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Mouse MSPR/Ron Antibody

Catalog #: AF431
3 Citations Datasheet
Catalog # Availability Size / Price Qty
AF431
AF431-SP
Chemotaxis Induced by MSP and Neutralization by Mouse MSP R/Ron Antibody.
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Citations (3)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse MSPR/Ron Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse MSP R/Ron in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human MSP R/Ron is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse MSP R/Ron
Ser24-Arg960 (Ile372Leu)
Accession # CAA52754
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse MSP R/Ron Fc Chimera (Catalog # 431-MS)
Neutralization
Measured by its ability to neutralize MSP-induced chemotaxis in mouse peritoneal macrophages [Wang, M-H. et al. (1994) J. Biol. Chem. 269:3436]. The Neutralization Dose (ND50) is typically 0.05-0.2 µg/mL in the presence of 50 ng/mL Recombinant Human MSP.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Chemotaxis Induced by MSP and Neutralization by Mouse MSP R/Ron Antibody. View Larger

Chemotaxis Induced by MSP and Neutralization by Mouse MSP R/Ron Antibody. Recombinant Human MSP (Catalog # 352-MS) chemoattracts mouse peritoneal macrophages in a dose-dependent manner (orange line). The amount of cells that migrated through filter was measured by LeukoStat™ staining (Fisher Scientific). Chemotaxis elicited by Recombinant Human MSP (50 ng/mL) is neutralized (green line) by increasing concentrations of Mouse MSP R/Ron Antigen Affinity-purified Polyclonal Antibody (Catalog # AF431). The ND50 is typically 0.05-0.2 µg/mL.

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MSPR/Ron

Macrophage stimulating protein receptor (MSP R), encoded by the human RON and the mouse Stk, is one of a small family of receptor tyrosine kinases (RTKs) that also includes human Met (the receptor for hepatocyte growth factor) and chicken Sea. This family of receptors is synthesized as a single-chain precursor that is cleaved into a mature disulfide-linked heterodimer composed of an extracellular alpha chain and a membrane spanning beta chain with intrinsic tyrosine kinase activity. Mouse MSP R cDNA encodes a 1378 amino acid (aa) residue presursor protein with a 23 aa signal peptide, a 287 aa residue alpha chain (Ser 24 - Arg 310) and a 1068 aa residue transmembrane beta chain (Gly 311 - Thr 1378). Expression of MSP receptor is restricted to specific areas of the central and peripheral nervous systems, epithelial cells along the digestive tract, skin and lung, and in subpopulations of the mononuclear phagocyte lineage. Both the heterodimeric MSP and the free MSP beta chain have been shown to bind to MSP R. However, only the heterodimeric MSP binding can induce receptor dimerization and phosphorylation and cause biological activity.

References
  1. Gaudino, G. et al. (1994) EMBO J. 13:3524.
  2. Wang, M-H. et al. (1994) Science 266:117.
  3. Wang, M-H. et al. (1997) J. Biol. Chem. 272:16999.
Long Name
Macrophage Stimulating Protein Receptor
Entrez Gene IDs
4486 (Human); 19882 (Mouse)
Alternate Names
CD136 antigen; CD136; CDw136c-met-related tyrosine kinase; EC 2.7.10; EC 2.7.10.1; macrophage stimulating 1 receptor (c-met-related tyrosine kinase); MSP R; MSP receptor; MSPR; MST1R; p185-Ron; Protein-tyrosine kinase 8; PTK8 protein tyrosine kinase 8; PTK8; Ron; RONmacrophage-stimulating protein receptor; soluble RON variant 1; soluble RON variant 2; soluble RON variant 3; soluble RON variant 4

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Citations for Mouse MSPR/Ron Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. The Ron Receptor Regulates Kupffer Cell-Dependent Cytokine Production and Hepatocyte Survival Following Endotoxin Exposure in Mice
    Authors: William D. Stuart, Rishikesh M. Kulkarni, Jerilyn K. Gray, Juozas Vasiliauskas, Mike A. Leonis, Susan E. Waltz
    Hepatology
  2. Neuroprotective Role of the Ron Receptor Tyrosine Kinase Underlying Central Nervous System Inflammation in Health and Disease
    Authors: A Dey, JN Allen, JW Fraser, LM Snyder, Y Tian, L Zhang, RF Paulson, A Patterson, MT Cantorna, PA Hankey-Gib
    Front Immunol, 2018-03-19;9(0):513.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Functional consequences of the macrophage stimulating protein 689C inflammatory bowel disease risk allele.
    Authors: Kauder, Steven E, Santell, Lydia, Mai, Elaine, Wright, Lilyan Y, Luis, Elizabet, N'Diaye, Elsa N, Lutman, Jeff, Ratti, Navneet, Sa, Susan M, Maun, Henry R, Stefanich, Eric, Gonzalez, Lino C, Graham, Robert R, Diehl, Lauri, Faubion, William, Keir, Mary E, Young, Judy, Chaudhuri, Amitabha, Lazarus, Robert A, Egen, Jackson
    PLoS ONE, 2013-12-23;8(12):e83958.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P

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