Mouse IL-4 ELISpot Kit

Catalog #: EL404
10 Citations Datasheet

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Citations (10)

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Summary Product Datasheets Preparation and Storage Background

Mouse IL-4 ELISpot Kit Summary

Assay Type

Quantitative Sandwich ELISA

Format

96-well PVDF-backed microplate

Assay Length

3 hours 15 mins to 4 hours 30 mins*

Sample Type

Whole Cells

Specificity

Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

PRODUCT SUMMARY
ELISpot kits are highly sensitive, microplate-based assays for the detection of cytokine secreting cells. This kit is designed for the detection and enumeration of mouse IL-4. Complete ELISpot kits are ready-to-run and require no assay development or refinement.

This ELISpot assay employs a capture antibody specific for mouse IL-4, pre-coated onto a PVDF-backed microplate. Appropriately stimulated cells are pipetted directly into the wells and the immobilized antibody in the immediate vicinity of the secreting cells binds secreted mouse IL-4. Following wash steps and incubation with a biotinylated detection antibody, alkaline-phosphatase conjugated to streptavidin is added. Unbound enzyme is subsequently removed by washing and a substrate solution (BCIP/NBT) is added. A blue-black colored precipitate forms at the sites of cytokine localization and appears as spots, with each individual spot representing an individual mouse IL-4 secreting cell. The spots can be counted with an automated ELISpot reader system or manually using a stereomicroscope.

PRODUCT FEATURES

Detect and quantitate individual cells secreting mouse IL-4
High sensitivity - ELISpot assays can measure responses with frequencies well below 1 in 100,000 cells
No in vitro expansion of cells required
High-throughput - ELISpot assays use only a small number of primary cells

KIT CONTENTS

Mouse IL-4 Microplate
Biotinylated Detection Antibody
Streptavidin conjugated to Alkaline Phosphatase
Dilution Buffers
Wash Buffer Concentrate
BCIP/NBT Chromogen
Mouse IL-4 Positive Control

OTHER REAGENTS REQUIRED

Pipettes and pipette tips
Deionized or distilled water
Squirt bottle, manifold dispenser, or automated microplate washer
500 mL graduated cylinder
37 °C CO₂ incubator
Sterile culture media
Dissection microscope or an automated ELISpot reader

Product Datasheets

Product Datasheet

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-4

IL-4 (Interleukin-4) is a cytokine that is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils. It promotes cell proliferation, survival, and immunoglobulin class switch to IgG4 and IgE in human B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells. IL-4 plays a dominant role in the development of allergic inflammation and asthma. The type I receptor for IL-4, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R? and the common ? chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on nonhematopoietic cells consists of IL-4 R? and IL-13 R?1. The type II receptor also transduces IL-13 mediated signals.

Long Name:

Interleukin 4

Entrez Gene IDs:

3565 (Human); 16189 (Mouse); 287287 (Rat); 397225 (Porcine); 280824 (Bovine); 403785 (Canine); 574281 (Primate); 100302454 (Rabbit)

Alternate Names:

B cell growth factor 1; BCDF; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; IL4; IL-4; IL-4B_cell stimulatory factor 1; IL4E12; interleukin 4; interleukin-4; Lymphocyte stimulatory factor 1; MGC79402; pitrakinra

⚠ WARNING: This product can expose you to chemicals including methanol, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov.

Citations for Mouse IL-4 ELISpot Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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A two-adjuvant multiantigen candidate vaccine induces superior protective immune responses against SARS-CoV-2 challenge
Authors: W Jiang, L Shi, L Cai, X Wang, J Li, H Li, J Liang, Q Gu, G Ji, J Li, L Liu, M Sun
Cell Reports, 2021-11-24;37(11):110112.
Species: Mouse
Sample Types: Whole Cells
Elongated TCR alpha chain CDR3 favors an altered CD4 cytokine profile.
Authors: Reynolds C, Chong D, Raynsford E, Quigley K, Kelly D, Llewellyn-Hughes J, Altmann D, Boyton R
BMC Biol, 2014-05-09;12(0):32.
Species: Mouse
Sample Types: Cell Culture Supernates
HIV-1 adenoviral vector vaccines expressing multi-trimeric BAFF and 4-1BBL enhance T cell mediated anti-viral immunity.
Authors: Kanagavelu, Saravana, Termini, James M, Gupta, Sachin, Raffa, Francesc, Fuller, Katherin, Rivas, Yaelis, Philip, Sakhi, Kornbluth, Richard, Stone, Geoffrey
PLoS ONE, 2014-02-28;9(2):e90100.
Species: Mouse
Sample Types: Whole Cells
Production and immunogenicity of chimeric virus-like particles containing the spike glycoprotein of infectious bronchitis virus.
Authors: Lv L, Li X, Liu G, Li R, Liu Q, Shen H, Wang W, Xue C, Cao Y
J Vet Sci, 2013-12-27;15(2):209-16.
Species: Mouse
Sample Types: Whole Cells
The Shc family protein adaptor, Rai, acts as a negative regulator of Th17 and Th1 cell development.
Authors: Savino, Maria Te, Ulivieri, Cristina, Emmi, Giacomo, Prisco, Domenico, De Falco, Giulia, Ortensi, Barbara, Beccastrini, Enrico, Emmi, Lorenzo, Pelicci, Giuliana, D'Elios, Mario M, Baldari, Cosima T
J Leukoc Biol, 2013-01-23;93(4):549-59.
Species: Mouse
Sample Types: Whole Cells
A VLP vaccine induces broad-spectrum cross-protective antibody immunity against H5N1 and H1N1 subtypes of influenza A virus.
Authors: Wu, Chia-Yin, Yeh, Yi-Chun, Chan, Jia-Tsro, Yang, Yu-Chih, Yang, Ji-Rong, Liu, Ming-Tsa, Wu, Ho-Sheng, Hsiao, Pei-Wen
PLoS ONE, 2012-08-07;7(8):e42363.
Species: Mouse
Sample Types: Whole Cells
TH17 cells mediate steroid-resistant airway inflammation and airway hyperresponsiveness in mice.
Authors: McKinley L, Alcorn JF, Peterson A, Dupont RB, Kapadia S, Logar A, Henry A, Irvin CG, Piganelli JD, Ray A, Kolls JK
J. Immunol., 2008-09-15;181(6):4089-97.
Species: Mouse
Sample Types: Whole Cells
Critical role of donor tissue expression of programmed death ligand-1 in regulating cardiac allograft rejection and vasculopathy.
Authors: Yang J, Popoola J, Khandwala S, Vadivel N, Vanguri V, Yuan X, Dada S, Guleria I, Tian C, Ansari MJ, Shin T, Yagita H, Azuma M, Sayegh MH, Chandraker A
Circulation, 2008-01-22;117(5):660-9.
Species: Mouse
Sample Types: Whole Cells
Nitric oxide mediates T cell cytokine production and signal transduction in histidine decarboxylase knockout mice.
Authors: Koncz A, Pasztoi M, Mazan M, Fazakas F, Buzas E, Falus A, Nagy G
J. Immunol., 2007-11-15;179(10):6613-9.
Species: Mouse
Sample Types: Whole Cells
Immunopharmacological and antitumor effects of second-generation immunomodulatory oligonucleotides containing synthetic CpR motifs.
Authors: Wang D, Li Y, Yu D, Song SS, Kandimalla ER, Agrawal S
Int. J. Oncol., 2004-04-01;24(4):901-8.
Species: Mouse
Sample Types: Whole Cells