidney disease.
    Authors: Pai A, Leaf EM, El-Abbadi M, Giachelli CM
    Am. J. Pathol., 2011-02-01;178(2):764-73.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  28. High dietary intake of retinol leads to bone marrow hypoxia and diaphyseal endosteal mineralization in rats.
    Authors: Lind T, Lind PM, Jacobson A, Hu L, Sundqvist A, Risteli J, Yebra-Rodriguez A, Rodriguez-Navarro A, Andersson G, Melhus H
    Bone, 2010-10-28;48(3):496-506.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  29. Arhgef1 regulates alpha5beta1 integrin-mediated matrix metalloproteinase expression and is required for homeostatic lung immunity
    Authors: John M. Hartney, Jeanette Brown, Hong Wei Chu, Ling Yi Chang, Roberta Pelanda, Raul M. Torres
    The American Journal of Pathology
  30. Interleukin-1 receptor type I signaling critically regulates infarct healing and cardiac remodeling.
    Authors: Bujak M, Dobaczewski M, Chatila K, Mendoza LH, Li N, Reddy A, Frangogiannis NG
    Am. J. Pathol., 2008-06-05;173(1):57-67.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  31. Genetic deficiency of heme oxygenase-1 impairs functionality and form of an arteriovenous fistula in the mouse.
    Authors: Juncos JP, Tracz MJ, Croatt AJ, Grande JP, Ackerman AW, Katusic ZS, Nath KA
    Kidney Int., 2008-03-26;74(1):47-51.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  32. A GMCSF and IL-15 fusokine leads to paradoxical immunosuppression in vivo via asymmetrical JAK/STAT signaling through the IL-15 receptor complex.
    Authors: Rafei M, Wu JH, Annabi B, Lejeune L, Francois M, Galipeau J
    Blood, 2006-11-02;109(5):2234-42.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  33. Characterization of lymphangiogenesis in a model of adult skin regeneration.
    Authors: Rutkowski JM, Boardman KC, Swartz MA
    Am. J. Physiol. Heart Circ. Physiol., 2006-04-28;291(3):H1402-10.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  34. Expression of matrix metalloproteinases subsequent to urogenital Chlamydia muridarum infection of mice.
    Authors: Ramsey KH, Sigar IM, Schripsema JH, Shaba N, Cohoon KP
    Infect. Immun., 2005-10-01;73(10):6962-73.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  35. Modulation of proteoglycan receptor PTP? enhances MMP-2 activity to promote recovery from multiple sclerosis
    Authors: F Luo, AP Tran, L Xin, C Sanapala, BT Lang, J Silver, Y Yang
    Nat Commun, 2018-10-08;9(1):4126.
  36. Liquiritigenin Protects Rats from Carbon Tetrachloride Induced Hepatic Injury through PGC-1a Pathway.
    Authors: Zhang Yiping, He Yuanqiao, Yu Hongbo et al.
    Evidence-Based Complementary and Alternative Medicine : ECam

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