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Mouse IL-1 alpha /IL-1F1 Antibody

Catalog #: MAB4001
5 Citations Datasheet
Catalog # Availability Size / Price Qty
MAB4001
MAB4001-SP
Cell Proliferation Induced by IL‑1 alpha /IL‑1F1 and Neutralization by Mouse IL‑1 alpha /IL‑1F1 Antibody.
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Citations (5)
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Mouse IL-1 alpha /IL-1F1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-1 alpha in direct ELISAs and Western blots. In direct ELISAs, 100% and 30% cross-reactivity was observed with cotton rat IL-1 alpha and rat IL-1 alpha, respectively, and no cross-reactivity with recombinant human (rh) IL-1 alpha, rpIL-1 alpha, rmIL-1 beta, or rmIL-18 was observed.
Source
Monoclonal Hamster IgG Clone # ALF161
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse IL-1 alpha
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse IL‑1 alpha /IL‑1F1 (Catalog # 400-ML)
Immunoprecipitation
Fuhlbrigge, R. et al. (1988) J. Immunol. 141:2643.
 
Neutralization
Measured by its ability to neutralize IL‑1 alpha /IL‑1F1-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J. A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M. J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND50) is typically 0.005‑0.02 µg/mL in the presence of 50 pg/mL Recombinant Mouse IL‑1 alpha /IL‑1F1.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by IL‑1 alpha /IL‑1F1 and Neutralization by Mouse IL‑1 alpha /IL‑1F1 Antibody. View Larger

Cell Proliferation Induced by IL‑1 alpha /IL‑1F1 and Neutralization by Mouse IL‑1 alpha /IL‑1F1 Antibody. Recombinant Mouse IL-1a/IL-1F1 (Catalog # 400-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-1a/IL-1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Mouse IL-1a/IL-1F1 Monoclonal Antibody (Catalog # MAB4001). The ND50 is typically 0.005-0.02 µg/mL.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-1 alpha/IL-1F1

IL-1 is a name that designates two proteins, IL-1 alpha  and IL-1 beta, that are the products of distinct genes, but recognize the same cell surface receptors. IL-1 alpha and IL-1 beta are structurally related polypeptides that show approximately 25% homology at the amino acid level. Both proteins are produced by a wide variety of cells in response to stimuli such as those produced by inflammatory agents, infections, or microbial endotoxins. The proteins are synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17.5 kDa. The specific protease responsible for the processing of IL-beta, designated interleukin 1 beta -converting enzyme (ICE), has been described. Mature human and mouse IL-1 beta share approximately 75% amino acid sequence identity and human IL-1 beta has been found to be active on murine cell lines.

IL-1 alpha and IL-1 beta are potent pro-inflammatory cytokines that induce a wide variety of biological activities on different cell types. Two distinct types of IL-1 receptors have been identified and cloned from human and mouse cells. The IL-1 receptor type I is a 80 kDa transmembrane protein with demonstrated IL-1 signaling function. The IL-1 receptor type II is a 68 kDa membrane protein with a relatively short cytoplasmic tail and has no signaling function. The type II receptor acts as a decoy target for IL-1, inhibiting IL-1 activities by preventing the binding of IL-1 to the type I receptor. A soluble version of the type II receptor is induced by anti-inflammatory agents such as glucocorticoids, IL-4 and IL-13.

Long Name
Interleukin 1 alpha
Entrez Gene IDs
3552 (Human); 16175 (Mouse); 24493 (Rat); 397094 (Porcine); 101926546 (Cynomolgus Monkey)
Alternate Names
BAF; Hematopoietin-1; IL1 alpha; IL-1 alpha; IL1; IL1A; IL-1A; IL1-ALPHA; IL1F1; IL-1F1; IL1F1hematopoietin-1; interleukin 1, alpha; interleukin-1 alpha; LAF; LEM; preinterleukin 1 alpha; pro-interleukin-1-alpha

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Citations for Mouse IL-1 alpha /IL-1F1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Hypoxia promotes an inflammatory phenotype of fibroblasts in pancreatic cancer
    Authors: AM Mello, T Ngodup, Y Lee, KL Donahue, J Li, A Rao, ES Carpenter, HC Crawford, M Pasca di M, KE Lee
    Oncogenesis, 2022-09-15;11(1):56.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  2. Cigarette smoke-induced accumulation of lung dendritic cells is interleukin-1alpha-dependent in mice.
    Authors: Botelho F, Nikota J, Bauer C, Morissette M, Iwakura Y, Kolbeck R, Finch D, Humbles A, Stampfli M
    Respir Res, 2012-09-19;13(0):81.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  3. IL-1alpha/IL-1R1 expression in chronic obstructive pulmonary disease and mechanistic relevance to smoke-induced neutrophilia in mice.
    Authors: Botelho F, Bauer C, Finch D, Nikota J, Zavitz C, Kelly A, Lambert K, Piper S, Foster M, Goldring J, Wedzicha J, Bassett J, Bramson J, Iwakura Y, Sleeman M, Kolbeck R, Coyle A, Humbles A, Stampfli M
    PLoS ONE, 2011-12-06;6(12):e28457.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  4. IL-1-induced JAK/STAT signaling is antagonized by TGF-beta to shape CAF heterogeneity in pancreatic ductal adenocarcinoma.
    Authors: Giulia Biffi, Tobiloba E. Oni, Benjamin Spielman, Yuan Hao, Ela Elyada, Youngkyu Park et al.
    Cancer Discovery
  5. Ex vivo culture of mouse skin activates an interleukin 1 alpha‐dependent inflammatory response
    Authors: Hong‐Ming Zhou, Radomir M. Slominski, Leroy J. Seymour, Maria C. Bell, Priya Dave, Joseph Atumonye et al.
    Experimental Dermatology

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