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Mouse Mer Antibody

Catalog #: MAB591
5 Citations Datasheet
Catalog # Availability Size / Price Qty
MAB591-SP
MAB591-500
MAB591-100
Mer in J774A.1 Mouse Cell Line.
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Product Details
Citations (5)
FAQs
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Mer Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Mer in direct ELISAs and Western blots. Does not cross-react with recombinant human Mer, recombinant mouse (rm) Axl, or rmDtk.
Source
Monoclonal Rat IgG1 Clone # 108921
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Mer
Glu23-Phe498
Accession # Q60805
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse Mer Fc Chimera (Catalog # 591-MR)
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Mer antibody in J774A by Immunocytochemistry (ICC).1 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

Mer in J774A.1 Mouse Cell Line. Mer was detected in immersion fixed J774A.1 mouse reticulum cell sarcoma macrophage cell line using Rat Anti-Mouse Mer Monoclonal Antibody (Catalog # MAB591) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Mer

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt) and Mer (human and mouse homologues of chicken c-Eyk) constitute a receptor tyrosine kinase subfamily. The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. These receptors bind the vitamin K-dependent protein growth-arrest-specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration or the prevention of apoptosis. Studies suggest that this family of tyrosine kinase receptors may be involved in hematopoiesis, embryonic development, tumorigenesis and regulation of testicular functions (1-2).

References
  1. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
  2. Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.
Long Name
Receptor Tyrosine Protein Kinase Mer
Entrez Gene IDs
10461 (Human); 17289 (Mouse); 102143798 (Cynomolgus Monkey)
Alternate Names
c-Eyk; c-mer proto-oncogene tyrosine kinase; C-mer; EC 2.7.10; EC 2.7.10.1; MER receptor tyrosine kinase; Mer; MerTK; MGC133349; Receptor tyrosine kinase MerTK; RP38Proto-oncogene c-Mer; STK kinase; tyrosine-protein kinase Mer

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Citations for Mouse Mer Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Development of a Spontaneous Liver Disease Resembling Autoimmune Hepatitis in Mice Lacking Tyro3, Axl and Mer Receptor Tyrosine Kinases
    Authors: Nan Qi, Peipei Liu, Yue Zhang, Hui Wu, Yongmei Chen, Daishu Han
    PLoS ONE
  2. Stromal changes in the aged lung induce an emergence from melanoma dormancy
    Authors: ME Fane, Y Chhabra, GM Alicea, DA Maranto, SM Douglass, MR Webster, VW Rebecca, GE Marino, F Almeida, BL Ecker, DJ Zabransky, L Hüser, T Beer, HY Tang, A Kossenkov, M Herlyn, DW Speicher, W Xu, X Xu, EM Jaffee, JA Aguirre-Gh, AT Weeraratna
    Nature, 2022-06-01;606(7913):396-405.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. Growth Arrest-Specific 6 Enhances the Suppressive Function of CD4(+)CD25(+) Regulatory T Cells Mainly through Axl Receptor
    Authors: GJ Zhao, JY Zheng, JL Bian, LW Chen, N Dong, Y Yu, GL Hong, A Chandoo, YM Yao, ZQ Lu
    Mediators Inflamm, 2017-02-08;2017(0):6848430.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: Neutralization, Western Blot
  4. Toll-like receptor-mediated inhibition of Gas6 and ProS expression facilitates inflammatory cytokine production in mouse macrophages.
    Authors: Deng T, Zhang Y, Chen Q, Yan K, Han D
    Immunology, 2012-01-01;135(1):40-50.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Toll-like receptor-initiated testicular innate immune responses in mouse Leydig cells.
    Authors: Shang T, Zhang X, Wang T, Sun B, Deng T, Han D
    Endocrinology, 2011-05-03;152(7):2827-36.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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