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Mouse IL-3 Antibody

Catalog #: AF-403-NA
3 Citations Datasheet
Catalog # Availability Size / Price Qty
AF-403-NA
AF-403-SP
Cell Proliferation Induced by IL‑3 and Neutralization by Mouse IL‑3 Antibody.
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Citations (3)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IL-3 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-3 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human IL-3 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse IL-3
Asp33-Cys166
Accession # P01586
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse IL‑3 (Catalog # 403-ML)
Neutralization
Measured by its ability to neutralize IL‑3-induced proliferation in the M‑NFS‑60 mouse myelogenous leukemia lymphoblast cell line. Holmes, K.L. et al. (1985) Proc. Natl. Acad. Sci. USA 82:6687. The Neutralization Dose (ND50) is typically 0.01-0.03 µg/mL in the presence of 0.5 ng/mL Recombinant Mouse IL‑3.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by IL‑3 and Neutralization by Mouse IL‑3 Antibody. View Larger

Cell Proliferation Induced by IL‑3 and Neutralization by Mouse IL‑3 Antibody. Recombinant Mouse IL‑3 (Catalog # 403-ML) stimulates proliferation in the M‑NFS‑60 mouse myelogenous leukemia lymphoblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑3 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-403-NA). The ND50 is typically 0.01-0.03 µg/mL.

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-3

Interleukin 3 is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. In addition, IL-3 also affects the functional activity of mature mast cells, basophils, eosinophils and macrophages. Because of its multiple functions and targets, it was originally studied under different names, including mast cell growth factor P-cell stimulating factor, burst promoting activity, multi-colony stimulating factor, thy-1 inducing factor and WEHI-3 growth factor. In addition to activated T cells, other cell types such as human thymic epithelial cells, activated mouse mast cells, mouse keratinocytes and neurons/astrocytes can also produce IL-3. At the amino acid sequence level, mature human and mouse IL-3 share only 29% sequence identity. Consistent with this lack of homology, IL-3 activity is highly species-specific and human IL-3 does not show activity on mouse cells.

IL-3 exerts its biological activities through binding to specific cell surface receptors. The high affinity receptor responsible for IL-3 signaling is composed of alpha and beta subunits. The IL-3R alpha is a member of the cytokine receptor super family and binds IL-3 with low affinity. Two distinct beta subunits, AIC2A ( beta IL-3) and AIC2B ( beta c) are present in mouse cells. beta IL-3 also binds IL-3 with low affinity and forms a high affinity receptor with the alpha subunit. The beta c subunits does not bind any cytokine but forms functional high affinity receptors with the alpha subunit of the IL-3, IL-5 and GM-CSF receptors. Receptors for IL-3 are present on bone marrow progenitors, macrophages, mast cells, eosinophils, megakaryocytes, basophils, and various myeloid leukemic cells.

References
  1. Yokota, T. et al. (1984) Proc. Natl. Acad. Sci. USA 81:1070.
  2. Fung, M.C. et al. (1984) Nature 307:233.
  3. Miyatake, S. et al. (1985) Proc. Natl. Acad. Sci. USA 82:316.
Long Name
Interleukin 3
Entrez Gene IDs
3562 (Human); 16187 (Mouse); 24495 (Rat)
Alternate Names
Hematopoietic growth factor; IL3; IL-3; IL-3MGC79398; interleukin 3 (colony-stimulating factor, multiple); interleukin-3; Mast cell growth factor; mast-cell growth factor; MCGF; MCGFMGC79399; MULTI-CSF; multilineage-colony-stimulating factor; Multipotential colony-stimulating factor; P-cell stimulating factor; P-cell-stimulating factor

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Citations for Mouse IL-3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Interleukin-3 plays a vital role in hyperoxic acute lung injury in mice via mediating inflammation
    Authors: Z Huang, W Zhang, J Yang, F Sun, H Zhou
    BMC Pulm Med, 2018-10-30;18(1):164.
    Species: Mouse
    Sample Types: Plasma
    Applications: Western Blot
  2. Neuritogenic effects of T cell-derived IL-3 on mouse splenic sympathetic neurons in vivo.
    Authors: Kannan-Hayashi Y, Okamura K, Hattori S, Kuwamura M, Higuchi E, Terayama H, Moriyama M, Mukamoto M, Okada M, Ohsugi Y, Nakamura Y
    J. Immunol., 2008-03-15;180(6):4227-34.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  3. A secreted and LIF-mediated stromal cell-derived activity that promotes ex vivo expansion of human hematopoietic stem cells.
    Authors: Shih CC, Hu MC, Hu J
    Blood, 2000-03-15;95(6):1957-66.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization

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