Mouse IL-1 alpha /IL-1F1 Antibody Summary
Ser6-Ser161
Accession # Q62161
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Recombinant Mouse and Rat IL‑1 alpha /IL‑1F1 by Western Blot. Western blot shows 50 ng of Recombinant Mouse IL-1a/IL-1F1 (400-ML), Recombinant Human IL-1a/IL-1F1 (200-LA) and Recombinant Rat IL-1a/IL-1F1 (500-RL). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Mouse IL-1a/IL-1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-400-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). A specific band was detected for IL-1a/IL-1F1 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
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IL‑1 alpha /IL‑1F1 in Mouse Liver. IL-1a/IL-1F1 was detected in perfusion fixed frozen sections of mouse liver using Goat Anti-Mouse IL-1a/IL-1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-400-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
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Cell Proliferation Induced by IL‑1 alpha /IL‑1F1 and Neutral-ization by Mouse IL‑1 alpha /IL‑1F1 Antibody. Recombinant Mouse IL-1a/IL-1F1 (400-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-1a/IL-1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-1a/IL-1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-400-NA). The ND50 is typically 0.002‑0.004 µg/mL.
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Detection of IL‑1 alpha /IL‑1F1 in Mouse Liver. IL‑1 alpha /IL‑1F1 was detected in immersion fixed paraffin-embedded sections of Mouse Liver using Goat Anti-Mouse IL‑1 alpha /IL‑1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-400-NA) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in hepatocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
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Detection of Mouse IL-1 alpha/IL-1F1 by Immunocytochemistry/ Immunofluorescence Experimental stroke-induced IL-1 expression is increased in the brain after LPS administration and anaphylaxis. Quantification of IL-1 alpha -positive cells (A) and granulocytes (B) in the ipsilateral hemisphere. C. Immunofluorescence shows IL-1 alpha -positive cells (green), granulocytes (red) and tomato lectin-positive blood vessels (blue) and microglia/macrophage cells in the ipsilateral cerebral cortex and thalamus after 24 h reperfusion. Perivascular cells in the cerebral cortex (i) and parenchymal cells in the thalamus (ii) expressing IL-1 alpha after MCAo are abundant in mice with anaphylaxis and LPS treatment, respectively. D. In situ hybridization reveals increased number of cells expressing IL-1 beta mRNA in the ischaemic cerebral cortex of mice 24 h after MCAo with anaphylaxis (Ova+A) compared to MCAo and Ova sensitization (Ova). E. Silver grains indicate the presence of IL-1 beta mRNA in Ova and Ova+A mice after MCAo (counterstained with cresyl violet). Inserts show higher magnification of IL-1 beta mRNA containing cells indicated by arrowheads. Data are representative of 4-5 mice in each group. One-way ANOVA followed by Bonferroni's comparison (P < 0.05 vs * Control, # Ova, $ Ova+A). Scale bars: Cii - 20 μm, C - 50 μm, E - 100 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/22114895), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Mouse IL-1 alpha/IL-1F1 by Immunocytochemistry/ Immunofluorescence Experimental stroke-induced IL-1 expression is increased in the brain after LPS administration and anaphylaxis. Quantification of IL-1 alpha -positive cells (A) and granulocytes (B) in the ipsilateral hemisphere. C. Immunofluorescence shows IL-1 alpha -positive cells (green), granulocytes (red) and tomato lectin-positive blood vessels (blue) and microglia/macrophage cells in the ipsilateral cerebral cortex and thalamus after 24 h reperfusion. Perivascular cells in the cerebral cortex (i) and parenchymal cells in the thalamus (ii) expressing IL-1 alpha after MCAo are abundant in mice with anaphylaxis and LPS treatment, respectively. D. In situ hybridization reveals increased number of cells expressing IL-1 beta mRNA in the ischaemic cerebral cortex of mice 24 h after MCAo with anaphylaxis (Ova+A) compared to MCAo and Ova sensitization (Ova). E. Silver grains indicate the presence of IL-1 beta mRNA in Ova and Ova+A mice after MCAo (counterstained with cresyl violet). Inserts show higher magnification of IL-1 beta mRNA containing cells indicated by arrowheads. Data are representative of 4-5 mice in each group. One-way ANOVA followed by Bonferroni's comparison (P < 0.05 vs * Control, # Ova, $ Ova+A). Scale bars: Cii - 20 μm, C - 50 μm, E - 100 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/22114895), licensed under a CC-BY license. Not internally tested by R&D Systems.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-1 alpha/IL-1F1
IL-1 is a name that designates two proteins, IL-1 alpha and IL-1 beta, that are the products of distinct genes, but recognize the same cell surface receptors. IL-1 alpha and IL-1 beta are structurally related polypeptides that show approximately 25% homology at the amino acid level. Both proteins are produced by a wide variety of cells in response to stimuli such as those produced by inflammatory agents, infections, or microbial endotoxins. The proteins are synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17.5 kDa. The specific protease responsible for the processing of IL-beta, designated interleukin 1 beta -converting enzyme (ICE), has been described. Mature human and mouse IL-1 beta share approximately 75% amino acid sequence identity and human IL-1 beta has been found to be active on murine cell lines.
