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Mouse/Rat Apolipoprotein H/ApoH Antibody

Catalog #: AF6575
2 Citations Datasheet
Catalog # Availability Size / Price Qty
AF6575
AF6575-SP
Detection of Rat and Mouse Apolipoprotein H/ApoH by Western Blot.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse/Rat Apolipoprotein H/ApoH Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects mouse and rat Apolipoprotein H/ApoH in Western blots and detects recombinant mouse Apolipoprotein H/ApoH in direct ELISAs. In direct ELISAs, approximately 40% cross-reactivity with recombinant human Apolipoprotein H/ApoH is observed.  
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Apolipoprotein H/ApoH
Gly20-Cys345
Accession # Q01339
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Rat and Mouse Apolipoprotein H/ApoH antibody by Western Blot. View Larger

Detection of Rat and Mouse Apolipoprotein H/ApoH by Western Blot. Western blot shows lysates of rat liver tissue and mouse serum. PVDF membrane was probed with 0.25 µg/mL of Sheep Anti-Mouse/Rat Apolipoprotein H/ApoH Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6575) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Apolipoprotein H/ApoH at approximately 50-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Apolipoprotein H/ApoH

Apolipoprotein H (ApoH), also known as beta 2‑Glycoprotein I/ beta 2-GPI, is a variably glycosylated member of the complement control superfamily of proteins with a molecular weight of aproximately 50 kDa (1, 2). Mature mouse ApoH consists of four tandem Sushi/SCR repeats followed by one Sushi-like repeat (3, 4). Mature mouse ApoH shares 76% and 42% aa sequence identity with human and rat ApoH, respectively. Hepatocyte-derived ApoH binds directly to negatively charged phospholipids (5). It circulates as a component of lipoprotein particles and as a lipid-free serum protein (6). ApoH also associates with liposomes and apoptotic cell debris, thereby enabling their renal clearance via Megalin uptake (7, 8). Circulating levels of ApoH are postively correlated with triglyceride-rich lipoprotein (VLDL) components in type II diabetes (9). ApoH inhibits thrombosis by blocking the activation of Coagulation Factor XI but also shows procoagulant activity by inhibiting the activation of Protein C (10, 11). ApoH can be cleaved by Plasmin at Lys317‑Thr318, an action that is enhanced by heparin (12, 13). ApoH cleavage reduces its ability to bind phospholipids and inhibit Factor XI activation but confers the ability to bind Plasminogen (10, 12, 14). Cleaved ApoH also demonstrates antiangiogenic activity, whereas intact ApoH does not (14). The production of antibodies against ApoH is a hallmark of Antiphospholipid Syndrome (APS), an autoimmune disorder that leads to hypercoagulability and recurrent miscarriages (15). ApoH binds to the surface antigen of Hepatitis B Virus and is associated with the development of HBV-induced hepatocellular carcinoma (6, 16).

References
  1. Crook, M.A. et al. (2010) Atherosclerosis 209:32.
  2. Miyakis, S. et al. (2004) Thromb. Res. 114:335.
  3. Nonaka, M. et al. (1992) Genomics 13:1082.
  4. Lozier, J. et al. (1984) Proc. Natl. Acad. Sci. 81:3640.
  5. Wurm, H. (1984) Int. J. Biochem. 16:511.
  6. Mehdi, H. et al. (1994) J. Virol. 68:2415.
  7. Chonn, A. et al. (1995) J. Biol. Chem. 270:25845.
  8. Moestrup, S.K. et al. (1998) J. Clin. Invest. 102:902.
  9. Castro, A. et al. (2010) Atherosclerosis 209:201.
  10. Shi, T. et al. (2004) Proc. Natl. Acad. Sci. 101:3939.
  11. Mori, T. et al. (1996) Thromb. Haemost. 75:49.
  12. Hunt, J. et al. (1993) Proc. Natl. Acad. Sci. 90:2141.
  13. Guerin, J. et al. (2002) J. Biol. Chem. 277:2644.
  14. Sakai, T. et al. (2007) Am. J. Pathol. 171:1659.
  15. Adams, M. (2008) Semin. Thromb. Haemost. 34:251.
  16. Jing, X. et al. (2010) J. Cancer Res. Clin. Oncol. 16:1671.
Entrez Gene IDs
350 (Human); 11818 (Mouse); 287774 (Rat)
Alternate Names
Activated protein C-binding protein; Anticardiolipin cofactor; APC inhibitor; APOH; apo-H; apolipoprotein H (beta-2-glycoprotein I); Apolipoprotein H; B2G1; B2GP1; B2GPI; Beta; beta(2)GPI; beta-2-glycoprotein 1; Beta-2-glycoprotein I; BG

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Citations for Mouse/Rat Apolipoprotein H/ApoH Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Gram Negative Bacterial Inflammation Ameliorated by the Plasma Protein Beta 2-Glycoprotein I
    Sci Rep, 2016-09-27;6(0):33656.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Development (Capture)
  2. Cytosolic aldose metabolism contributes to progression from cirrhosis to hepatocarcinogenesis
    Authors: Z. Oaks, A. Patel, N. Huang, G. Choudhary, T. Winans, T. Faludi et al.
    Nature Metabolism

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