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Mouse IL-17A/F Heterodimer Quantikine ELISA Kit

Catalog #: M17AF0
6 Citations Datasheet
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M17AF0
Mouse IL-17A/F Heterodimer ELISA Standard Curve
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Citations (6)
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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Mouse IL-17A/F Heterodimer Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Sensitivity
2.8 pg/mL
Assay Range
7.8 - 500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant mouse IL-17A/F heterodimer
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse IL-17A/F Heterodimer immunoassay is a 4.5 hour solid-phase ELISA designed to measure IL-17A/F heterodimer in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse mature IL-17A/F heterodimer and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse IL-17A/F heterodimer. Results obtained using natural mouse IL-17A/F heterodimer showed dose response curves that were parallel to the standard curves obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-17A/F heterodimer.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 22.8 119 180 25.1 112 188
Standard Deviation 1.4 5 11.1 2 9.8 11.6
CV% 6.1 4.2 6.2 8 8.8 6.2

Recovery

The recovery of mouse IL-17A/F heterodimer spiked to three levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Samples (n=4) 105 93-120
EDTA Plasma (n=4) 99 90-104
Heparin Plasma (n=4) 91 86-98
Serum (n=4) 91 84-98

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of recombinant mouse IL-17A/F heterodimer in each matrix were diluted with Calibrator Diluent and assayed.
Mouse IL-17A/F Heterodimer ELISA Linearity

Scientific Data

N/A Mouse IL-17A/F Heterodimer ELISA Standard Curve View Larger

Mouse IL-17A/F Heterodimer ELISA Standard Curve

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Product Datasheet

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-17A/F Heterodimer

The IL-17 family is comprised of at least six proinflammatory cytokines that share a conserved cysteine-knot structure but diverge at the N-terminus. IL-17 family members are glycoproteins secreted as dimers that induce local cytokine production and recruit granulocytes to sites of inflammation. IL-17 is induced by IL-15 and IL-23, mainly in activated CD4+ T cells distinct from Th1 or Th2 cells. IL-17F is the most homologous to IL-17, but is induced only by IL-23 in activated monocytes. IL-17B binds the IL-17B receptor, but not the IL-17 receptor; it is most homologous with IL-17D, which is expressed by resting CD4+ T cells and CD19+ B cells. IL-17E is mainly produced by Th2 cells and recruits eosinophils to lung tissue. IL-17C has a very restricted expression pattern but has been detected in adult prostate and fetal kidney libraries.

Long Name:
Interleukin 17 A/F
Alternate Names:
CTLA8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; Cytotoxic T-Lymphocyte-Associated Protein 8; IL17; IL-17; IL-17A; IL-17A/F Heterodimer; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A; interleukin-17A
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 50 µL Assay Diluent
  3.   Add 50 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
    6. 100 µL Conjugate
  6.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate and wash 5 times.
    8. 100 µL Substrate Solution
  8.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    9. 100 µL Stop Solution
  9.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Mouse IL-17A/F Heterodimer Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Lipoteichoic Acid from Lacticaseibacillus rhamnosus GG Modulates Dendritic Cells and T Cells in the Gut
    Authors: AD Friedrich, J Leoni, ML Paz, DH González M
    Nutrients, 2022-02-08;14(3):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  2. Epac-2 ameliorates spontaneous colitis in Il-10-/- mice by protecting the intestinal barrier and suppressing NF-kappaB/MAPK signalling
    Authors: X Song, H Wen, L Zuo, Z Geng, J Nian, L Wang, Y Jiang, J Tao, Z Zhu, X Wu, Z Wang, X Zhang, L Yu, H Zhao, P Xiang, J Li, L Shen, J Hu
    Journal of Cellular and Molecular Medicine, 2021-12-03;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  3. V&gamma4 T cell-derived IL-17A is essential for amplification of inflammatory cascades in ischemic brain tissue after stroke
    Authors: L Lu, Y Wang, L Zhou, Y Li, X Zhang, X Hu, S Shi, W He
    International immunopharmacology, 2021-05-24;96(0):107678.
    Species: Mouse
    Sample Types: Tissue Homogenates
  4. The YAP/HIF-1&alpha/miR-182/EGR2 axis is implicated in asthma severity through the control of Th17 cell differentiation
    Authors: J Zhou, N Zhang, W Zhang, C Lu, F Xu
    Cell & bioscience, 2021-05-12;11(1):84.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  5. Huagan tongluo Fang improves liver fibrosis via down-regulating miR-184 and up-regulating FOXO1 to inhibit Th17 cell differentiation
    Authors: J Xuan, A Huang, D Hu, J Geng, Y Tian, Z Cheng, Y Qiu
    Exp. Mol. Pathol., 2020-05-04;115(0):104447.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  6. Endogenous n-3 polyunsaturated fatty acids protect against imiquimod-induced psoriasis-like inflammation via the IL-17/IL-23 axis.
    Authors: Qin S, Wen J, Bai X, Chen T, Zheng R, Zhou G, Ma J, Feng J, Zhong B, Li Y
    Mol Med Rep, 2014-04-09;9(6):2097-104.
    Species: Mouse
    Sample Types: Serum

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