IL-1 alpha and IL-1 beta are potent pro-inflammatory cytokines that induce a wide variety of biological activities on different cell types. Two distinct types of IL-1 receptors have been identified and cloned from human and mouse cells. The IL-1 receptor type I is a 80 kDa transmembrane protein with demonstrated IL-1 signaling function. The IL-1 receptor type II is a 68 kDa membrane protein with a relatively short cytoplasmic tail and has no signaling function. The type II receptor acts as a decoy target for IL-1, inhibiting IL-1 activities by preventing the binding of IL-1 to the type I receptor. A soluble version of the type II receptor is induced by anti‑inflammatory agents such as glucocorticoids, IL-4 and IL-13.
Product Datasheets
Citations for Mouse IL-1 alpha /IL-1F1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
31
Citations: Showing 1 - 10
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T. gondii infection induces IL-1R dependent chronic cachexia and perivascular fibrosis in the liver and skeletal muscle.
Authors: Melchor, S J, Hatter, J A Et al.
Sci Rep
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Spatiotemporally resolved colorectal oncogenesis in mini-colons ex vivo
Authors: Lorenzo-Martín, LF;Hübscher, T;Bowler, AD;Broguiere, N;Langer, J;Tillard, L;Nikolaev, M;Radtke, F;Lutolf, MP;
Nature
Species: Transgenic Mouse
Sample Types: Organoid
Applications: Immunohistochemistry -
Toll-like receptor 2 orchestrates a tumor suppressor response in non-small cell lung cancer
Authors: FR Millar, A Pennycuick, M Muir, A Quintanill, P Hari, E Freyer, P Gautier, A Meynert, G Grimes, CS Coll, S Zdral, S Victorelli, VH Teixeira, J Connelly, JF Passos, MA Ros, WAH Wallace, MC Frame, AH Sims, L Boulter, SM Janes, S Wilkinson, JC Acosta
Cell Reports, 2022-11-08;41(6):111596.
Species: Transgenic Mouse
Sample Types: Whole Tissue
Applications: IHC -
The alarmin interleukin-1? triggers secondary degeneration through reactive astrocytes and endothelium after spinal cord injury
Authors: Bretheau F, Castellanos-Molina A, B�langer D et al.
Nature Communications
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Itaconate and fumarate derivatives inhibit priming and activation of the canonical NLRP3 inflammasome in macrophages
Authors: Christopher Hoyle, Jack P. Green, Stuart M. Allan, David Brough, Eloise Lemarchand
Immunology
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Augmented secretion of IL-1&alpha from mouse oral squamous cell carcinoma (OSCC) cells caused by serum deprivation and hypoxia promotes immune suppressive activity of mesenchymal stromal cells
Authors: A Matsunami, M Mizuno-Kam, H Kawaki, E Takayama, K Ueno, M Ando, H Morimoto-I, Y Muramatsu, S Sumitomo, N Kondoh
Journal of oral biosciences, 2021-06-18;0(0):.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: Functional Assay -
Gasdermin D mediates the maturation and release of IL-1&alpha downstream of inflammasomes
Authors: K Tsuchiya, S Hosojima, H Hara, H Kushiyama, MR Mahib, T Kinoshita, T Suda
Cell Reports, 2021-03-23;34(12):108887.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Macrophages potentiate STAT3 signaling in skeletal muscles and regulate pancreatic cancer cachexia
Authors: Surendra K. Shukla, Spas D. Markov, Kuldeep S. Attri, Enza Vernucci, Ryan J. King, Aneesha Dasgupta et al.
