Mouse LAP (TGF-beta 1) Antibody

Catalog #: MAB7666
12 Citations 1 Review Datasheet

Catalog #	Availability	Size / Price	Qty
MAB7666
MAB7666-SP

Detection of LAP (TGF‑ beta 1) in Mouse iTreg cells by Flow Cytometry.

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All LAP (TGF-beta 1) Antibodies

Product Details

Citations (12)

FAQs

Supplemental Products

Reviews (1)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse LAP (TGF-beta 1) Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse LAP (TGF-beta 1) in flow cytometry.

Source

Monoclonal Rat IgG_2A Clone # 860206

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse LAP (TGF-beta 1)
Met1-Ser390
Accession # P04202

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of LAP (TGF‑ beta 1) in Mouse iTreg cells by Flow Cytometry. Mouse splenocytes treated with 10 µg/mL Anti-CD3, 5 µg/mL Anti-CD28, 10 µg/mL Recombinant Human TGF-beta 1 (Catalog # 240-B), and 20 µg/mL Recombinant Mouse IL-2 (Catalog # 402-IL) for 24 hours to induce T regulatory cells (iTregs) were stained with Rat Anti-Mouse CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB554P) and either (A) Rat Anti-Mouse LAP (TGF-beta 1) Monoclonal Antibody (Catalog # MAB7666) or (B) Rat IgG_2AIsotype Control (Catalog # MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LAP (TGF-beta 1)

TGF-beta 1 (Transforming Growth Factor beta 1) is one of three closely related mammalian members of the large TGF-beta superfamily that share a characteristic cystine knot structure (1‑7). TGF-beta 1, -2 and -3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition (1‑4). Each TGF-beta isoform has some non‑redundant functions; for TGF-beta 1, mice with targeted deletion show defects in hematopoiesis and endothelial differentiation, and die of overwhelming inflammation (2). Human TGF‑ beta 1 cDNA encodes a 390 amino acid (aa) precursor that contains a 29 aa signal peptide and a 361 aa proprotein (8). A furin‑like convertase processes the proprotein to generate an N‑terminal 249 aa Latency‑Associated Peptide (LAP) and a C‑terminal 112 aa mature TGF‑ beta 1 (8, 9). Disulfide‑linked homodimers of LAP and TGF‑ beta 1 remain non‑covalently associated after secretion, forming the small latent TGF‑ beta 1 complex (8‑10). Covalent linkage of LAP to one of three latent TGF‑ beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix (9, 10). TGF‑ beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins (10). Mature human TGF‑ beta 1 shares 100% aa identity with pig, dog and cow TGF‑ beta 1, and 99% aa identity with mouse, rat and horse TGF‑ beta 1. It demonstrates cross‑species activity (1). TGF‑ beta 1 signaling begins with high‑affinity binding to a type II ser/thr kinase receptor termed TGF‑ beta RII. This receptor then phosphorylates and activates a second ser/thr kinase receptor, TGF‑ beta RI, also known as Activin Receptor‑Like Kinase 5(ALK‑5), or alternatively, ALK‑1. This complex phosphorylates and activates Smad proteins that regulate transcription (3, 11, 12). Contributions of the accessory receptors Betaglycan (also known as TGF‑ beta RIII) and Endoglin, or use of Smad‑independent signaling pathways, allow for disparate actions observed in response to TGF‑ beta in different contexts (11).

References

Derynck, R. and K. Miyazono (2008) Cold Spring Harbor Laboratory Press, 29.
Dunker, N. and K. Krieglstein (2000) Eur. J. Biochem. 267:6982.
Wahl, S.M. (2006) Immunol. Rev. 213:213.
Chang, H. et al. (2002) Endocr. Rev. 23:787.
Lin, J.S. et al. (2006) Reproduction 132:179.
Hinck, A.P. et al. (1996) Biochemistry 35:8517.
Mittl, P.R.E. et al. (1996) Protein Sci. 5:1261.
Derynck, R. et al. (1985) Nature 316:701.
Miyazono, K. et al. (1988) J. Biol. Chem. 263:6407.
Oklu, R. and R. Hesketh (2000) Biochem. J. 352:601.
de Caestecker, M. et al. (2004) Cytokine Growth Factor Rev. 15:1.
Zuniga, J.E. et al. (2005) J. Mol. Biol. 354:1052.

