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Mouse IL-13 Antibody

Catalog #: MAB413
23 Citations 2 Reviews Datasheet
Catalog # Availability Size / Price Qty
MAB413-SP
MAB413-100
MAB413-500
Cell Proliferation Induced by IL‑13 and Neutralization by Mouse IL‑13 Antibody.
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Citations (23)
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Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IL-13 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-13 in ELISAs and Western blots. In ELISAs, less than 0.1% cross-reactivity with recombinant human IL‑13 is observed.
Source
Monoclonal Rat IgG2A Clone # 38213
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse IL-13
Accession # P20109
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse IL‑13 (Catalog # 413-ML)

Mouse IL-13 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Mouse IL‑13 Biotinylated Antibody (Catalog # BAF413)

Standard: Recombinant Mouse IL-13 Protein (Catalog # 413-ML)

Neutralization
Measured by its ability to neutralize IL‑13-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.3-0.8 µg/mL in the presence of 10 ng/mL Recombinant Mouse IL‑13.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by IL‑13 and Neutralization by Mouse IL‑13 Antibody. View Larger

Cell Proliferation Induced by IL‑13 and Neutralization by Mouse IL‑13 Antibody. Recombinant Mouse IL-13 (Catalog # 413-ML) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-13 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL-13 Monoclonal Antibody (Catalog # MAB413). The ND50 is typically 0.3-0.8 µg/mL.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-13

IL-13 is a 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergic asthma and atopy. It is secreted by Th1 and Th2 CD4+ T cells, NK cells, visceral smooth muscle cells, eosinophils, mast cells, and basophils (1-3). IL-13 circulates as a monomer with two internal disulfide bonds that contribute to a bundled four alpha -helix configuration (4, 5). Mature mouse IL-13 shares 57%, 75%, and 58% amino acid sequence identity with human, rat, and rhesus IL-13, respectively. Despite the low homology, it exhibits cross-species activity between human, mouse, and rat (6, 7). IL-13 has diverse activities on numerous cell types (8). On macrophages, IL-13 suppresses the production of pro-inflammatory cytokines and other cytotoxic substances. On B cells, IL-13 induces immunoglobulin class switching to IgE, upregulates the expression of MHC class II, CD71, CD72, and CD23, and costimulates proliferation. IL-13 upregulates IL-6 while downregulating IL-1 and TNF-alpha production by fibroblasts and endothelial cells. IL-13 binds with low affinity to IL-13 R alpha 1, triggering IL-13 R alpha 1 association with IL-4 R alpha. This high affinity receptor complex also functions as the type 2 IL-4 receptor complex (9, 10). Additionally, IL-13 binds with high affinity to IL-13 R alpha 2 which is expressed intracellularly, on the cell surface, and as a soluble molecule (11-14). IL-13 R alpha 2 regulates the bioavailability of both IL-13 and IL-4 and is overexpressed in glioma and several bronchial pathologies (10, 15, 16). Compared to wild type IL-13, the atopy-associated R110Q variant of IL-13 elicits increased responsiveness from eosinophils that express low levels of IL-13 R alpha 2 (17).

References
  1. Wills-Karp, M. (2004) Immunol. Rev. 202:175.
  2. Nakajima, H. and K. Takatsu (2007) Int. Arch. Allergy Immunol. 142:265.
  3. Brown, K.D. et al. (1989) J. Immunol. 142:679.
  4. Moy, F.J. et al. (2001) J. Mol. Biol. 310:219.
  5. Eisenmesser, E.Z. et al. (2001) J. Mol. Biol. 310:231.
  6. Ruetten, H. and C. Thiemermann (1997) Shock 8:409.
  7. Lakkis, F.G. et al. (1997) Biochem. Biophys. Res. Commun. 235:529.
  8. Wynn, T.A. (2003) Annu. Rev. Immunol. 21:425.
  9. Andrews, A.L. et al. (2002) J. Biol. Chem. 277:46073.
  10. Tabata, Y. et al. (2007) Curr. Allergy Asthma Rep. 7:338.
  11. Chiaramonte, M.G. et al. (2003) J. Exp. Med. 197:687.
  12. Daines, M.O. and G.K. Hershey (2002) J. Biol. Chem. 227:10387.
  13. Matsumura, M. et al. (2007) Biochem. Biophys. Res. Commun. 360:464.
  14. Tabata, Y. et al. (2007) J. Immunol. 177:7905.
  15. Andrews, A.L. et al. (2006) J. Allergy Clin. Immunol. 118:858.
  16. Joshi, B.H. et al. (2006) Vitam. Horm. 74:479.
  17. Andrews, A-L. et al. (2007) J. Allergy Clin. Immunol. 120:91.
Long Name
Interleukin 13
Entrez Gene IDs
3596 (Human); 16163 (Mouse); 116553 (Rat); 574325 (Primate)
Alternate Names
ALRHMGC116789; BHR1interleukin-13; IL13; IL-13; IL-13MGC116788; interleukin 13; MGC116786; NC30; P600

