Mouse IL-17 Quantikine HS ELISA Kit

Catalog #: MHS170 Datasheet

Catalog #	Availability	Size / Price	Qty
MHS170

Control Products Available

QC292: Quantikine Immunoassay Control 1083 for Mouse IL-17 HS

Mouse IL-17 Quantikine HS ELISA Standard Curve

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All IL-17/IL-17A ELISAs

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Mouse IL-17 Quantikine HS ELISA Kit Summary

Assay Length

4.0 hours

Sample Type & Volume Required Per Well

Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)

Sensitivity

0.214 pg/mL

Assay Range

0.8 - 50 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine™ HS Mouse IL-17 Immunoassay is a 4.0 hour solid phase ELISA designed to measure mouse IL-17 levels in serum and plasma. It contains E. coli-expressed recombinant mouse IL-17 and antibodies raised against the recombinant protein. Results obtained using natural IL-17 showed linear curves that were parallel to the standard curves obtained using the Quantikine HS kit standards. These results indicate that the Quantikine HS Immunoassay kit can be used to determine relative mass values for natural mouse IL-17.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	6.82	16.9	31.9	6.92	16.6	31.4
Standard Deviation	0.239	0.429	0.836	0.349	0.814	1.64
CV%	3.5	2.5	2.6	5.1	4.9	5.2

Recovery

The recovery of mouse IL-17 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	90	78-96
Heparin Plasma (n=4)	89	80-97
Serum (n=4)	89	75-98

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of mouse IL-17 were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

Scientific Data

N/A

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Mouse IL-17 Quantikine HS ELISA Standard Curve

Product Datasheets

Product Datasheet

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-17/IL-17A

The IL-17 family is comprised of at least six proinflammatory cytokines that share a conserved cysteine-knot structure but diverge at the N-terminus. IL-17 family members are glycoproteins secreted as dimers that induce local cytokine production and recruit granulocytes to sites of inflammation. IL-17 is induced by IL-15 and IL-23, mainly in activated CD4+ T cells distinct from Th1 or Th2 cells. IL-17F is the most homologous to IL-17, but is induced only by IL-23 in activated monocytes. IL-17B binds the IL-17B receptor, but not the IL-17 receptor; it is most homologous with IL-17D, which is expressed by resting CD4+ T cells and CD19+ B cells. IL-17E is mainly produced by Th2 cells and recruits eosinophils to lung tissue. IL-17C has a very restricted expression pattern but has been detected in adult prostate and fetal kidney libraries.

Long Name:

Interleukin 17

Entrez Gene IDs:

3605 (Human); 16171 (Mouse); 301289 (Rat); 449530 (Porcine); 481837 (Canine); 102119976 (Cynomolgus Monkey)

Alternate Names:

CTLA8; CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17; IL-17; IL17A; IL-17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; IL17interleukin-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A