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Mouse PD-1 Antibody

Catalog #: AF1021
24 Citations 4 Reviews Datasheet
Catalog # Availability Size / Price Qty
AF1021
AF1021-SP
Detection of Mouse PD‑1 by Western Blot.
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Product Details
Citations (24)
FAQs
Supplemental Products
Reviews (4)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse PD-1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse PD-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human PD‑1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse PD-1
Leu25-Gln167
Accession # Q02242
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
Mouse splenocytes treated with PHA
Immunohistochemistry
5-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse PD-1 antibody by Western Blot. View Larger

Detection of Mouse PD‑1 by Western Blot. Western blot shows lysates of EL-4 mouse lymphoblast cell line and CTLL-2 mouse cytotoxic T cell line (negative control). PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse PD-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1021) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PD-1 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Detection of Mouse PD-1 antibody by Western Blot. View Larger

Detection of Mouse PD‑1 by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line mock transfected or transfected with mouse PD-1. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse PD-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1021) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PD-1 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry PD‑1 antibody in Mouse Thymus by Immunohistochemistry (IHC-Fr). View Larger

PD‑1 in Mouse Thymus. PD‑1 was detected in perfusion fixed frozen sections of mouse thymus using 15 µg/mL Mouse PD‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1021) overnight at 4 °C. Tissue was stained (red) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Immunohistochemistry PD-1 antibody in Mouse Spleen by Immunohistochemistry (IHC-Fr). View Larger

PD‑1 in Mouse Spleen. PD-1 was detected in perfusion fixed frozen sections of mouse spleen using Goat Anti-Mouse PD-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1021) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in splenocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Immunohistochemistry PD-1 antibody in Mouse Thymus by Immunohistochemistry (IHC-P). View Larger

PD‑1 in Mouse Thymus. PD-1 was detected in perfusion fixed paraffin-embedded sections of mouse thymus using Goat Anti-Mouse PD-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1021) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Western Blot Detection of Mouse PD-1 by Western Blot View Larger

Detection of Mouse PD-1 by Western Blot Expression and glycosylation of Siglec-1 counter-receptors on resting and activated Tregs.(A) Eight Siglec-1 counter receptors were randomly selected for cytometry analysis. All experiments were performed three times and similar results were observed. (B) Normalized mRNA counts of Siglec-1 counter receptors were obtained from Treg RNA-Seq data. The result is visualized in a volcano plot. Only a small subset of counter-receptors showed strongly increased gene expression following Treg activation. The counter-receptors selected for flow cytometry analysis are highlighted in red. (C) Western blot showing that CD48 had a higher molecular weight in activated Tregs. (D) Western blot of PD-1 affinity-purified from resting and activated Tregs. PD 1 showed a dramatic decrease of molecular weight after PNGase F digestion (D), PD-1 from activated Tregs had higher molecular weight (E) and following sialidase treatment, PD-1 from resting and activated Tregs migrated at a slightly reduced molecular weight indicating the presence of sialic acids (F). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35224210), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse PD-1 by Western Blot View Larger

Detection of Mouse PD-1 by Western Blot Expression and glycosylation of Siglec-1 counter-receptors on resting and activated Tregs.(A) Eight Siglec-1 counter receptors were randomly selected for cytometry analysis. All experiments were performed three times and similar results were observed. (B) Normalized mRNA counts of Siglec-1 counter receptors were obtained from Treg RNA-Seq data. The result is visualized in a volcano plot. Only a small subset of counter-receptors showed strongly increased gene expression following Treg activation. The counter-receptors selected for flow cytometry analysis are highlighted in red. (C) Western blot showing that CD48 had a higher molecular weight in activated Tregs. (D) Western blot of PD-1 affinity-purified from resting and activated Tregs. PD 1 showed a dramatic decrease of molecular weight after PNGase F digestion (D), PD-1 from activated Tregs had higher molecular weight (E) and following sialidase treatment, PD-1 from resting and activated Tregs migrated at a slightly reduced molecular weight indicating the presence of sialic acids (F). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35224210), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse PD-1 by Western Blot View Larger

Detection of Mouse PD-1 by Western Blot Expression and glycosylation of Siglec-1 counter-receptors on resting and activated Tregs.(A) Eight Siglec-1 counter receptors were randomly selected for cytometry analysis. All experiments were performed three times and similar results were observed. (B) Normalized mRNA counts of Siglec-1 counter receptors were obtained from Treg RNA-Seq data. The result is visualized in a volcano plot. Only a small subset of counter-receptors showed strongly increased gene expression following Treg activation. The counter-receptors selected for flow cytometry analysis are highlighted in red. (C) Western blot showing that CD48 had a higher molecular weight in activated Tregs. (D) Western blot of PD-1 affinity-purified from resting and activated Tregs. PD 1 showed a dramatic decrease of molecular weight after PNGase F digestion (D), PD-1 from activated Tregs had higher molecular weight (E) and following sialidase treatment, PD-1 from resting and activated Tregs migrated at a slightly reduced molecular weight indicating the presence of sialic acids (F). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35224210), licensed under a CC-BY license. Not internally tested by R&D Systems.

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PD-1

Programmed Death-1 (PD-1) is type I transmembrane protein belonging to the CD28/CTLA-4 family of immunoreceptors that mediate signals for regulating immune responses (1). Other members of this family include CD28, CTLA-4, and ICOS (2-4). PD-1 is most closely related to CTLA-4 and shares approximately 24% amino acid (aa) sequence identity. The mouse PD-1 gene encodes a 288 aa protein with a putative 20 aa signal peptide, a 149 aa extracellular region with one immunoglobulin-like V-type domain, a 21 aa transmembrane domain, and a 98 aa cytoplasmic region. The cytoplasmic tail contains two tyrosine residues that form the immunoreceptor tyrosine-based inhibitory motif (ITIM) and immunoreceptor tyrosine-based switch motif (ITSM) that are important in mediating PD-1 signaling. Mouse and human PD-1 share approximately 69% aa sequence identity. Two B7 family proteins, PD-L1 (also called B7-H1) and PD-L2, have been identified as PD-1 ligands (5, 6). PD-1 is expressed on activated T cells, B cells, myeloid cells, and on a subset of thymocytes. PD-1 deficient mice have a defect in peripheral tolerance and spontaneously develop autoimmune diseases. Binding of PD-1 to PD-L1 or PD-L2 results in the inhibition of TCR-mediated proliferation and cytokine production as well as BCR-mediated signaling. PD-1 likely has an inhibitory role in regulating immune responses (1-4).

References
  1. Ishida, Y. et al. (1992) EMBO J. 11:3887.
  2. Sharpe, A.H. and G.J. Freeman (2002) Nat. Rev. Immunol. 2:116.
  3. Coyle, A. and J. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
  4. Nishimura, H. and T. Honjo (2001) Trends in Immunol. 22:265.
  5. Latchman Y. et al. (2001) Nature Immun. 2:261.
  6. Tamura, H. et al. (2001) Blood 97:1809.
Long Name
Programmed Death-1
Entrez Gene IDs
5133 (Human); 18566 (Mouse); 301626 (Rat); 100533201 (Porcine); 486213 (Canine); 102123659 (Cynomolgus Monkey)
Alternate Names
CD279 antigen; CD279; hPD-1; PD1; PD-1; PD1hPD-l; PDCD1; programmed cell death 1; programmed cell death protein 1; Protein PD-1; SLEB2

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Citations for Mouse PD-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

