Mouse/Rat CREB Antibody

Catalog #: MAB5435 1 Review Datasheet

Catalog #	Availability	Size / Price	Qty
MAB5435
MAB5435-SP

Detection of Mouse/Rat CREB by Western Blot.

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Mouse/Rat CREB Antibody Summary

Species Reactivity

Mouse, Rat

Specificity

The antibody detects endogenous mouse and rat CREB on Western blots.

Source

Monoclonal Rat IgG_2A Clone # 485422

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse CREB
Met1-Lys69
Accession # NP_001032815

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse/Rat CREB by Western Blot. Western blot shows lysates of A20 mouse B cell lymphoma cell line and M1 mouse myeloid leukemia cell line. PVDF membrane was probed with 1 µg/mL of Mouse/Rat CREB Monoclonal Antibody (Catalog # MAB5435) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CREB at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CREB

The cAMP response element binding protein (CREB), also known as CREB1, belongs to the bZIP family of transcription factors. Each bZIP family member contains a basic domain that mediates DNA binding and a leucine zipper domain that facilitates dimerization. Within the promoter of target genes, CREB dimers bind cAMP response elements, defined by the palindromic consensus sequence TGACGTCA. CREB becomes phosphorylated at specific residues in response to DNA damage and circadian cycles.

Long Name

cAMP Response Element-binding Protein

Entrez Gene IDs

1385 (Human); 12912 (Mouse)

Alternate Names

active transcription factor CREB; cAMP responsive element binding protein 1; cAMP-response element-binding protein-1; cAMP-responsive element-binding protein 1; CREB; CREB1; CREB-1; cyclic AMP-responsive element-binding protein 1; MGC9284; transactivator protein