Mouse/Rat CD31/PECAM-1 APC-conjugated Antibody Summary
Glu18-Lys590
Accession # Q08481
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of CD31/PECAM‑1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Goat Anti-Mouse/Rat CD31/PECAM-1 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB3628A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Detection of CD31/PECAM‑1 in Rat Splenocytes by Flow Cytometry. Rat splenocytes were stained with Goat Anti-Mouse/Rat CD31/PECAM-1 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB3628A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Detection of Mouse CD31/PECAM-1 by Flow Cytometry CD31hiEmcnhi vessel and bone formation decreased during ageing but increased in bone regeneration. A, Representative images of CD31 (green), EMCN (red) and Osterix (white) immunostaining. Scale bar, 100 μm. G, growth plate. B, Quantification of CD31 and EMCN positive vessel volume in distal femora. C, Quantitative analysis of Osterix‐positive (OSX+) osteoprogenitors in distal femora. D and E, FACS analysis dot plot (D) and quantification (E) of CD31hiEMCNhi ECs. F, Representative images of CD31 (green) and EMCN (red) immunostaining in bone regeneration area after femoral trabecular bone ablation. Nuclei, DAPI (blue). Scale bar, 100 μm. G and H, Representative μCT images (G) and quantitative μCT analysis (H) of bone regeneration after femoral trabecular bone ablation. Selected areas for the measurements of bone volume (BV)/tissue volume (TV) were indicated with a yellow square. Data are shown as mean ± SD, (n = 6 in B, C, E and H). *P < .05; **P < .01 by one‐way ANOVA Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32080957), licensed under a CC-BY license. Not internally tested by R&D Systems.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD31/PECAM-1
PECAM-1 (Platelet-Endothelial Cell Adhesion Molecule-1), also known as CD31, is a 130 kDa type I transmembrane glycoprotein adhesion molecule in the immunoglobulin superfamily (1, 2). Expression is restricted to cells involved in circulation, especially endothelial cells, platelets, monocytes, neutrophils and lymphocyte subsets. PECAM-1 is concentrated at cell-cell junctions and is required for Transendothelial Migration (TEM) (1-3). The Extracellular Domain (ECD) of PECAM-1 has ten potential N-linked glycosylation sites and six C2-type Ig-like domains, the first of which is critical for adhesion and extravasation (3, 4). The cytoplasmic domain contains Immunoregulatory Tyrosine-based Inhibitory and Switch Motifs (ITIM, ITSM) that mediate both inhibition and activation via phosphotyrosine-mediated engagement of SH2-containing signaling molecules (1, 5). Metalloproteinase-mediated ectodomain shedding occurs during apoptosis (6) but increased serum PECAM-1 ectodomain in HIV and active multiple sclerosis occurs independent of apoptosis (7, 8). In humans, expression of six isoforms with exon deletions in the cytoplasmic domain is tissue- and stage-specific, but full-length PECAM-1 is predominant. A form lacking the ITSM predominates in mouse (9). Mouse PECAM-1 ECD shows 77%, 63%, 63%, 63%, and 61% amino acid (aa) identity with rat, human, canine, porcine, and bovine PECAM-1, respectively. PECAM-1 participates with other adhesion molecules in some functions, but is the critical molecule for TEM. Homotypic PECAM-1 adhesion in trans, combined with cycling of PECAM-1 to and from surface-connected endothelial cell vesicles, leads leukocytes across endothelial tight junctions (3, 10). Homotypic adhesion and signaling functions also strongly suppress mitochondria-dependent apoptosis (11). In platelets, PECAM-1 is necessary for limiting thrombus formation (12) and promoting integrin-mediated clot retraction and platelet spreading (13), but mechanisms for these phenomena are unclear. PECAM-/- mice are deficient in chemokine-mediated chemotaxis (14).
- Ilan, N. and J.A. Madri (2003) Curr. Opin. Cell Biol. 15:515.
- Xie, Y. and W.A. Muller (1993) Proc. Natl. Acad. Sci. USA 90:5569.
- Liao, F. et al. (1997) J. Exp. Med. 185:1349.
- Nakada, M.T. et al. (2000) J. Immunol. 164:452.
- Chemnitz, J.M. et al. (2004) J. Immunol. 173:945.
- Ilan, N. et al. (2001) FASEB J. 15:362.
- Eugenin, E.A. et al. (2006) J. Leukoc. Biol. 79:444.
- Losy, J. et al. (1999) J. Neuroimmunol. 99:169.
