Mouse IL-17/IL-17A PerCP-conjugated Antibody Summary
Thr22-Ala158
Accession # Q62386
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of IL‑17/IL‑17A in Mouse splenocytes by Flow Cytometry. Mouse splenocytes differentiated to Th17 cells with plate-bound Rat anti-mouse CD3 Monoclonal antibody (10 μg/ml; MAB484), Goat anti-mouse CD28 Polyclonal Antibody (5 μg/mL; AF483), TGF-beta (10 ng/mL; 100-B), Recombinant Mouse IL-23 (20 ng/mL; 1887-ML), Recombinant Mouse IL-6 (40 ng/mL; 406-ML), and Recombinant MouseIL-1 beta (10 ng/mL; 401-ML) for 6 days were stained with (A) Goat Anti-Mouse IL-17/IL-17A PerCP-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC421C) or (B) control antibody (IC108C) and Rat anti-Mouse CD4 PE-conjugated Monoclonal Antibody (FAB554P). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Kit (FC012). Staining was performed using our Staining Intracellular Molecules protocol.
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Detection of IL‑17/IL‑17A in EL‑4 Mouse Cell Line by Flow Cytometry. LPS activated EL-4 mouse lymphoblast cell line was stained with Goat Anti-Mouse IL-17/IL-17A PerCP-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC421C, filled histogram) or isotype control antibody (Catalog # IC108C, open histogram). Cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
Background: IL-17/IL-17A
Interleukin 17, also known as IL-17A and CTLA-8, was initially identified as a 17 kDa, secreted T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpesvirus Saimiri. Mouse IL-17 cDNA encodes a 158 amino acid (aa) residue precursor protein with a 25 amino acid residue signal peptide that is cleaved to yield the 133 aa residue mature IL-17. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. IL-17 is also known to form a heterodimer with IL-17F. At the amino acid level, mIL-17 shows 62% and 87% aa sequence identity with human and rat IL-17, respectively. The receptor for the IL-17A homodimer and IL-17A:F heterodimer is reported to be a combinationof IL-17 RA and IL-17 RC, with a possible contribution by IL-17 RD. The expression of IL-17 is widespread, and found associated with LTi cells, B cells, gamma δ T cells, CD4+ Th17 cells, iNKT cells, neutrophils, intestinal Paneth cells, Type I ILCs and CD8+ Tc17 cells. IL-17 exhibits multiple biological activities on a variety of cells including: the induction of IL-6 and IL-8 production in fibroblasts, the enhancement of surface expression of ICAM-1 in fibroblasts, activation of NF-kappa B and costimulation of T cell proliferation, the preservation of intestinal mucosal integrity, and the induction of antimicrobial peptides by epithelium.
Product Datasheets
Citation for Mouse IL-17/IL-17A PerCP-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Maternal high-fat diet results in microbiota-dependent expansion of ILC3s in mice offspring
Authors: ST Babu, X Niu, M Raetz, RC Savani, LV Hooper, J Mirpuri
JCI Insight, 2018-10-04;3(19):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
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