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Mouse IL-7 Antibody

Catalog #: AB-407-NA
4 Citations Datasheet
Catalog # Availability Size / Price Qty
AB-407-NA
Cell Proliferation Induced by IL‑7 and Neutralization by Mouse IL‑7 Antibody.
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Citations (4)
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Mouse IL-7 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-7 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human IL-7 is observed.
Source
Polyclonal Goat IgG
Purification
Protein A or G purified
Immunogen
E. coli-derived recombinant mouse IL-7 (R&D Systems, Catalog # 407-ML)
Glu26-Ile154
Accession # Q544C8
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse IL‑7 (Catalog # 407-ML)
Neutralization
Measured by its ability to neutralize IL‑7-induced proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC). Yokota, T. et al. (1986) Proc. Natl. Acad. Sci. USA 83:5894. The Neutralization Dose (ND50) is typically 0.5-1.5 µg/mL in the presence of 1.5 ng/mL Recombinant Mouse IL‑7.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by IL‑7 and Neutralization by Mouse IL‑7 Antibody. View Larger

Cell Proliferation Induced by IL‑7 and Neutralization by Mouse IL‑7 Antibody. Recombinant Mouse IL‑7 (Catalog # 407-ML) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑7 (1.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL‑7 Polyclonal Antibody (Catalog # AB-407-NA). The ND50 is typically 0.5-1.5 µg/mL.

Preparation and Storage

Reconstitution
Reconstitute at 1 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-7

IL-7, previously known as pre-B cell growth factor and lymphopoietin-1, was originally purified on the basis of its ability to promote the proliferation of precursor B cells. It has now been shown that IL-7 can also stimulate the proliferation of thymocytes, T cell progenitors and mature CD4 + and CD8+ T cells. IL-7 can induce the formation of lymphokine-activated killer (LAK) cells as well as the development of cytotoxic T lymphocytes (CTL). IL-7 was also shown to induce the V(D)J rearrangement of the T cell receptor beta gene in mouse fetal thymocytes. Among myeloid lineage cells, IL-7 can up-regulate the production of pro-inflammatory cytokines and stimulate the tumorocidal activity of monocytes/macrophages. IL-7 is expressed by adherent stromal cells from various tissues.

Mouse IL-7 cDNA encodes a precursor protein of 154 amino residues containing a 25 amino acid residue signal peptide. Human IL-7 has approximately 65% amino acid sequence identity with mouse IL-7 and both proteins exhibit cross-species activity.

IL-7 bioactivities are mediated by the binding of IL-7 to functional high-affinity receptor complexes. The ligand binding subunit (IL-7 R) of the IL-7 receptor complex has been cloned from human and mouse sources. In addition to the membrane-anchored form of the IL-7 receptor, a human cDNA clone that encodes a soluble form of the IL-7 R has also been isolated. The gamma  chain of the IL-2 receptor complex has been shown to be an essential component for IL-7 signal transduction. Both IL-7 R and IL-2 R gamma are members of the hematopoietin receptor superfamily. Cells known to express IL-7 receptors include pre-B cells, T cells, and bone marrow cells.

Long Name
Interleukin 7
Entrez Gene IDs
3574 (Human); 16196 (Mouse); 25647 (Rat)
Alternate Names
IL7; IL-7; IL-7interleukin-7; interleukin 7; Lymphopoietin-1; PBGF

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Citations for Mouse IL-7 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Multicolor quantitative confocal imaging cytometry
    Authors: DL Coutu, KD Kokkaliari, L Kunz, T Schroeder
    Nat. Methods, 2017-11-13;15(1):39-46.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Interleukin-7 and -15 maintain pathogenic memory Th17 cells in autoimmunity
    Authors: Yihe Chen
    J. Autoimmun, 2016-11-26;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  3. IL-7 Promotes TH1 Cell Differentiation and Its Level in Serum Predicts Response to Interferon-beta in Multiple Sclerosis
    Authors: Li-Fen Lee, Robert Axtell, Guang Huan Tu, Kathryn Logronio, Jeanette Dilley, Jessica Yu et al.
    Science Translational Medicine
  4. Crucial role of interleukin-7 in T helper type 17 survival and expansion in autoimmune disease.
    Authors: Liu X, Leung S, Wang C, Tan Z, Wang J, Guo TB, Fang L, Zhao Y, Wan B, Qin X, Lu L, Li R, Pan H, Song M, Liu A, Hong J, Lu H, Zhang JZ
    Nat. Med., 2010-01-10;16(2):191-7.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization

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