Mouse M-CSF R/CD115 Antibody Summary
Ala20-Ser511
Accession # P09581
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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M-CSF R/CD115 in Mouse Splenocytes. M-CSF R/CD115 was detected in immersion fixed mouse splenocytes using Mouse M-CSF R/CD115 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3818) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (orange; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: M-CSF R/CD115
M-CSF receptor, the product of the c-fms proto-oncogene, is a member of the type III subfamily of receptor tyrosine kinases that also includes receptors for SCF and PDGF. These receptors each contain five immunoglobulin-like domains in their extracellular domain (ECD) and a split kinase domain in their intracellular region (1-4). M-CSF receptor is expressed primarily on cells of the monocyte/macrophage lineage, dendritic cells, stem cells and in the developing placenta (1). Mouse M-CSF receptor cDNA encodes a 977 amino acid (aa) type I membrane protein with a 19 aa signal peptide, a 492 aa extracellular region containing the ligand-binding domain, a 25 aa transmembrane domain and a 441 aa cytoplasmic domain. The mouse M-CSF R ECD shares > 99% aa identity with rat and 60-63% aa identity with corresponding sequences in human, canine, feline and bovine M-CSF R. Activators of protein kinase C induce TACE/ADAM17 cleavage of the M-CSF receptor, releasing the functional ligand-binding extracellular domain (5). M-CSF binding induces receptor homodimerization, resulting in transphosphorylation of specific cytoplasmic tyrosine residues and signal transduction (6). The intracellular domain of activated M-CSF R binds more than 150 proteins that affect cell proliferation, survival, differentiation and cytoskeletal reorganization. Among these, PI3Kinase, P42/44 ERK and c-Cbl are key transducers of M-CSF R signals (3, 4). M-CSF R engagement is continuously required for macrophage survival and regulates lineage decisions and maturation of monocytes, macrophages, osteoclasts and DC (3, 4). M-CSF R and integrin alpha v beta 3 share signaling pathways during osteoclastogenesis, and deletion of either causes osteopetrosis (7, 8). In the brain, microglia expressing increased M-CSF R are concentrated with Alzheimers a beta peptide, but their role in pathogenesis is unclear (9, 10).
- deParseval, N. et al. (1993) Nucleic Acids Res. 21:750.
- Rothwell, V.M. and L.R. Rohrschneider (1987) Oncogene Res. 1:311.
- Chitu, V. and E.R. Stanley (2006) Curr. Opin. Immunol. 18:39.
- Ross, F.P. and S.L. Teitelbaum (2005) Immunol. Rev. 208:88.
- Rovida, E. et al. (2001) J. Immunol. 166:1583.
- Yeung, Y. et al. (1998) J. Biol. Chem. 273:17128.
- Dai, X. et al. (2002) Blood 99:111.
- Faccio, R. et al. (2003) J. Clin. Invest. 111:749.
- Li, M. et al. (2004) J. Neurochem. 91:623.
- Mitrasinovic, O.M. et al. (2005) J. Neurosci. 25:4442.
Product Datasheets
Citations for Mouse M-CSF R/CD115 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
11
Citations: Showing 1 - 10
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Single-Cell RNA Sequencing Reveals a Dynamic Stromal Niche That Supports Tumor Growth
Authors: S Davidson, M Efremova, A Riedel, B Mahata, J Pramanik, J Huuhtanen, G Kar, R Vento-Torm, T Hagai, X Chen, MA Haniffa, JD Shields, SA Teichmann
Cell Rep, 2020-05-19;31(7):107628.
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Minocycline protects against microgliopathy in a Csf1r haplo-insufficient mouse model of adult-onset leukoencephalopathy with axonal spheroids and pigmented glia (ALSP)
Authors: Li, X;Hu, B;Guan, X;Wang, Z;Zhou, Y;Sun, H;Zhang, X;Li, Y;Huang, X;Zhao, Y;Wang, X;Xu, H;Zhang, YW;Wang, Z;Zheng, H;
Journal of neuroinflammation
Species: Mouse
Sample Types:
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Ileitis-associated tertiary lymphoid organs originate at lymphatic valves and obstruct mesenteric lymph flow in response to tumor necrosis factor
Authors: Rafael S. Czepielewski, Emma C. Erlich, Emily J. Onufer, Shannon Young, Brian T. Saunders, Yong-Hyun Han et al.
Immunity
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RIPK1 activation mediates neuroinflammation and disease progression in multiple sclerosis
Authors: M Zelic, F Pontarelli, L Woodworth, C Zhu, A Mahan, Y Ren, M LaMorte, R Gruber, A Keane, P Loring, L Guo, TH Xia, B Zhang, P Orning, E Lien, A Degterev, T Hammond, D Ofengeim
Cell Reports, 2021-05-11;35(6):109112.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
RANKL/OPG ratio regulates odontoclastogenesis in damaged dental pulp
Authors: D Nishida, A Arai, L Zhao, M Yang, Y Nakamichi, K Horibe, A Hosoya, Y Kobayashi, N Udagawa, T Mizoguchi
Scientific Reports, 2021-02-25;11(1):4575.
Species: Mouse
Sample Types: Whole Cells, Whole Tissue
Applications: Flow Cytometry, IHC -
Conditional genetic deletion of CSF1 receptor in microglia ameliorates the physiopathology of Alzheimer's disease.
Authors: Pons V, Levesque P, Plante M, Rivest S
Alzheimers Res Ther, 2021-01-05;13(1):8.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Diverse Macrophage Populations Contribute to the Inflammatory Microenvironment in Premalignant Lesions During Localized Invasion
Authors: Ayman M. Ibrahim, Matthew A. Moss, Zane Gray, Michelle D. Rojo, Caitlin M. Burke, Kathryn L. Schwertfeger et al.
Frontiers in Oncology
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Peripheral nerve resident macrophages share tissue-specific programming and features of activated microglia
Authors: PL Wang, AKY Yim, KW Kim, D Avey, RS Czepielews, M Colonna, J Milbrandt, GJ Randolph
Nat Commun, 2020-05-21;11(1):2552.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Embryonic microglia influence developing hypothalamic glial populations
Authors: CM Marsters, D Nesan, R Far, N Klenin, QJ Pittman, DM Kurrasch
J Neuroinflammation, 2020-05-06;17(1):146.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Role of Macrophage Colony-Stimulating Factor Receptor on the Proliferation and Survival of Microglia Following Systemic Nerve and Cuprizone-Induced Injuries
Authors: Vincent Pons, Nataly Laflamme, Paul Préfontaine, Serge Rivest
Frontiers in Immunology
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Essential roles for the Tec family kinases Tec and Btk in M-CSF receptor signaling pathways that regulate macrophage survival.
Authors: Melcher M, Unger B, Schmidt U, Rajantie IA, Alitalo K, Ellmeier W
J. Immunol., 2008-06-15;180(12):8048-56.
Species: Mouse
Sample Types: Cell Lysates
Applications: Immunoprecipitation
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