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Mouse Periostin/OSF-2 Isoform 2 Antibody

Catalog #: AF2955
30 Citations 6 Reviews Datasheet
Catalog # Availability Size / Price Qty
AF2955
AF2955-SP
Cell Adhesion Mediated by Periostin/OSF‑2 and Neutral-ization by Mouse Periostin/ OSF‑2 Antibody.
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Product Details
Citations (30)
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Supplemental Products
Reviews (6)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Periostin/OSF-2 Isoform 2 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Periostin/OSF‑2 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Periostin/OSF‑2
Asn24-Gln811
Accession # Q62009
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Periostin/OSF‑2 (Catalog # 2955-F2)
Immunocytochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize Periostin/OSF‑2-mediated adhesion of the ATDC5 mouse chondrogenic cell line. The Neutralization Dose (ND50) is typically 1-5 µg/mL in the presence of 5 µg/mL Recombinant Mouse Periostin/OSF‑2.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Adhesion Mediated by Periostin/OSF‑2 and Neutral-ization by Mouse Periostin/ OSF‑2 Antibody. View Larger

Cell Adhesion Mediated by Periostin/OSF‑2 and Neutral-ization by Mouse Periostin/ OSF‑2 Antibody. Recombinant Mouse Periostin/OSF-2 (2955-F2), immobilized onto a microplate, supports the adhesion of the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Mouse Periostin/OSF-2 (5 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse Periostin/ OSF-2 Isoform 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2955). The ND50 is typically 1-5 µg/mL.

Immunocytochemistry Periostin/OSF-2 antibody in Rat Mesenchymal Stem Cells by Immunocytochemistry (ICC). View Larger

Periostin/OSF‑2 in Rat Mesenchymal Stem Cells. Periostin/OSF-2 was detected in immersion fixed rat mesenchymal stem cells differentiated to osteoblasts using Goat Anti-Mouse Periostin/OSF-2 Isoform 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2955) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Periostin/OSF-2

Mouse Periostin, also known as OSF-2 (osteoblast-specific factor 2) is a 170 kDa, secreted, homodimeric protein that belongs to the periostin family of the FAS1 superfamily of molecules (1‑4). It is a TGF-beta inducible molecule that serves as both an adhesion molecule and tumor suppressor (2, 5, 6, 7). It is synthesized as a 838 amino acid (aa) precursor that contains a 23 aa signal sequence and an 815 aa mature region (2, 8). It is unknown if the molecule has any significant glycosylation (2). Based on human OSF-2, the homodimer is not disulfide-linked (3). The molecule consists of two distinct regions. The N-terminus contains an 55 aa EMI domain, while the C-terminus contains four, 130 aa fasciculin type 1 (or FAS1) domains. The EMI domain is cysteine-rich and shows a highly basic alpha -helix (9). Each FAS1 repeat exhibits a novel 7-stranded beta -wedge with a multiple alpha -helix fold (1, 8). Multiple alternate splice forms are known to exist C-terminal (aa 672‑812) to the four-fold FAS1 repeats. These mature molecules are 760, 761, 787 and 788 aa in length and show block deletions of 54 aa, 27 aa and/or 28 aa (10). The significance of the alternate splice forms is not clear. They do, however, appear to be temporally regulated (6). OSF-2 is known to bind to alpha v beta 3 and alpha v beta 5 integrins (3). It is synthesized by smooth muscle cells, fibroblasts and osteoblasts (2, 5, 7). Mature mouse OSF-2 shares 98%, 92% and 91% aa identity with rat, canine and human OSF‑2, respectively.

References
  1. Clout, N.J. and D. Tisi (2003) Structure 11:197.
  2. Horiuchi, K. et al. (1999) J. Bone Miner. Res. 14:1239.
  3. Gillan, L. et al. (2002) Cancer Res. 62:5358.
  4. Litvin, J. et al. (2005) Anat. Rec. A Discov. Mol. Cell. Evol. Biol. 287A:1205.
  5. Lindner, V. et al. (2005) Arterioscler. Thromb. Vasc. Biol. 25:77.
  6. Kruzynska-Frejtag, A. et al. (2004) Dev. Dyn. 229:857.
  7. Yoshioka, N. et al. (2002) Exp. Cell Res. 279:91.
  8. Takeshita, S. et al. (1993) Biochem. J. 294:271.
  9. Callebaut, I. et al. (2003) Biochem. Biophys. Res. Commun. 300:619.
  10. Swiss-Prot Accession # Q62009.
Long Name
Osteoblast Specific Factor 2
Entrez Gene IDs
10631 (Human); 50706 (Mouse); 361945 (Rat)
Alternate Names
Fasciclin I-like; MGC119510; MGC119511; OSF2; OSF-2; OSF-2osteoblast specific factor 2 (fasciclin I-like); OSF2periodontal ligament-specific periostin; Osteoblast-specific factor 2; PDLPOSTN; periostin isoform thy2; periostin isoform thy4; periostin isoform thy6; periostin isoform thy8; Periostin; periostin, osteoblast specific factor; PNRP11-412K4.1; POSTN; TRIF52

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Citations for Mouse Periostin/OSF-2 Isoform 2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

30 Citations: Showing 1 - 10
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  1. Matrix metalloproteinase 14 is required for fibrous tissue expansion
    Authors: Susan H Taylor, Ching-Yan Chloé Yeung, Nicholas S Kalson, Yinhui Lu, Paola Zigrino, Tobias Starborg et al.
    eLife
  2. Early Cardiac Remodeling Promotes Tumor Growth and Metastasis
    Authors: Shimrit Avraham, Soraya Abu-Sharki, Rona Shofti, Tali Haas, Ben Korin, Roy Kalfon et al.
    Circulation
  3. Association of Differentially Altered Liver Fibrosis with Deposition of TGFBi in Stabilin-Deficient Mice
    Authors: Jessica Krzistetzko, Cyrill Géraud, Christof Dormann, Anna Riedel, Thomas Leibing
    International Journal of Molecular Sciences
  4. Alendronate partially rescues the periodontal defects in OIM mouse model of osteogenesis imperfecta
    Authors: Chen, P;Wang, K;Lin, M;Sharma, R;Kalajzi?, Ž;O’Brien, M;Yadav, S;
    Scientific reports
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  5. Single-nucleus transcriptomics reveal the differentiation trajectories of periosteal skeletal/stem progenitor cells in bone regeneration
    Authors: Perrin, S;Ethel, M;Bretegnier, V;Goachet, C;Wotawa, CA;Luka, M;Coulpier, F;Masson, C;Ménager, M;Colnot, C;
    eLife
    Species: Mouse, Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  6. Single nuclei transcriptomics reveal the differentiation trajectories of periosteal skeletal/stem progenitor cells in bone regeneration
    Authors: Perrin, S;Ethel, M;Bretegnier, V;Goachet, C;Wotawa, CA;Luka, M;Coulpier, F;Masson, C;Ménager, M;Colnot, C;
    bioRxiv : the preprint server for biology
    Species: Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  7. Phenotypical and biochemical characterization of murine psoriasiform and fibrotic skin disease models in Stabilin-deficient mice
    Authors: Krzistetzko, J;Géraud, C;Dormann, C;Riedel, A;Leibing, T;
    FEBS open bio
    Species: Mouse, Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  8. Deficiency of Stabilin-1 in the Context of Hepatic Melanoma Metastasis
    Authors: Wohlfeil, SA;Olsavszky, A;Irkens, AL;Häfele, V;Dietsch, B;Straub, N;Goerdt, S;Géraud, C;
    Cancers
    Species: Mouse, Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. Lgr5-expressing secretory cells form a Wnt inhibitory niche in cartilage critical for chondrocyte identity
    Authors: Ruscitto, A;Chen, P;Tosa, I;Wang, Z;Zhou, G;Safina, I;Wei, R;Morel, MM;Koch, A;Forman, M;Reeve, G;Lecholop, MK;Wilson, M;Bonthius, D;Chen, M;Ono, M;Wang, TC;Yao, H;Embree, MC;
    Cell stem cell
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. Association of Differentially Altered Liver Fibrosis with Deposition of TGFBi in Stabilin-Deficient Mice
    Authors: Jessica Krzistetzko; Cyrill Géraud; Christof Dormann; Anna Riedel; Thomas Leibing
    International Journal of Molecular Sciences
    Species: Transgenic Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: Immunohistochemistry, Simple Western
  11. Diabetes Induces Cardiac Fibroblast Activation, Promoting a Matrix-Preserving Nonmyofibroblast Phenotype, Without Stimulating Pericyte to Fibroblast Conversion
    Authors: L Alex, I Tuleta, A Hanna, NG Frangogian
    Journal of the American Heart Association, 2023-03-09;0(0):e027463.
    Species: Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  12. Periosteal skeletal stem cells can migrate into the bone marrow and support hematopoiesis after injury
    Authors: Marchand, T;Akinnola, KE;Takeishi, S;Maryanovich, M;Pinho, S;Saint-Vanne, J;Birbrair, A;Lamy, T;Tarte, K;Frenette, PS;Gritsman, K;
    bioRxiv : the preprint server for biology
    Species: Transgenic Mouse, Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  13. Bone marrow and periosteal skeletal stem/progenitor cells make distinct contributions to bone maintenance and repair
    Authors: EC Jeffery, TLA Mann, JA Pool, Z Zhao, SJ Morrison
    Cell Stem Cell, 2022-10-21;29(11):1547-1561.e6.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  14. FGFR3 in Periosteal Cells Drives Cartilage-to-Bone Transformation in Bone Repair
    Authors: A Julien, S Perrin, O Duchamp de, C Carvalho, M Bensidhoum, L Legeai-Mal, C Colnot
    Stem Cell Reports, 2020-09-10;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  15. Hox11 expressing regional skeletal stem cells are progenitors for osteoblasts, chondrocytes and adipocytes throughout life
    Authors: KM Pineault, JY Song, KM Kozloff, D Lucas, DM Wellik
    Nat Commun, 2019-07-18;10(1):3168.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  16. Short-term RANKL exposure initiates a neoplastic transcriptional program in the basal epithelium of the murine salivary gland
    Authors: L Hai, MM Szwarc, DM Lonard, K Rajapakshe, D Perera, C Coarfa, M Ittmann, R Fernandez-, JP Lydon
    Cytokine, 2019-06-18;123(0):154745.
    Species: Human
    Sample Types: Tissue Lysates, Whole Tissue
    Applications: IHC, Western Blot
  17. New Noonan syndrome model mice with RIT1 mutation exhibit cardiac hypertrophy and susceptibility to ?-adrenergic stimulation-induced cardiac fibrosis
    Authors: S Takahara, SI Inoue, S Miyagawa-T, K Matsuura, Y Nakashima, T Niihori, Y Matsubara, Y Saiki, Y Aoki
    EBioMedicine, 2019-03-18;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  18. Periostin deletion suppresses late-phase response in mouse experimental allergic conjunctivitis
    Authors: Y Asada, M Okano, W Ishida, S Iwamoto, K Fukuda, T Hirakata, N Tada, A Fukushima, N Ebihara, A Kudo, A Matsuda
    Allergol Int, 2018-11-09;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  19. Macrophage-derived granulin drives resistance to immune checkpoint inhibition in metastatic pancreatic cancer
    Authors: V Quaranta, C Rainer, SR Nielsen, ML Raymant, MS Ahmed, DD Engle, A Taylor, T Murray, F Campbell, DH Palmer, DA Tuveson, A Mielgo, MC Schmid
    Cancer Res., 2018-05-22;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  20. Multicolor quantitative confocal imaging cytometry
    Authors: DL Coutu, KD Kokkaliari, L Kunz, T Schroeder
    Nat. Methods, 2017-11-13;15(1):39-46.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  21. Deletion of the Ste20-like kinase SLK in skeletal muscle results in a progressive myopathy and muscle weakness
    Authors: BR Pryce, KN Al-Zahrani, S Dufresne, N Belkina, C Labr?che, G Patino-Lop, J Frenette, S Shaw, LA Sabourin
    Skelet Muscle, 2017-02-02;7(1):3.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  22. Cathepsin A inhibition attenuates myocardial infarction-induced heart failure on the functional and proteomic levels
    Authors: Agnese Petrera, Johann Gassenhuber, Sven Ruf, Deepika Gunasekaran, Jennifer Esser, Jasmin Hasmik Shahinian et al.
    Journal of Translational Medicine
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  23. Macrophage-secreted granulin supports pancreatic cancer metastasis by inducing liver fibrosis
    Authors: SR Nielsen, V Quaranta, A Linford, P Emeagi, C Rainer, A Santos, L Ireland, T Sakai, K Sakai, YS Kim, D Engle, F Campbell, D Palmer, JH Ko, DA Tuveson, E Hirsch, A Mielgo, MC Schmid
    Nat Cell Biol, 2016-04-18;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  24. A mouse model for dominant collagen VI disorders: heterozygous deletion of Col6a3 Exon 16.
    Authors: Pan T, Zhang R, Arita M, Bogdanovich S, Adams S, Gara S, Wagener R, Khurana T, Birk D, Chu M
    J Biol Chem, 2014-02-22;289(15):10293-307.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  25. Periostin links mechanical strain to inflammation in abdominal aortic aneurysm.
    Authors: Yamashita, Osamu, Yoshimura, Koichi, Nagasawa, Ayako, Ueda, Koshiro, Morikage, Noriyasu, Ikeda, Yasuhiro, Hamano, Kimikazu
    PLoS ONE, 2013-11-19;8(11):e79753.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Neutralization
  26. Pressure overload-induced cardiac remodeling and dysfunction in the absence of interleukin 6 in mice.
    Authors: Lai, N Chin, Gao, Mei Hua, Tang, Eric, Tang, Ruoying, Guo, Tracy, Dalton, Nancy D, Deng, Aihua, Tang, Tong
    Lab Invest, 2012-07-23;92(11):1518-26.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  27. Quantitative analysis of the secretome of TGF-beta signaling-deficient mammary fibroblasts.
    Authors: Xu BJ, Yan W, Jovanovic B
    Proteomics, 2010-07-01;10(13):2458-70.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  28. Periostin, a member of a novel family of vitamin K-dependent proteins, is expressed by mesenchymal stromal cells.
    Authors: Coutu DL, Wu JH, Monette A, Rivard GE, Blostein MD, Galipeau J
    J. Biol. Chem., 2008-04-30;283(26):17991-8001.
    Species: Human, Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  29. Cathepsin A inhibition attenuates myocardial infarction-induced heart failure on the functional and proteomic levels
    Authors: Agnese Petrera, Johann Gassenhuber, Sven Ruf, Deepika Gunasekaran, Jennifer Esser, Jasmin Hasmik Shahinian et al.
    Journal of Translational Medicine
  30. Periostin gene expression in neu-positive breast cancer cells is regulated by a FGFR signaling cross talk with TGF beta /PI3K/AKT pathways
    Authors: Cédrik Labrèche, David P. Cook, John Abou-Hamad, Julia Pascoal, Benjamin R. Pryce, Khalid N. Al-Zahrani et al.
    Breast Cancer Research

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Reviews for Mouse Periostin/OSF-2 Isoform 2 Antibody

Average Rating: 4.7 (Based on 6 Reviews)

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Mouse Periostin/OSF-2 Isoform 2 Antibody
By Anonymous on 07/19/2021
Application: WB Sample Tested: Heart tissue Species: Mouse
Western Blot Mouse Periostin/OSF-2 Isoform 2 Antibody AF2955
Mouse Periostin/OSF-2 Isoform 2 Antibody
By Anonymous on 06/29/2021
Application: IHC Sample Tested: Bladder tissue Species: Rat
Immunohistochemistry Mouse Periostin/OSF-2 Isoform 2 Antibody AF2955
Mouse Periostin/OSF-2 Isoform 2 Antibody
By Anonymous on 05/10/2020
Application: WB Sample Tested: Adult heart Species: Mouse
Western Blot Mouse Periostin/OSF-2 Isoform 2 Antibody AF2955
Mouse Periostin/OSF-2 Isoform 2 Antibody
By Anonymous on 05/10/2020
Application: Immunocytochemistry/Immunofluorescence Sample Tested: primary cardiac fibroblasts Species: Rat
Immunocytochemistry/Immunofluorescence Mouse Periostin/OSF-2 Isoform 2 Antibody AF2955
Mouse Periostin/OSF-2 Isoform 2 Antibody
By Anonymous on 05/10/2020
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Adult heart Species: Mouse
Immunocytochemistry/Immunofluorescence Mouse Periostin/OSF-2 Isoform 2 Antibody AF2955
Mouse Periostin/OSF-2 Isoform 2 Antibody
By Anonymous on 06/17/2019
Application: WB Sample Tested: Tissue Lysates Species: Mouse
Western Blot Mouse Periostin/OSF-2 Isoform 2 Antibody AF2955