Mouse IL-4 Antibody

Catalog #: AF-404-NA
6 Citations 1 Review Datasheet

Catalog #	Availability	Size / Price	Qty
AF-404-NA
AF-404-SP

Cell Proliferation Induced by IL‑4 and Neutralization by Mouse IL‑4 Antibody.

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All IL-4 Antibodies

Product Details

Citations (6)

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Supplemental Products

Reviews (1)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IL-4 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IL-4 in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse IL-4
His23-Ser140
Accession # P07750

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse IL‑4 (Catalog # 404-ML)

Neutralization

Measured by its ability to neutralize IL‑4-induced proliferation in the HT‑2 mouse T cell line. The Neutralization Dose (ND₅₀) is typically 0.1-0.3 µg/mL in the presence of 7.5 ng/mL Recombinant Mouse IL‑4.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Cell Proliferation Induced by IL‑4 and Neutralization by Mouse IL‑4 Antibody. Recombinant Mouse IL‑4 (404-ML) stimulates proliferation in the HT‑2 mouse T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑4 (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-404-NA). The ND₅₀ is typically 0.1-0.3 µg/mL.

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-4

Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13-18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1‑4). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (5). Mouse IL-4 is synthesized with a 24 amino acid (aa) signal sequence. Mature mouse IL-4 shares 39%, 39%, and 59% aa sequence identity with bovine, human, and rat IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities (6-8). IL-4 exerts its effects through two receptor complexes (9, 10). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4⁺ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgG1 and IgE in mouse B cells, acquisition of the Th2 phenotype by naïve CD4⁺ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (11-14). IL-4 plays a dominant role in the development of allergic inflammation and asthma (13, 15).

References

Benczik, M. and S.L. Gaffen (2004) Immunol. Invest. 33:109.
Chomarat, P. and J. Banchereau (1998) Int. Rev. Immunol. 17:1.
Lee, F. et al. (1986) Proc. Natl. Acad. Sci. USA 83:2061.
Noma, Y. et al. (1986) Nature 319:640.
Redfield, C. et al. (1991) Biochemistry 30:11029.
Ramirez, F. et al. (1988) J. Immunol. Meth. 221:141.
Leitenberg, D. and T.L. Feldbush (1988) Cell. Immunol. 111:451.
Mosman, T.R. et al. (1987) J. Immunol. 138:1813.
Mueller, T.D. et al. (2002) Biochim. Biophys. Acta 1592:237.
Nelms, K. et al. (1999) Annu. Rev. Immunol. 17:701.
Paludan, S.R. (1998) Scand. J. Immunol. 48:459.
Corthay, A. (2006) Scand. J. Immunol. 64:93.
Ryan, J.J. et al. (2007) Crit. Rev. Immunol. 27:15.
Grone, A. (2002) Vet. Immunol. Immunopathol. 88:1.
Rosenberg, H.F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.

Long Name

Interleukin 4

Entrez Gene IDs

3565 (Human); 16189 (Mouse); 287287 (Rat); 397225 (Porcine); 280824 (Bovine); 403785 (Canine); 574281 (Primate); 100302454 (Rabbit)

Alternate Names

B cell growth factor 1; BCDF; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; IL4; IL-4; IL-4B_cell stimulatory factor 1; IL4E12; interleukin 4; interleukin-4; Lymphocyte stimulatory factor 1; MGC79402; pitrakinra

Product Datasheets

Product Datasheet

Related Research Areas

Citations for Mouse IL-4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Mechanical stimulation improves rotator cuff tendon-bone healing via activating IL-4/JAK/STAT signaling pathway mediated macrophage M2 polarization
Authors: Y Liu, L Wang, S Li, T Zhang, C Chen, J Hu, D Sun, H Lu
Journal of Orthopaedic Translation, 2022-10-06;37(0):78-88.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo
Dual vaccination against IL-4 and IL-13 protects against chronic allergic asthma in mice
Authors: E Conde, R Bertrand, B Balbino, J Bonnefoy, J Stackowicz, N Caillot, F Colaone, S Hamdi, R Houmadi, A Loste, JBJ Kamphuis, F Huetz, L Guillemina, N Gaudenzio, A Mougel, D Hardy, JN Snouwaert, BH Koller, V Serra, P Bruhns, G Grouard-Vo, LL Reber
Nature Communications, 2021-05-11;12(1):2574.
Species: Mouse
Sample Types: Recombinant Proteins
Applications: Western Blot
Murine IL-4?2 splice variant down-regulates IL-4 activities independently of IL-4R? binding and STAT-6 phosphorylation
Authors: GR Diogo, A Sparrow, MJ Paul, A Copland, PJ Hart, S Stelter, C van Dollew, PMW Drake, DC Macallan, R Reljic
Cytokine, 2017-09-13;99(0):154-162.
Species: Mouse
Sample Types: Recombinant Protein
Applications: Western Blot
The IL-4/IL-13/Stat6 signalling pathway promotes luminal mammary epithelial cell development.
Authors: Khaled WT, Read EK, Nicholson SE, Baxter FO, Brennan AJ, Came PJ, Sprigg N, McKenzie AN, Watson CJ
Development, 2007-07-04;134(15):2739-50.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC
Optimizing plasmid-based gene transfer for investigating skeletal muscle structure and function.
Authors: Schertzer JD, Plant DR, Lynch GS
Mol. Ther., 2005-11-23;13(4):795-803.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
The IL-6 family cytokines, interleukin-6, interleukin-11, oncostatin M, and leukemia inhibitory factor, enhance mast cell growth through fibroblast-dependent pathway in mice.
Authors: Gyotoku E, Morita E, Kameyoshi Y, Hiragun T, Yamamoto S, Hide M
Arch. Dermatol. Res., 2001-11-01;293(10):508-14.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization