Mouse IL-3 Antibody

Catalog #: AB-403-NA Datasheet

Catalog #	Availability	Size / Price	Qty
AB-403-NA

Cell Proliferation Induced by IL‑3 and Neutralization by Mouse IL‑3 Antibody.

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Mouse IL-3 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IL‑3 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant rat IL-3 is observed and less than 1% cross-reactivity with recombinant human IL-3 is observed. Neutralizes the biological activity of recombinant mouse IL-3, but will not neutralize the biological activity of recombinant human IL-3.

Source

Polyclonal Goat IgG

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant mouse IL-3
Asp33-Cys166
Accession # P01586

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse IL‑3 (Catalog # 403-ML)

Neutralization

Measured by its ability to neutralize IL‑3-induced proliferation in the NFS60 mouse myeloid cells. The Neutralization Dose (ND₅₀) is typically 0.015-0.025 µg/mL in the presence of 0.5 ng/mL Recombinant Mouse IL‑3.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Cell Proliferation Induced by IL‑3 and Neutralization by Mouse IL‑3 Antibody. Recombinant Mouse IL-3 (Catalog # 403-ML) stimulates proliferation in the NFS60 mouse myeloid cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-3 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-3 Polyclonal Antibody (Catalog # AB-403-NA). The ND₅₀ is typically 0.015-0.025 µg/mL.

Preparation and Storage

Reconstitution

Reconstitute at 1 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-3

Interleukin 3 is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. In addition, IL-3 also affects the functional activity of mature mast cells, basophils, eosinophils and macrophages. Because of its multiple functions and targets, it was originally studied under different names, including mast cell growth factor P-cell stimulating factor, burst promoting activity, multi-colony stimulating factor, thy-1 inducing factor and WEHI-3 growth factor. In addition to activated T cells, other cell types such as human thymic epithelial cells, activated mouse mast cells, mouse keratinocytes and neurons/astrocytes can also produce IL-3. At the amino acid sequence level, mature human and mouse IL-3 share only 29% sequence identity. Consistent with this lack of homology, IL-3 activity is highly species-specific and human IL-3 does not show activity on mouse cells.

IL-3 exerts its biological activities through binding to specific cell surface receptors. The high affinity receptor responsible for IL-3 signaling is composed of alpha and beta subunits. The IL-3R alpha is a member of the cytokine receptor super family and binds IL-3 with low affinity. Two distinct beta subunits, AIC2A ( beta _IL-3) and AIC2B ( beta _c) are present in mouse cells. beta _IL-3 also binds IL-3 with low affinity and forms a high affinity receptor with the alpha subunit. The beta _c subunits does not bind any cytokine but forms functional high affinity receptors with the alpha subunit of the IL-3, IL-5 and GM-CSF receptors. Receptors for IL-3 are present on bone marrow progenitors, macrophages, mast cells, eosinophils, megakaryocytes, basophils and various myeloid leukemic cells.

References

Yokota, T. et al., 1984, Proc. Natl. Acad. Sci. USA 81:1070.
Fung, M.C. et al., 1984, Nature 307:233.
Miyatake, S. et al., 1985, Proc. Natl. Acad. Sci. USA 82:316.

Long Name

Interleukin 3

Entrez Gene IDs

3562 (Human); 16187 (Mouse); 24495 (Rat)

Alternate Names

Hematopoietic growth factor; IL3; IL-3; IL-3MGC79398; interleukin 3 (colony-stimulating factor, multiple); interleukin-3; Mast cell growth factor; mast-cell growth factor; MCGF; MCGFMGC79399; MULTI-CSF; multilineage-colony-stimulating factor; Multipotential colony-stimulating factor; P-cell stimulating factor; P-cell-stimulating factor