24 Citations: Showing 1 - 10
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  1. Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes.
    Authors: Lin J. R, Izar B, et al.
    Elife
  2. Orthogonal cytokine engineering enables novel synthetic effector states escaping canonical exhaustion in tumor-rejecting CD8+ T cells
    Authors: Corria-Osorio J, Carmona SJ, Stefanidis E, Andreatta M, Ortiz-Miranda Y, Muller T, Rota IA, Crespo I, Seijo B, Castro W, Jimenez-Luna C, Scarpellino L, Ronet C, Spill A, Lanitis E, Romero P, Luther SA, Irving M, Coukos G
    Nat Immunol. 2023; 24(5): 869–883.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Capture
  3. Mice transgenic for human CTLA4-CD28 fusion gene show proliferation and transformation of ATLL-like and AITL-like T cells
    Authors: Gyu Jin Lee, Yukyung Jun, Yoon Kyung Jeon, Daekee Lee, Sanghyuk Lee, Jaesang Kim
    OncoImmunology
  4. CD8+ T cells induce interferon-responsive oligodendrocytes and microglia in white matter aging
    Authors: T Kaya, N Mattugini, L Liu, H Ji, L Cantuti-Ca, J Wu, M Schifferer, J Groh, R Martini, S Besson-Gir, S Kaji, A Liesz, O Gokce, M Simons
    Nature Neuroscience, 2022-10-24;25(11):1446-1457.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. PD-1-cis IL-2R agonism yields better effectors from stem-like CD8+ T cells
    Authors: L Codarri De, V Nicolini, M Hashimoto, M Karagianni, PC Schwalie, L Lauener, EM Varypataki, M Richard, E Bommer, J Sam, S Joller, M Perro, F Cremasco, L Kunz, E Yanguez, T Hüsser, R Schlenker, M Mariani, V Tosevski, S Herter, M Bacac, I Waldhauer, S Colombetti, X Gueripel, S Wullschleg, M Tichet, D Hanahan, HT Kissick, S Leclair, A Freimoser-, S Seeber, V Teichgräbe, R Ahmed, C Klein, P Umaña
    Nature, 2022-09-28;610(7930):161-172.
    Species: Xenograft
    Sample Types: Whole Tissue
    Applications: IHC
  6. Distinct antibody clones detect PD-1 checkpoint expression and block PD-L1 interactions on live murine melanoma cells
    Authors: C Martins, M Silva, E Rasbach, P Singh, Y Itoh, JB Williams, E Statham, A Meurer, DV Martinez, A Brandenbur, MV Heppt, SR Barthel, T Schatton
    Scientific Reports, 2022-07-21;12(1):12491.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. AXL Inhibition in Macrophages Stimulates Host-versus-Leukemia Immunity and Eradicates Naïve and Treatment-Resistant Leukemia
    Authors: Irene Tirado-Gonzalez, Arnaud Descot, Devona Soetopo, Aleksandra Nevmerzhitskaya, Alexander Schäffer, Ivan-Maximilano Kur et al.
    Cancer Discovery
  8. Activation of regulatory T cells triggers specific changes in glycosylation associated with Siglec-1-dependent inflammatory responses
    Authors: Gang Wu, Gavuthami Murugesan, Manjula Nagala, Alex McCraw, Stuart M. Haslam, Anne Dell et al.
    Wellcome Open Research
  9. Eliminating mesothelioma by AAV-vectored, PD1-based vaccination in the tumor microenvironment
    Authors: Zhiwu Tan, Mei Sum Chiu, Chi Wing Yan, Kwan Man, Zhiwei Chen
    Molecular Therapy - Oncolytics
  10. Auto-aggressive CXCR6+ CD8 T cells cause liver immune pathology in NASH
    Authors: M Dudek, D Pfister, S Donakonda, P Filpe, A Schneider, M Laschinger, D Hartmann, N Hüser, P Meiser, F Bayerl, D Inverso, J Wigger, M Sebode, R Öllinger, R Rad, S Hegenbarth, M Anton, A Guillot, A Bowman, D Heide, F Müller, P Ramadori, V Leone, C Garcia-Cac, T Gruber, G Seifert, AM Kabat, JP Malm, S Reider, M Effenberge, S Roth, AT Billeter, B Müller-Sti, EJ Pearce, F Koch-Nolte, R Käser, H Tilg, R Thimme, T Böttler, F Tacke, JF Dufour, D Haller, PJ Murray, R Heeren, D Zehn, JP Böttcher, M Heikenwäld, PA Knolle
    Nature, 2021-03-24;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. Inhibitory Receptor Trap: A Platform for Discovery of Inhibitory Receptors That Utilize Inositol Lipid and Phosphotyrosine Phosphatase Effectors
    Authors: Bergren W. Crute, Rachel Sheraden, Vanessa L. Ott, Isaac T. W. Harley, Andrew Getahun, John C. Cambier
    Frontiers in Immunology
  12. Inhibition of TRPV1 by SHP-1 in nociceptive primary sensory neurons is critical in PD-L1 analgesia
    Authors: Ben-Long Liu, Qi-Lai Cao, Xin Zhao, Hui-Zhu Liu, Yu-Qiu Zhang
    JCI Insight
  13. Angioimmunoblastic T-cell lymphoma-like lymphadenopathy in mice transgenic for human RHOA with p.Gly17Val mutation
    Authors: Gyu Jin Lee, Yukyung Jun, Hae Yong Yoo, Yoon Kyung Jeon, Daekee Lee, Sanghyuk Lee et al.
    OncoImmunology
  14. Anti–PD-1 Induces M1 Polarization in the Glioma Microenvironment and Exerts Therapeutic Efficacy in the Absence of CD8 Cytotoxic T Cells
    Authors: Ganesh Rao, Khatri Latha, Martina Ott, Aria Sabbagh, Anantha Marisetty, Xiaoyang Ling et al.
    Clinical Cancer Research
  15. CD47 prevents the elimination of diseased fibroblasts in scleroderma
    Authors: T Lerbs, L Cui, ME King, T Chai, C Muscat, L Chung, R Brown, K Rieger, T Shibata, G Wernig
    JCI Insight, 2020-08-20;5(16):.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC
  16. HIF-1alpha and HIF-2alpha differently regulate tumour development and inflammation of clear cell renal cell carcinoma in mice
    Authors: R Hoefflin, S Harlander, S Schäfer, P Metzger, F Kuo, D Schönenber, M Adlesic, A Peighambar, P Seidel, CY Chen, M Consenza-C, A Jud, B Lahrmann, N Grabe, D Heide, FM Uhl, TA Chan, J Duyster, R Zeiser, C Schell, M Heikenwald, O Schilling, AA Hakimi, M Boerries, IJ Frew
    Nat Commun, 2020-08-17;11(1):4111.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  17. Bcl-6-directed follicular helper T cells promote vascular inflammatory injury in diabetic retinopathy
    Authors: Yan Liu, Ziqi Yang, Peilong Lai, Zijing Huang, Xiaowei Sun, Tian Zhou et al.
    Theranostics
  18. IL-17C-mediated innate inflammation decreases the response to PD-1 blockade in a model of Kras-driven lung cancer
    Authors: F Ritzmann, C Jungnickel, G Vella, A Kamyschnik, C Herr, D Li, MM Menger, A Angenendt, M Hoth, A Lis, R Bals, C Beisswenge
    Sci Rep, 2019-07-17;9(1):10353.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  19. Expression of lymphocyte-activating gene 3 and T-cell immunoreceptor with immunoglobulin and ITIM domains in cutaneous melanoma and their correlation with programmed cell death 1 expression in tumor-infiltrating lymphocytes
    Authors: WJ Lee, YJ Lee, ME Choi, KA Yun, CH Won, MW Lee, JH Choi, SE Chang
    J. Am. Acad. Dermatol., 2019-03-14;81(1):219-227.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  20. CD8?? intraepithelial lymphocytes arise from two main thymic precursors
    Authors: R Ruscher, RL Kummer, YJ Lee, SC Jameson, KA Hogquist
    Nat. Immunol., 2017-05-22;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  21. The development of endometrial hyperplasia in aged PD-1-deficient female mice
    Authors: Guoning Guo, Hong Li, Dayan Cao, Yongwen Chen
    Diagnostic Pathology
  22. Anti-programmed cell death 1 antibody reduces CD4+PD-1+ T cells and relieves the lupus-like nephritis of NZB/W F1 mice.
    Authors: Kasagi S, Kawano S, Okazaki T, Honjo T, Morinobu A, Hatachi S, Shimatani K, Tanaka Y, Minato N, Kumagai S
    J. Immunol., 2010-02-05;184(5):2337-47.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  23. Depletion of the programmed death-1 receptor completely reverses established clonal anergy in CD4(+) T lymphocytes via an interleukin-2-dependent mechanism.
    Authors: Bishop KD, Harris JE, Mordes JP, Greiner DL, Rossini AA, Czech MP, Phillips NE
    Cell. Immunol., 2009-02-23;256(1):86-91.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  24. IL-10 release upon PD-1 blockade sustains immunosuppression in ovarian cancer
    Authors: P Lamichhane, L Karyampudi, B Shreeder, J Krempski, D Bahr, J Daum, KR Kalli, EL Goode, MS Block, MJ Cannon, KL Knutson
    Cancer Res., 2017-10-09;0(0):.

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By Efstathios Stamatiades on 07/09/2019
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Mammary gland tissue Species: Mouse
Immunocytochemistry/Immunofluorescence Mouse PD-1 Antibody AF1021
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By Anonymous on 03/07/2018
Application: Func Sample Tested: mouse antibody Species: Mouse
Mouse PD-1 Antibody
By Anonymous on 09/29/2017
Application: ELISA Sample Tested: Human recombinant antibody Species: Mouse