Cancer Letters
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Ptpn6 inhibits caspase-8- and Ripk3/Mlkl-dependent inflammation
Authors: M Speir, CJ Nowell, AA Chen, JA O'Donnell, IS Shamie, PR Lakin, AA D'Cruz, RO Braun, JJ Babon, RS Lewis, M Bliss-More, I Shlomovitz, S Wang, LH Cengia, AI Stoica, R Hakem, MA Kelliher, LA O'Reilly, H Patsiouras, KE Lawlor, E Weller, NE Lewis, AW Roberts, M Gerlic, BA Croker
Nat. Immunol., 2019-12-09;21(1):54-64.
Species: Mouse
Sample Types: Whole Cells
Applications: Western Blot -
Developmentally Regulated Innate Immune NF kappa B Signaling Mediates IL-1 alpha Expression in the Perinatal Murine Lung
Authors: Brittany Butler, Robyn De Dios, Leanna Nguyen, Sarah McKenna, Sankar Ghosh, Clyde J. Wright
Frontiers in Immunology
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The three cytokines IL-1?, IL-18, and IL-1? share related but distinct secretory routes
Authors: VS Tapia, MJD Daniels, P Palazón-Ri, M Dewhurst, NM Luheshi, J Rivers-Aut, J Green, E Redondo-Ca, P Kaldis, G Lopez-Cast, D Brough
J. Biol. Chem., 2019-04-02;294(21):8325-8335.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Tacrolimus interacts with voriconazole to reduce the severity of fungal keratitis by suppressing IFN-related inflammatory responses and concomitant FK506 and voriconazole treatment suppresses fungal keratitis
Authors: J Zhong, L Peng, B Wang, H Zhang, S Li, R Yang, Y Deng, H Huang, J Yuan
Mol. Vis., 2018-03-04;24(0):187-200.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
HpARI Protein Secreted by a Helminth Parasite Suppresses Interleukin-33
Authors: Megan Osbourn, Dinesh C. Soares, Francesco Vacca, E. Suzanne Cohen, Ian C. Scott, William F. Gregory et al.
Immunity
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TLR sensing of bacterial spore-associated RNA triggers host immune responses with detrimental effects
Authors: MK Choo, Y Sano, C Kim, K Yasuda, XD Li, X Lin, M Stenzel-Po, L Alexopoulo, S Ghosh, E Latz, IR Rifkin, ZJ Chen, GC Stewart, H Chong, JM Park
J. Exp. Med., 2017-04-11;214(5):1297-1311.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Interleukin-1 and estrogen protect against disseminating dentoalveolar infections
Authors: H Youssef, P Stashenko
Int J Oral Sci, 2017-03-01;9(1):16-23.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Sustained, localized salicylic acid delivery enhances diabetic bone regeneration via prolonged mitigation of inflammation
Authors: Weiling Yu, Stephan Bien-Aime, Marcelo Mattos, Sarah Alsadun, Keisuke Wada, Sarah Rogado et al.
Journal of Biomedical Materials Research Part A
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Caspase-1-independent IL-1 release mediates blister formation in autoantibody-induced tissue injury through modulation of endothelial adhesion molecules.
Authors: Sadeghi H, Lockmann A, Hund A, Samavedam U, Pipi E, Vafia K, Hauenschild E, Kalies K, Pas H, Jonkman M, Iwata H, Recke A, Schon M, Zillikens D, Schmidt E, Ludwig R
J Immunol, 2015-03-20;194(8):3656-63.
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay -
AIM2 and NLRC4 inflammasomes contribute with ASC to acute brain injury independently of NLRP3.
Authors: Denes A, Coutts G, Lenart N, Cruickshank S, Pelegrin P, Skinner J, Rothwell N, Allan S, Brough D
Proc Natl Acad Sci U S A, 2015-03-16;112(13):4050-5.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
IL-1alpha signaling is critical for leukocyte recruitment after pulmonary Aspergillus fumigatus challenge.
