Mouse Kallikrein 5 Antibody Summary
23-293
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Mouse Kallikrein 5 by Western Blot. Western blot shows lysates of NMuMG mouse mammary gland epithelial cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse Kallikrein 5 Monoclonal Antibody (Catalog # MAB7236) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Kallikrein 5 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Kallikrein 5 in Hair Follicle of Mouse Skin. Kallikrein 5 was detected in immersion fixed frozen sections of mouse skin using Rat Anti-Mouse Kallikrein 5 Monoclonal Antibody (Catalog # MAB7236) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to the inner sheath cuticle of hair follicles. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Kallikrein 5
Tissue Kallikrein 5 (KLK5) is a secreted serine protease with trypsin‑like substrate specificity. KLK5 is highly expressed in the skin, and is also known as stratum corneum tryptic enzyme due to its high expression level in this tissue. KLK5 is thought to play an important role in the process of desquamation (1). KLK5 is a potential biomarker for ovarian and breast cancers (2). It may also be a physiological activator of KLK7 in the stratum corneum (3). Recombinant mouse KLK5 is purified as the proform, and can undergo autoactivation under the appropriate conditions. KLK5 enzymatic activity can be inhibited by the following recombinant human serpins: Serpin‑A1, ‑A5, ‑F2, and ‑G1 (Catalog # 1268-PI, 1266-PI, 1470-PI, and 2488-PI).
- Brattsand M. and T. Egelrud (1999) J. Biol. Chem. 274:30033.
- Yousef G.M. et al. (2003) Cancer Res. 63:3958.
- Kim H. et al. (2001) Br. J. Cancer 84:543.
Product Datasheets
Citations for Mouse Kallikrein 5 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Kallikrein 5 Inhibition by the Lympho-Epithelial Kazal-Type Related Inhibitor Hinders Matriptase-Dependent Carcinogenesis
Authors: Elaine Zayas Marcelino da Silva, Thais Fernanda de Campos Fraga-Silva, Yao Yuan, Márcia Gaião Alves, Gabriel Azevedo Publio, Carol Kobori da Fonseca et al.
Cancers (Basel)
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Keratinocytes coordinate inflammatory responses and regulate development of secondary lymphedema
Authors: HJ Park, RP Kataru, J Shin, GD Garc A Nor, EM Encarnacio, MG Klang, E Riedel, M Coriddi, JH Dayan, BJ Mehrara
bioRxiv : the preprint server for biology, 2023-01-21;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
VPS33B and VIPAR are essential for epidermal lamellar body biogenesis and function.
Authors: Rogerson C, Gissen P.
Biochim. Biophys. Acta.
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