Mouse Integrin beta 6 Antibody

Catalog #: MAB2389 Datasheet

Catalog #	Availability	Size / Price	Qty
MAB2389
MAB2389-SP

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Mouse Integrin beta 6 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Integrin beta 6 in direct ELISAs and Western blots.

Source

Monoclonal Rat IgG_2A Clone # 268622

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin beta 6
Gly22-Asn706
Accession # Q9Z0T9

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse Integrin beta 6 under non-reducing conditions only

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Integrin beta 6 in XB2 Mouse Cell Line. Integrin beta 6 was detected in immersion fixed XB2 mouse teratoma keratinocyte cell line using Rat Anti-Mouse Integrin beta 6 Monoclonal Antibody (Catalog # MAB2389) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (yellow; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin beta 6

Integrin beta 6 is a type I transmembrane Integrin beta subunit that heterodimerizes with alpha V subunits in the epithelial restricted integrin alpha V/ beta 6. The extracellular domain of mouse Integrin beta 6 contains a collagen binding von-Willebrand factor A domain and multiple truncated EGF-like motifs. Mouse Integrin beta 6 extracellular domain shares 96% and 91% amino acid sequence identity with the corresponding regions of the rat and human protein, respectively.

Entrez Gene IDs

3694 (Human); 16420 (Mouse); 311061 (Rat); 102122296 (Cynomolgus Monkey)

Alternate Names

Integrin beta 6; integrin beta-6; integrin, beta 6; ITGB6

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