Long Name

Latency-associated Peptide

Entrez Gene IDs

7040 (Human)

Alternate Names

CED; DPD1; LAP (TGFbeta 1); LAP (TGF-beta 1); LAP; TGFB; TGFB1; TGFbeta; transforming growth factor beta 1

Product Datasheets

Product Datasheet

Related Research Areas

Citations for Mouse LAP (TGF-beta 1) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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Inhibition of Xanthine Oxidase Protects against Diabetic Kidney Disease through the Amelioration of Oxidative Stress via VEGF/VEGFR Axis and NOX-FoxO3a-eNOS Signaling Pathway
Authors: KJ Yang, WJ Choi, YK Chang, CW Park, SY Kim, YA Hong
International Journal of Molecular Sciences, 2023-02-14;24(4):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Cyclo(Val-Pro) and Cyclo(Leu-Hydroxy-Pro) from Pseudomonas sp. (ABS-36) alleviates acute and chronic renal injury under in vitro and in vivo models (Ischemic reperfusion and unilateral ureter obstruction)
Authors: K Hira, P Sharma, A Mahale, O Prakash Ku, A Sajeli Beg
International immunopharmacology, 2021-12-29;103(0):108494.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Alcohol induces TGF&beta1 via downregulation of miR-1946a in murine lung fibroblast
Authors: X Fan, ST Mills, MJ Kaalla, V Sueblinvon
Sci Rep, 2020-11-05;10(1):19089.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization
L-Carnitine protects against tacrolimus-induced renal injury by attenuating programmed cell death via PI3K/AKT/PTEN signaling
Authors: HL Zheng, HY Zhang, CL Zhu, HY Li, S Cui, J Jin, SG Piao, YJ Jiang, MY Xuan, JZ Jin, YS Jin, JP Lee, BH Chung, BS Choi, CW Yang, C Li
Acta Pharmacol. Sin., 2020-06-17;0(0):.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC
Nanoparticle-based targeted delivery of pentagalloyl glucose reverses elastase-induced abdominal aortic aneurysm and restores aorta to the healthy state in mice
Authors: S Dhital, NR Vyavahare
PLoS ONE, 2020-03-27;15(3):e0227165.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Bone Marrow-Derived Mesenchymal Stem Cell Potential Regression of Dysplasia Associating Experimental Liver Fibrosis in Albino Rats
Authors: YH Khalifa, GM Mourad, WM Stephanos, SA Omar, RA Mehanna
Biomed Res Int, 2019-11-06;2019(0):5376165.
Species: Rat
Sample Types: Whole Tissue
Applications: Confocal Microscopy
AAV serotype 8-mediated liver specific GNMT expression delays progression of hepatocellular carcinoma and prevents carbon tetrachloride-induced liver damage
Authors: CC Fang, CF Wu, YJ Liao, SF Huang, M Chen, YA Chen
Sci Rep, 2018-09-14;8(1):13802.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Enteroendocrine cells switch hormone expression along the crypt-to-villus BMP signalling gradient
Authors: J Beumer, B Artegiani, Y Post, F Reimann, F Gribble, TN Nguyen, H Zeng, M Van den Bo, JH Van Es, H Clevers
Nat. Cell Biol., 2018-07-23;20(8):909-916.
Species: Mouse
Sample Types: Organoids
Applications: Bioassay
Suppressive IL-17A(+)Foxp3(+) and ex-Th17 IL-17A(neg)Foxp3(+) Treg cells are a source of tumour-associated Treg cells
Authors: S Downs-Cann, S Berkey, GM Delgoffe, RP Edwards, T Curiel, K Odunsi, DL Bartlett, N Obermajer
Nat Commun, 2017-03-14;8(0):14649.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization
Culture supernatant of adipose stem cells can ameliorate allergic airway inflammation via recruitment of CD4(+)CD25(+)Foxp3 T cells
Authors: HS Yu, MK Park, SA Kang, KS Cho, SJ Mun, HJ Roh
Stem Cell Res Ther, 2017-01-23;8(1):8.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: Western Blot

Extracellular Adenosine Production by ecto-5'-Nucleotidase (CD73) Enhances Radiation-Induced Lung Fibrosis
Authors: F Wirsdörfer, S de Leve, F Cappuccini, T Eldh, AV Meyer, E Gau, LF Thompson, NY Chen, H Karmouty-Q, U Fischer, M Kasper, D Klein, JW Ritchey, MR Blackburn, AM Westendorf, M Stuschke, V Jendrossek
Cancer Res., 2016-02-26;76(10):3045-56.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

RhoA/phosphatidylinositol 3-kinase/protein kinase B/mitogen-activated protein kinase signaling after growth arrest-specific protein 6/mer receptor tyrosine kinase engagement promotes epithelial cell growth and wound repair via upregulation of hepatocyte growth factor in macrophages.
Authors: Lee Y, Park H, Woo S, Park E, Kang J
J Pharmacol Exp Ther, 2014-06-17;350(3):563-77.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization