Home / IL-17/IL-17A / Mouse IL-17 ELISpot Kit

Mouse IL-17 ELISpot Kit

Catalog #: EL421
8 Citations Datasheet
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XEL421
EL421
R&D Systems ELISpot and FluoroSpot Kits
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Mouse IL-17 ELISpot Kit Summary

Assay Type
Quantitative Sandwich ELISA
Format
96-well PVDF-backed microplate
Assay Length
3 hours 15 mins to 4 hours 30 mins*
Sample Type
Whole Cells
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

PRODUCT SUMMARY
ELISpot kits are highly sensitive, microplate-based assays for the detection of cytokine secreting cells. This kit is designed for the detection and enumeration of mouse IL-17/IL-17A. Complete ELISpot kits are ready-to-run and require no assay development or refinement.

This ELISpot assay employs a capture antibody specific for mouse IL-17/IL-17A, pre-coated onto a PVDF-backed microplate. Appropriately stimulated cells are pipetted directly into the wells and the immobilized antibody in the immediate vicinity of the secreting cells binds secreted mouse IL-17/IL-17A. Following wash steps and incubation with a biotinylated detection antibody, alkaline-phosphatase conjugated to streptavidin is added. Unbound enzyme is subsequently removed by washing and a substrate solution (BCIP/NBT) is added. A blue-black colored precipitate forms at the sites of cytokine localization and appears as spots, with each individual spot representing an individual mouse IL-17/IL-17A secreting cell. The spots can be counted with an automated ELISpot reader system or manually using a stereomicroscope.

PRODUCT FEATURES

  • Detect and quantitate individual cells secreting mouse IL-17/IL-17A
  • High sensitivity - ELISpot assays can measure responses with frequencies well below 1 in 100,000 cells
  • No in vitro expansion of cells required
  • High-throughput - ELISpot assays use only a small number of primary cells

KIT CONTENTS

  • Mouse IL-17/IL-17A Microplate
  • Biotinylated Detection Antibody
  • Streptavidin conjugated to Alkaline Phosphatase
  • Dilution Buffers
  • Wash Buffer Concentrate
  • BCIP/NBT Chromogen
  • Mouse IL-17/IL-17A Positive Control

OTHER REAGENTS REQUIRED

  • Pipettes and pipette tips
  • Deionized or distilled water
  • Squirt bottle, manifold dispenser, or automated microplate washer
  • 500 mL graduated cylinder
  • 37 °C CO2 incubator
  • Sterile culture media
  • Dissection microscope or an automated ELISpot reader

Product Datasheets

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Product Datasheet

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-17/IL-17A

The IL-17 family is comprised of at least six proinflammatory cytokines that share a conserved cysteine-knot structure but diverge at the N-terminus. IL-17 family members are glycoproteins secreted as dimers that induce local cytokine production and recruit granulocytes to sites of inflammation. IL-17 is induced by IL-15 and IL-23, mainly in activated CD4+ T cells distinct from Th1 or Th2 cells. IL-17F is the most homologous to IL-17, but is induced only by IL-23 in activated monocytes. IL-17B binds the IL-17B receptor, but not the IL-17 receptor; it is most homologous with IL-17D, which is expressed by resting CD4+ T cells and CD19+ B cells. IL-17E is mainly produced by Th2 cells and recruits eosinophils to lung tissue. IL-17C has a very restricted expression pattern but has been detected in adult prostate and fetal kidney libraries.

Long Name:
Interleukin 17
Entrez Gene IDs:
3605 (Human); 16171 (Mouse); 301289 (Rat); 449530 (Porcine); 481837 (Canine); 102119976 (Cynomolgus Monkey)
Alternate Names:
CTLA8; CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17; IL-17; IL17A; IL-17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; IL17interleukin-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A
⚠ WARNING: This product can expose you to chemicals including methanol, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov.

Citations for Mouse IL-17 ELISpot Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Scavenger receptor-A is a biomarker and effector of rheumatoid arthritis: A large-scale multicenter study
    Authors: F Hu, X Jiang, C Guo, Y Li, S Chen, W Zhang, Y Du, P Wang, X Zheng, X Fang, X Li, J Song, Y Xie, F Huang, J Xue, M Bai, Y Jia, X Liu, L Ren, X Zhang, J Guo, H Pan, Y Su, H Yi, H Ye, D Zuo, J Li, H Wu, Y Wang, R Li, L Liu, XY Wang, Z Li
    Nat Commun, 2020-04-20;11(1):1911.
    Species: Mouse
    Sample Types: Whole Cells
  2. Broad and Effective Protection against Staphylococcus aureus Is Elicited by a Multivalent Vaccine Formulated with Novel Antigens
    Authors: J Deng, X Wang, BZ Zhang, P Gao, Q Lin, RY Kao, K Gustafsson, KY Yuen, JD Huang
    mSphere, 2019-09-04;4(5):.
    Species: Mouse
    Sample Types: Whole Cells
  3. The increased protection and pathology in Mycobacterium tuberculosis-infected IL-27R-alpha-deficient mice is supported by IL-17A and is associated with the IL-17A-induced expansion of multifunctional T cells
    Authors: H Erdmann, J Behrends, K Ritter, A Hölscher, J Volz, I Rosenkrand, C Hölscher
    Mucosal Immunol, 2018-05-04;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
  4. Elongated TCR alpha chain CDR3 favors an altered CD4 cytokine profile.
    Authors: Reynolds C, Chong D, Raynsford E, Quigley K, Kelly D, Llewellyn-Hughes J, Altmann D, Boyton R
    BMC Biol, 2014-05-09;12(0):32.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  5. Excretory/secretory products from Trichinella spiralis adult worms ameliorate DSS-induced colitis in mice.
    Authors: Yang, Xiaodi, Yang, Yaping, Wang, Yunyun, Zhan, Bin, Gu, Yuan, Cheng, Yuli, Zhu, Xinping
    PLoS ONE, 2014-05-02;9(5):e96454.
    Species: Mouse
    Sample Types: Cell Culture Supernates, Whole Cells
  6. The Shc family protein adaptor, Rai, acts as a negative regulator of Th17 and Th1 cell development.
    Authors: Savino, Maria Te, Ulivieri, Cristina, Emmi, Giacomo, Prisco, Domenico, De Falco, Giulia, Ortensi, Barbara, Beccastrini, Enrico, Emmi, Lorenzo, Pelicci, Giuliana, D'Elios, Mario M, Baldari, Cosima T
    J Leukoc Biol, 2013-01-23;93(4):549-59.
    Species: Mouse
    Sample Types: Whole Cells
  7. TH17 cells mediate steroid-resistant airway inflammation and airway hyperresponsiveness in mice.
    Authors: McKinley L, Alcorn JF, Peterson A, Dupont RB, Kapadia S, Logar A, Henry A, Irvin CG, Piganelli JD, Ray A, Kolls JK
    J. Immunol., 2008-09-15;181(6):4089-97.
    Species: Mouse
    Sample Types: Whole Cells
  8. Resistance to experimental autoimmune encephalomyelitis and impaired IL-17 production in protein kinase C theta-deficient mice.
    Authors: Tan SL, Zhao J, Bi C, Chen XC, Hepburn DL, Wang J, Sedgwick JD, Chintalacharuvu SR, Na S
    J. Immunol., 2006-03-01;176(5):2872-9.
    Species: Mouse
    Sample Types: Whole Cells

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