- Wang, Y. et al. (2003) Am. J. Physiol. Heart Circ. Physiol. 284:H1008.
- Mamdouh, Z. et al. (2003) Nature 421:748.
- Gao, C. et al. (2003) Blood 102:169.
- Falati, S. et al. (2006) Blood 107:535.
- Wee, J.L. and D.E. Jackson (2005) Blood 106:3816.
- Wu, Y. et al. (2005) J. Immunol. 175:3484.
Product Datasheets
Citations for Mouse/Rat CD31/PECAM-1 APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Kruppel-like factor 3 inhibition by mutated lncRNA Reg1cp results in human high bone mass syndrome
Authors: Yang M, Guo Q, Peng H et al.
J. Exp. Med.
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Apelin modulates inflammation and leukocyte recruitment in experimental autoimmune encephalomyelitis
Authors: Park, H;Song, J;Jeong, HW;Grönloh, MLB;Koh, BI;Bovay, E;Kim, KP;Klotz, L;Thistlethwaite, PA;van Buul, JD;Sorokin, L;Adams, RH;
Nature communications
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Early Impairment of Paracrine and Phenotypic Features in Resident Cardiac Mesenchymal Stromal Cells after Thoracic Radiotherapy
Authors: Picchio, V;Gaetani, R;Pagano, F;Derevyanchuk, Y;Pagliarosi, O;Floris, E;Cozzolino, C;Bernava, G;Bordin, A;Rocha, F;Pereira, ARS;Ministro, A;Pinto, AT;De Falco, E;Serino, G;Massai, D;Tamarat, R;Pesce, M;Santos, SCR;Messina, E;Chimenti, I;
International journal of molecular sciences
Species: Rat
Sample Types: Whole Cells
Applications: Flow Cytometry -
miR-188-3p targets skeletal endothelium coupling of angiogenesis and osteogenesis during ageing
Authors: WZ He, M Yang, Y Jiang, C He, YC Sun, L Liu, M Huang, YR Jiao, KX Chen, J Hou, M Huang, YL Xu, X Feng, Y Liu, Q Guo, H Peng, Y Huang, T Su, Y Xiao, Y Li, C Zeng, G Lei, XH Luo, CJ Li
Cell Death & Disease, 2022-05-25;13(5):494.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Osteoclasts protect bone blood vessels against senescence through the angiogenin/plexin-B2 axis
Authors: X Liu, Y Chai, G Liu, W Su, Q Guo, X Lv, P Gao, B Yu, G Ferbeyre, X Cao, M Wan
Nature Communications, 2021-03-23;12(1):1832.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Ophiopogonin D promotes bone regeneration by stimulating CD31hi EMCNhi vessel formation
Authors: Mi Yang, Chang‐Jun Li, Ye Xiao, Qi Guo, Yan Huang, Tian Su et al.
Cell Proliferation
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MiR-497?195 cluster regulates angiogenesis during coupling with osteogenesis by maintaining endothelial Notch and HIF-1? activity
Authors: M Yang, CJ Li, X Sun, Q Guo, Y Xiao, T Su, ML Tu, H Peng, Q Lu, Q Liu, HB He, TJ Jiang, MX Lei, M Wan, X Cao, XH Luo
Nat Commun, 2017-07-07;8(0):16003.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Cell-matrix signals specify bone endothelial cells during developmental osteogenesis
Authors: UH Langen, ME Pitulescu, JM Kim, R Enriquez-G, KK Sivaraj, AP Kusumbe, A Singh, J Di Russo, MG Bixel, B Zhou, L Sorokin, JM Vaquerizas, RH Adams
Nat. Cell Biol, 2017-02-20;19(3):189-201.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Age-dependent modulation of vascular niches for haematopoietic stem cells
Authors: AP Kusumbe, SK Ramasamy, T Itkin, MA Mäe, UH Langen, C Betsholtz, T Lapidot, RH Adams
Nature, 2016-04-13;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Coupling of angiogenesis and osteogenesis by a specific vessel subtype in bone.
Authors: Kusumbe A, Ramasamy S, Adams R
Nature, 2014-03-12;507(7492):323-8.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Decreased blood vessel density and endothelial cell subset dynamics during ageing of the endocrine system
Authors: Chen J, Lippo L, Labella R et al.
Onco Targets Ther
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Average Rating: 5 (Based on 1 Review)
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Reason for Rating: Works excellent in the mouse bone marrow cells. Sample Tested: Bone marrow cells Species: Mouse
