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Mouse IL-23 p19 Antibody

Catalog #: AF1619
18 Citations Datasheet
Catalog # Availability Size / Price Qty
AF1619
AF1619-SP
IL‑17 secretion Induced by IL‑23 and Neutralization by Mouse IL‑23 Antibody.
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Citations (18)
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Mouse IL-23 p19 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-23 p19 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse IL‑12/23 p40 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse IL‑23 p19
Met1-Ala196
Accession # Q9EQ14
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse IL‑23 (Catalog # 1887-ML)
Neutralization
Measured by its ability to neutralize IL‑23-induced IL‑17 secretion in mouse splenocytes. Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910. The Neutralization Dose (ND50) is typically 0.07-0.35 µg/mL in the presence of 0.75 ng/mL Recombinant Mouse IL‑23 and 10 ng/mL Recombinant Mouse IL‑2.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization IL‑17 secretion Induced by IL‑23 and Neutralization by Mouse IL‑23 Antibody. View Larger

IL‑17 secretion Induced by IL‑23 and Neutralization by Mouse IL‑23 Antibody. In the presence of Recombinant Mouse IL-2 (10 ng/mL, Catalog # 402-ML), Recombinant Mouse IL-23 (Catalog # 1887-ML) stimulates IL-17 secretion in mouse splenocytes in a dose-dependent manner (orange line), as measured by the Mouse IL-17 Quantikine ELISA Kit (Catalog # M1700). Under these conditions, IL-17 secretion elicited by Recombinant Mouse IL-23 (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL-23 p19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1619). The ND50 is typically 0.07-0.35 µg/mL.

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-23

Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12 (1-5). The p19 subunit has homology to the p35 subunit of IL-12, as well as to other single chain cytokines such as IL-6 and IL-11. The p40 subunit is homologous to the extracellular domains of the hematopoietic cytokine receptors. Mouse p19 cDNA encodes a 196 amino acid (aa) residue precursor protein with a putative 19 aa signal peptide and 177 aa mature protein. Human and mouse p19 share 70% aa sequence identity. Although p19 is expressed by activated macrophages, dendritic cells, T cells, and endothelial cells, only activated macrophages and dendritic cells express p40 concurrently to produce IL-23. The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12 R beta 1) and the IL-23-specific receptor subunit (IL-23 R). IL-23 has biological activities that are similar to, but distinct from IL-12. Both IL-12 and IL-23 induce proliferation and IFN-gamma production by human T cells. While IL-12 acts on both naïve and memory human T cells, the effects of IL-23 is restricted to memory T cells. In mouse, IL-23 but not IL-12, has also been shown to induce memory T cells to secret IL-17, a potent proinflammatory cytokine. IL-12 and IL-23 can induce IL-12 production from mouse splenic DC of both the CD8- and CD8+ subtypes, however only IL-23 can act directly on CD8+ DC to mediate immunogenic presentation of poorly immunogenic tumor/self peptide.

References
  1. Oppmann, B. et al. (2000) Immunity 13:715.
  2. Lankford, C.S. and D.M. Frucht (2003) J. Leukoc. Biol. 73:49.
  3. Parham, C. et al. (2002) J. Immunol. 168:5699.
  4. Belladonna, M.L. et al. (2002) J. Immunol. 168:5448.
  5. Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910.
Long Name
Interleukin 23
Entrez Gene IDs
51561 (Human); 83430 (Mouse); 155140 (Rat); 102128555 (Cynomolgus Monkey)
Alternate Names
IL-23 p19/IL-12 p40; IL23; IL-23; IL-23A; IL-23-A; IL-23p19; IL-23p19/IL-12p40; IL23P19P19; interleukin 23 p19 subunit; interleukin 23, alpha subunit p19; interleukin-23 subunit alpha; Interleukin-23 subunit p19; JKA3 induced upon T-cell activation; MGC79388; SGRF; SGRFIL-23 subunit alpha

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Citations for Mouse IL-23 p19 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

18 Citations: Showing 1 - 10
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  1. T cell-directed IL-17 production by lung granular gamma δ T cells is coordinated by a novel IL-2 and IL-1 beta circuit
    Authors: Antoine Ménoret, James A. Buturla, Maria M. Xu, Julia Svedova, Sanjeev Kumar, Vijay A. K. Rathinam et al.
    Mucosal Immunology
  2. IL-17A Produced by Innate Lymphoid Cells Is Essential for Intestinal Ischemia-Reperfusion Injury
    Authors: M Geha, MG Tsokos, RE Bosse, T Sannikova, Y Iwakura, JJ Dalle Lucc, R De Waal Ma, GC Tsokos
    J. Immunol., 2017-09-06;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  3. Regulation of IL-12p40 by HIF controls Th1/Th17 responses to prevent mucosal inflammation
    Authors: E Marks, C Naudin, G Nolan, BJ Goggins, G Burns, SW Mateer, JK Latimore, K Minahan, M Plank, PS Foster, R Callister, M Veysey, MM Walker, NJ Talley, G Radford-Sm, S Keely
    Mucosal Immunol, 2017-01-25;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  4. Dendritic Cells in Irradiated Mice Trigger the Functional Plasticity and Antitumor Activity of Adoptively Transferred Tc17 Cells via IL12 Signaling.
    Authors: Bowers J, Nelson M, Kundimi S, Bailey S, Huff L, Schwartz K, Cole D, Rubinstein M, Paulos C
    Clin Cancer Res, 2015-04-22;21(11):2546-57.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  5. Tristetraprolin regulation of interleukin 23 mRNA stability prevents a spontaneous inflammatory disease
    Authors: Céline Molle, Tong Zhang, Laure Ysebrant de Lendonck, Cyril Gueydan, Mathieu Andrianne, Félicie Sherer et al.
    Journal of Experimental Medicine
  6. Dectin-1-dependent interleukin-22 contributes to early innate lung defense against Aspergillus fumigatus.
    Authors: Gessner MA, Werner JL, Lilly LM, Nelson MP, Metz AE, Dunaway CW, Chan YR, Ouyang W, Brown GD, Weaver CT, Steele C
    Infect. Immun., 2011-10-28;80(1):410-7.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  7. The encephalitogenicity of T(H)17 cells is dependent on IL-1- and IL-23-induced production of the cytokine GM-CSF.
    Authors: El-Behi M, Ciric B, Dai H
    Nat. Immunol., 2011-04-24;12(6):568-75.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  8. Contribution of IL-17-producing gamma delta T cells to the efficacy of anticancer chemotherapy.
    Authors: Ma Y, Aymeric L, Locher C, Mattarollo SR, Delahaye NF, Pereira P, Boucontet L, Apetoh L, Ghiringhelli F, Casares N, Lasarte JJ, Matsuzaki G, Ikuta K, Ryffel B, Benlagha K, Tesniere A, Ibrahim N, Dechanet-Merville J, Chaput N, Smyth MJ, Kroemer G, Zitvogel L
    J. Exp. Med., 2011-03-07;208(3):491-503.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  9. RORgammat+ innate lymphoid cells regulate intestinal homeostasis by integrating negative signals from the symbiotic microbiota.
    Authors: Sawa S, Lochner M, Satoh-Takayama N, Dulauroy S, Berard M, Kleinschek M, Cua D, Di Santo JP, Eberl G
    Nat. Immunol., 2011-02-20;12(4):320-6.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  10. The C5a receptor impairs IL-12-dependent clearance of Porphyromonas gingivalis and is required for induction of periodontal bone loss.
    Authors: Liang S, Krauss JL, Domon H, McIntosh ML, Hosur KB, Qu H, Li F, Tzekou A, Lambris JD, Hajishengallis G
    J. Immunol., 2010-12-13;186(2):869-77.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  11. Crucial role of interleukin-7 in T helper type 17 survival and expansion in autoimmune disease.
    Authors: Liu X, Leung S, Wang C, Tan Z, Wang J, Guo TB, Fang L, Zhao Y, Wan B, Qin X, Lu L, Li R, Pan H, Song M, Liu A, Hong J, Lu H, Zhang JZ
    Nat. Med., 2010-01-10;16(2):191-7.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  12. Prostaglandin mediates IL-23/IL-17-induced neutrophil migration in inflammation by inhibiting IL-12 and IFNgamma production.
    Authors: Lemos HP, Grespan R, Vieira SM, Cunha TM, Verri WA, Fernandes KS, Souto FO, McInnes IB, Ferreira SH, Liew FY, Cunha FQ
    Proc. Natl. Acad. Sci. U.S.A., 2009-03-16;106(14):5954-9.
    Species: Mouse
    Sample Types: In Vivo, Whole Cells
    Applications: Functional Assay, Neutralization
  13. Tumor-specific Th17-polarized cells eradicate large established melanoma.
    Authors: Muranski P, Boni A, Antony PA, Cassard L, Irvine KR, Kaiser A, Paulos CM, Palmer DC, Touloukian CE, Ptak K, Gattinoni L, Wrzesinski C, Hinrichs CS, Kerstann KW, Feigenbaum L, Chan CC, Restifo NP
    Blood, 2008-03-19;112(2):362-73.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  14. Lack of Toll IL-1R8 exacerbates Th17 cell responses in fungal infection.
    Authors: Bozza S, Zelante T, Moretti S, Bonifazi P, DeLuca A, D'Angelo C, Giovannini G, Garlanda C, Boon L, Bistoni F, Puccetti P, Mantovani A, Romani L
    J. Immunol., 2008-03-15;180(6):4022-31.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  15. Epicutaneous antigen exposure induces a Th17 response that drives airway inflammation after inhalation challenge.
    Authors: He R, Oyoshi MK, Jin H, Geha RS
    Proc. Natl. Acad. Sci. U.S.A., 2007-09-24;104(40):15817-22.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  16. Complement receptor 3 blockade promotes IL-12-Mediated Clearance of Porphyromonas gingivalis and Negates Its Virulence In Vivo.
    Authors: Hajishengallis G, Shakhatreh MA, Wang M, Liang S
    J. Immunol., 2007-08-15;179(4):2359-67.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  17. TLR3 and TLR7 are involved in expression of IL-23 subunits while TLR3 but not TLR7 is involved in expression of IFN-beta by Theiler's virus-infected RAW264.7 cells.
    Authors: Al-Salleeh F, Petro TM
    Microbes Infect., 2007-07-13;9(11):1384-92.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  18. T-bet regulates the fate of Th1 and Th17 lymphocytes in autoimmunity.
    Authors: Gocke AR, Cravens PD, Ben LH, Hussain RZ, Northrop SC, Racke MK, Lovett-Racke AE
    J. Immunol., 2007-02-01;178(3):1341-8.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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