Mouse IL-4 ELISpot Development Module, 5 Plate Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
PRODUCT SUMMARY
Complete ELISpot kits are highly sensitive, microplate-based assays for the detection of cytokine secreting cells. Kits are available for detection and enumeration of a single analyte or two analytes simultaneously. Complete ELISpot kits are ready-to-run and require no assay development or refinement. ELISpot Development Modules contain the basic components required to develop an ELISpot assay. They offer an economical alternative to buying separate antibodies.
ELISpot development modules are an alternative to ELISpot kits. A basic understanding of ELISpot assay development is required for the successful use of these reagents. Each investigator should optimize the coating conditions, the assay sensitivity, the type of enzyme and substrate, as well as the concentrations of the capture and detection antibodies to achieve desired results. The analyte-specific ELISpot Development Module and the ELISpot Blue Color Module contain the necessary components for analyte detection and visualization, respectively. These modules can be used together but are sold separately. Each module contains enough reagents for at least five 96-well microplates.
PRODUCT FEATURES
- An economical alternative to ELISpot Kits
- Optimized capture and detection antibody pairings and recommended concentrations save lengthy development time
- Generic development protocols provide direction to start an optimization protocol
- Customize the assay to your specific needs
MODULE CONTENTS
- Mouse IL-4 Capture Antibody
- Mouse IL-4 Biotinylated-conjugated Detection Antibody
OTHER REAGENTS REQUIRED
- ELISpot Blue Color Module or equivalent (R&D Systems, Catalog # SEL002)
- PBS - 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
- Wash Buffer - 0.05% Tween® 20 in PBS
- Blocking Buffer - 1% BSA, 5% Sucrose in PBS
- Reagent Diluent - 1% BSA in PBS, pH 7.2 - 7.4, 0.2 µm filtered
- 2 °C – 8 °C refrigerator
- 37 °C CO2 incubator
- Positive Control - Use Recombinant Mouse IL-4 or cells known to secrete Mouse IL-4
- 96-well plates - Nitrocellulose-bottom plates, PVDF-bottom Immunospot® plates, or flat-bottom polystyrene Immulon® ELISA plates
- Multi-channel pipette, squirt bottle, manifold dispenser, or automated microplate washer
- Dissection microscope or an automated ELISpot Reader
- Deionized H2O
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Product Datasheets
Preparation and Storage
Background: IL-4
IL-4 (Interleukin-4) is a cytokine that is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils. It promotes cell proliferation, survival, and immunoglobulin class switch to IgG4 and IgE in human B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells. IL-4 plays a dominant role in the development of allergic inflammation and asthma. The type I receptor for IL-4, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R? and the common ? chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on nonhematopoietic cells consists of IL-4 R? and IL-13 R?1. The type II receptor also transduces IL-13 mediated signals.
Citations for Mouse IL-4 ELISpot Development Module, 5 Plate
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 8
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Orientation of Antigen Display on Self-Assembling Protein Nanoparticles Influences Immunogenicity
Authors: CG Schneider, JA Taylor, MQ Sibilo, K Miura, KL Mallory, C Mann, C Karch, Z Beck, GR Matyas, CA Long, E Bergmann-L, P Burkhard, E Angov
Vaccines, 2021-01-29;9(2):.
Species: Mouse
Sample Types: Whole Cells
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Critical role for IL-18 in spontaneous lung inflammation caused by autophagy deficiency.
Authors: Abdel Fattah E, Bhattacharya A, Herron A, Safdar Z, Eissa N
J Immunol, 2015-04-17;194(11):5407-16.
Species: Mouse
Sample Types: Whole Cells
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Nanoparticle vaccines encompassing the respiratory syncytial virus (RSV) G protein CX3C chemokine motif induce robust immunity protecting from challenge and disease.
Authors: Jorquera P, Choi Y, Oakley K, Powell T, Boyd J, Palath N, Haynes L, Anderson L, Tripp R
PLoS ONE, 2013-09-10;8(9):e74905.
Species: Mouse
Sample Types: Whole Cells
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Inhibition of neointima formation through DNA vaccination for apolipoprotein(a): a new therapeutic strategy for lipoprotein(a).
Authors: Kyutoku, Mariko, Nakagami, Hironori, Koriyama, Hiroshi, Nakagami, Futoshi, Shimamura, Munehisa, Kurinami, Hitomi, Tomioka, Hideki, Miyake, Takashi, Katsuya, Tomohiro, Morishita, Ryuichi
Sci Rep, 2013-01-01;3(0):1600.
Species: Mouse
Sample Types: Whole Cells
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Estriol generates tolerogenic dendritic cells in vivo that protect against autoimmunity.
Authors: Papenfuss TL, Powell ND, McClain MA, Bedarf A, Singh A, Gienapp IE, Shawler T, Whitacre CC
J. Immunol., 2011-02-11;186(6):3346-55.
Species: Mouse
Sample Types: Whole Cells
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Mucosal delivery of a transmission-blocking DNA vaccine encoding Giardia lamblia CWP2 by Salmonella typhimurium bactofection vehicle.
Authors: Abdul-Wahid A, Faubert G
Vaccine, 2007-10-24;25(50):8372-83.
Species: Mouse
Sample Types: Whole Cells
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Amelioration of graft versus host disease by galectin-1.
Authors: Baum LG, Blackall DP, Arias-Magallano S, Nanigian D, Uh SY, Browne JM, Hoffmann D, Emmanouilides CE, Territo MC, Baldwin GC
Clin. Immunol., 2003-12-01;109(3):295-307.
Species: Mouse
Sample Types: Whole Cells
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Absence of the complement anaphylatoxin C3a receptor suppresses Th2 effector functions in a murine model of pulmonary allergy.
Authors: Drouin SM, Corry DB, Hollman TJ, Kildsgaard J, Wetsel RA
J. Immunol., 2002-11-15;169(10):5926-33.
Species: Mouse
Sample Types: Whole Cells
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