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Mouse M-CSF R/CD115 Antibody

Catalog #: MAB3818
4 Citations Datasheet
Catalog # Availability Size / Price Qty
MAB3818
MAB3818-SP
Detection of M-CSF R/CD115 in RAW 264.7 Mouse Cell Line by Flow Cytometry.
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Citations (4)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse M-CSF R/CD115 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse M-CSF R/CD115 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human M-CSF R/CD115, recombinant mouse (rm) PDGF R beta, or rmFlt-3 Ligand is observed.
Source
Monoclonal Rat IgG2B Clone # 460615
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse M-CSF R/CD115
Ala20-Ser511
Accession # P09581
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of M-CSF R/CD115 antibody in RAW 264.7 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of M-CSF R/CD115 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse M-CSF R/CD115 Monoclonal Antibody (Catalog # MAB3818, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (Catalog # F0113).

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: M-CSF R/CD115

M-CSF receptor, the product of the c-fms proto-oncogene, is a member of the type III subfamily of receptor tyrosine kinases that also includes receptors for SCF and PDGF. These receptors each contain five immunoglobulin-like domains in their extracellular domain (ECD) and a split kinase domain in their intracellular region (1-4). M-CSF receptor is expressed primarily on cells of the monocyte/macrophage lineage, dendritic cells, stem cells and in the developing placenta (1). Mouse M-CSF receptor cDNA encodes a 977 amino acid (aa) type I membrane protein with a 19 aa signal peptide, a 492 aa extracellular region containing the ligand-binding domain, a 25 aa transmembrane domain and a 441 aa cytoplasmic domain. The mouse M-CSF R ECD shares >99% aa identity with rat and 60-63% aa identity with corresponding sequences in human, canine, feline and bovine M-CSF R. Activators of protein kinase C induce TACE/ADAM17 cleavage of the M-CSF receptor, releasing the functional ligand-binding extracellular domain (5). M-CSF binding induces receptor homodimerization, resulting in transphosphorylation of specific cytoplasmic tyrosine residues and signal transduction (6). The intracellular domain of activated M-CSF R binds more than 150 proteins that affect cell proliferation, survival, differentiation and cytoskeletal reorganization. Among these, PI3Kinase, P42/44 ERK and c-Cbl are key transducers of M-CSF R signals (3, 4). M-CSF R engagement is continuously required for macrophage survival and regulates lineage decisions and maturation of monocytes, macrophages, osteoclasts and DC (3, 4). M-CSF R and integrin alpha v beta 3 share signaling pathways during osteoclastogenesis, and deletion of either causes osteopetrosis (7, 8). In the brain, microglia expressing increased M-CSF R are concentrated with Alzheimers a beta peptide, but their role in pathogenesis is unclear (9, 10).

References
  1. deParseval, N. et al. (1993) Nucleic Acids Res. 21:750.
  2. Rothwell, V.M. and L.R. Rohrschneider (1987) Oncogene Res. 1:311.
  3. Chitu, V. and E.R. Stanley (2006) Curr. Opin. Immunol. 18:39.
  4. Ross, F.P. and S.L. Teitelbaum (2005) Immunol. Rev. 208:88.
  5. Rovida, E. et al. (2001) J. Immunol. 166:1583.
  6. Yeung, Y. et al. (1998) J. Biol. Chem. 273:17128.
  7. Dai, X. et al. (2002) Blood 99:111.
  8. Faccio, R. et al. (2003) J. Clin. Invest. 111:749.
  9. Li, M. et al. (2004) J. Neurochem. 91:623.
  10. Mitrasinovic, O.M. et al. (2005) J. Neurosci. 25:4442.
Long Name
Macrophage Colony Stimulating Factor Receptor
Entrez Gene IDs
1436 (Human); 12978 (Mouse)
Alternate Names
CD115 antigen; CD115; c-fms; colony stimulating factor 1 receptor; CSF1R; CSF-1-R; CSFR; EC 2.7.10.1; FMS proto-oncogene; FMSFIM2; macrophage colony stimulating factor I receptor; macrophage colony-stimulating factor 1 receptor; McDonough feline sarcoma viral (v-fms) oncogene homolog; M-CSF R; MCSFR; M-CSFR; Proto-oncogene c-Fms

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Citations for Mouse M-CSF R/CD115 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Distinct Populations of Immune-Suppressive Macrophages Differentiate from Monocytic Myeloid-Derived Suppressor Cells in Cancer
    Authors: T Kwak, F Wang, H Deng, T Condamine, V Kumar, M Perego, A Kossenkov, LJ Montaner, X Xu, W Xu, C Zheng, LM Schuchter, RK Amaravadi, TC Mitchell, GC Karakousis, C Mulligan, B Nam, G Masters, N Hockstein, J Bennett, Y Nefedova, DI Gabrilovic
    Cell Reports, 2020-12-29;33(13):108571.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. HLA-DR7 and HLA-DQ2: Transgenic mouse strains tested as a model system for ximelagatran hepatotoxicity
    Authors: H Lundgren, K Martinsson, K Cederbrant, J Jirholt, D Mucs, K Madeyski-B, S Havarinasa, P Hultman
    PLoS ONE, 2017-09-21;12(9):e0184744.
    Species: Mouse
    Sample Types: Plasma
    Applications: ELISA Development (Capture)
  3. Osteoprotection by semaphorin 3A.
    Authors: Hayashi M, Nakashima T, Taniguchi M, Kodama T, Kumanogoh A, Takayanagi H
    Nature, 2012-05-03;485(7396):69-74.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Generation of mature human myelomonocytic cells through expansion and differentiation of pluripotent stem cell-derived lin-CD34+CD43+CD45+ progenitors.
    Authors: Choi KD, Vodyanik MA, Slukvin II
    J. Clin. Invest., 2009-08-10;119(9):2818-29.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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