Mouse IL-1ra/IL-1F3 Antibody Summary
Arg8-Gln159
Accession # Q542W1
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Mouse IL‑1ra/IL‑1F3 by Western Blot. Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line treated with 1 µg/mL LPS for 24 hours. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse IL-1ra/IL-1F3 Monoclonal Antibody (Catalog # MAB4801) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for IL-1ra/IL-1F3 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-1ra/IL-1F3
IL-1ra was originally isolated from the urine of patients with monocytic leukemia and has also been purified from adherent monocytes. The naturally-occurring, fully glycosylated form has an apparent molecular weight of about 25 kDa. The protein shows 26% amino acid homology to IL-1 beta and 19% homology to IL-1 alpha. It will compete with either factor for receptor binding, but does not interact with either one. Human IL-1ra will bind to both types of IL-1 receptor (I and II) on human cells. In mouse, IL-1 RII does not bind IL-1ra. The recombinant, non-glycosylated form of IL-1ra blocks binding of IL-1 to its receptor equally as well as the naturally-occurring, glycosylated form. The IL-1ra has been shown to block the inflammatory responses induced by IL-1 both in vitro and in vivo. Pre-clinical and clinical studies were done to test possible therapeutic applications for IL-1ra in the treatment of sepsis, rheumatoid arthritis and chronic myelogenous leukemia.
Product Datasheets
Citations for Mouse IL-1ra/IL-1F3 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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eIF4E-Binding Proteins 1 and 2 Limit Macrophage Anti-Inflammatory Responses through Translational Repression of IL-10 and Cyclooxygenase-2
Authors: M William, LP Leroux, V Chaparro, J Lorent, TE Graber, MN M'Boutchou, T Charpentie, A Fabié, CM Dozois, S Stäger, LC van Kempen, T Alain, O Larsson, M Jaramillo
J. Immunol., 2018-04-30;0(0):.
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A PROTEIN COMPLEX OF LIVER ORIGIN ACTIVATES A PRO-INFLAMMATORY PROGRAM THAT DRIVES HEPATIC AND INTESTINAL INJURY IN ALCOHOL-ASSOCIATED LIVER DISEASE
Authors: Ge, X;Han, H;Desert, R;Das, S;Song, Z;Komakula, SSB;Chen, W;Athavale, D;Lantvit, D;Nieto, N;
Cellular and molecular gastroenterology and hepatology
Species: Transgenic Mouse
Sample Types: Whole Cells
Applications: Functional Assay -
Extracellular microvesicles that originated adipose tissue derived mesenchymal stem cells have the potential ability to improve rheumatoid arthritis on mice
Authors: K Tsujimaru, M Takanashi, K Sudo, A Ishikawa, S Mineo, S Ueda, K Kumagai, M Kuroda
Regenerative Therapy, 2020-12-09;15(0):305-311.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
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