Authors: Caffrey A, Lehmann M, Zickovich J, Espinosa V, Shepardson K, Watschke C, Hilmer K, Thammahong A, Barker B, Rivera A, Cramer R, Obar J
PLoS Pathog, 2015-01-28;11(1):e1004625.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Dendritic cell IL-1alpha and IL-1beta are polyubiquitinated and degraded by the proteasome.
Authors: Ainscough J, Frank Gerberick G, Zahedi-Nejad M, Lopez-Castejon G, Brough D, Kimber I, Dearman R
J Biol Chem, 2014-11-04;289(51):35582-92.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Cytokine signaling by grafted neuroectodermal stem cells rescues motoneurons destined to die
Authors: Krisztián Pajer, Georg A. Feichtinger, Gábor Márton, Sonja Sabitzer, Dieter Klein, Heinz Redl et al.
Experimental Neurology
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Interleukin-1 deficiency prolongs ovarian lifespan in mice.
Authors: Uri-Belapolsky S, Shaish A, Eliyahu E, Grossman H, Levi M, Chuderland D, Ninio-Many L, Hasky N, Shashar D, Almog T, Kandel-Kfir M, Harats D, Shalgi R, Kamari Y
Proc Natl Acad Sci U S A, 2014-08-11;111(34):12492-7.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
The frustrated host response to Legionella pneumophila is bypassed by MyD88-dependent translation of pro-inflammatory cytokines.
Authors: Asrat S, Dugan A, Isberg R
PLoS Pathog, 2014-07-24;10(7):e1004229.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
I kappa B epsilon Is a Key Regulator of B Cell Expansion by Providing Negative Feedback on cRel and RelA in a Stimulus-Specific Manner
Authors: Bryce N. Alves, Rachel Tsui, Jonathan Almaden, Maxim N. Shokhirev, Jeremy Davis-Turak, Jessica Fujimoto et al.
The Journal of Immunology
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Genetic and cellular evidence of decreased inflammation associated with reduced incidence of posttraumatic arthritis in MRL/MpJ mice
Authors: John S. Lewis, Bridgette D. Furman, Evan Zeitler, Janet L. Huebner, Virginia B. Kraus, Farshid Guilak et al.
Arthritis & Rheumatism
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Interleukin-1 alpha expression precedes IL-1 beta after ischemic brain injury and is localised to areas of focal neuronal loss and penumbral tissues
Authors: Nadia M Luheshi, Krisztina J Kovács, Gloria Lopez-Castejon, David Brough, Adam Denes
Journal of Neuroinflammation
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Systemic inflammatory challenges compromise survival after experimental stroke via augmenting brain inflammation, blood- brain barrier damage and brain oedema independently of infarct size
Authors: Ádám Dénes, Szilamér Ferenczi, Krisztina J Kovács
Journal of Neuroinflammation
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IL-1alpha and IL-1beta recruit different myeloid cells and promote different stages of sterile inflammation.
Authors: Rider P, Carmi Y, Guttman O, Braiman A, Cohen I, Voronov E, White MR, Dinarello CA, Apte RN
J. Immunol., 2011-09-19;187(9):4835-43.
Species: Mouse
Sample Types: Cell Lysates, In Vivo, Whole Cells, Whole Tissue
Applications: ICC, IHC-Fr, Neutralization, Western Blot -
Activation of the NLRP3 inflammasome by islet amyloid polypeptide provides a mechanism for enhanced IL-1beta in type 2 diabetes.
Authors: Masters SL, Dunne A, Subramanian SL, Hull RL, Tannahill GM, Sharp FA, Becker C, Franchi L, Yoshihara E, Chen Z, Mullooly N, Mielke LA, Harris J, Coll RC, Mills KH, Mok KH, Newsholme P, Nunez G, Yodoi J, Kahn SE, Lavelle EC, O'Neill LA
Nat. Immunol., 2010-09-12;11(10):897-904.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Comparison of the roles of IL-1, IL-6, and TNFalpha in cell culture and murine models of aseptic loosening.
Authors: Taki N, Tatro JM, Lowe R, Goldberg VM, Greenfield EM
Bone, 2006-12-21;40(5):1276-83.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization -
Membrane-bound Interleukin-1 alpha mediates leukocyte adhesion during atherogenesis
Species: Mouse
Sample Types: Cell Fractions
Applications: Western Blot
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