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Citations for Mouse IL-13 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

23 Citations: Showing 1 - 10
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  1. Time-dependent cell-state selection identifies transiently expressed genes regulating ILC2 activation
    Authors: Yumiko Tanaka, Mai Yamagishi, Yasutaka Motomura, Takashi Kamatani, Yusuke Oguchi, Nobutake Suzuki et al.
    Commun Biol
  2. Paneth Cell-Derived Lysozyme Defines the Composition of Mucolytic Microbiota and the Inflammatory Tone of the Intestine
    Authors: Shiyan Yu, Iyshwarya Balasubramanian, Daniel Laubitz, Kevin Tong, Sheila Bandyopadhyay, Xiang Lin et al.
    Immunity
  3. IGF-1 receptor signaling regulates type II pneumocyte senescence and resulting macrophage polarization in lung fibrosis.
    Authors: Eun Joo Chung, Seokjoo Kwon, Jessica L. Reedy, Ayla O. White, Joon Seon Song, Ilseon Hwang et al.
    International Journal of Radiation Oncology*Biology*Physics
  4. A tuft cell - ILC2 signaling circuit provides therapeutic targets to inhibit gastric metaplasia and tumor development
    Authors: O'Keefe, RN;Carli, ALE;Baloyan, D;Chisanga, D;Shi, W;Afshar-Sterle, S;Eissmann, MF;Poh, AR;Pal, B;Seillet, C;Locksley, RM;Ernst, M;Buchert, M;
    Nature communications
    Species: Mouse
    Sample Types: In Vivo, Organoids
    Applications: In Vivo, Neutralization
  5. Interleukin-9 promotes mast cell progenitor proliferation and CCR2-dependent mast cell migration in allergic airway inflammation
    Authors: Pajulas, A;Fu, Y;Cheung, CCL;Chu, M;Cannon, A;Alakhras, N;Zhang, J;Ulrich, BJ;Nelson, AS;Zhou, B;Kaplan, MH;
    Mucosal immunology
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  6. Sphingosine-1-phosphate receptor-2 facilitates pulmonary fibrosis through potentiating IL-13 pathway in macrophages
    Authors: J Zhao, Y Okamoto, Y Asano, K Ishimaru, S Aki, K Yoshioka, N Takuwa, T Wada, Y Inagaki, C Takahashi, T Nishiuchi, Y Takuwa
    PLoS ONE, 2018-05-21;13(5):e0197604.
    Species: Mouse
    Sample Types:
    Applications: Neutralization
  7. Macrophage migration inhibitory factor deficiency enhances immune response to Nippostrongylus brasiliensis
    Authors: SR Damle, RK Martin, JV Cross, DH Conrad
    Mucosal Immunol, 2016-04-06;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Capture)
  8. Combination of inflammation-related cytokines promotes long-term muscle stem cell expansion.
    Authors: Fu X, Xiao J, Wei Y, Li S, Liu Y, Yin J, Sun K, Sun H, Wang H, Zhang Z, Zhang B, Sheng C, Wang H, Hu P
    Cell Res, 2015-05-15;25(6):655-73.
    Species: Mouse
    Sample Types: Protein
    Applications: ELISA Development (Capture)
  9. RSV vaccine-enhanced disease is orchestrated by the combined actions of distinct CD4 T cell subsets.
    Authors: Knudson C, Hartwig S, Meyerholz D, Varga S
    PLoS Pathog, 2015-03-13;11(3):e1004757.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Capture)
  10. CD8(+) cells regulate the T helper-17 response in an experimental murine model of Sjogren syndrome.
    Authors: Zhang X, Schaumburg C, Coursey T, Siemasko K, Volpe E, Gandhi N, Li D, Niederkorn J, Stern M, Pflugfelder S, de Paiva C
    Mucosal Immunol, 2013-09-11;7(2):417-27.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ELISA Development (Capture)
  11. Breast milk immune complexes are potent inducers of oral tolerance in neonates and prevent asthma development.
    Authors: Mosconi E, Rekima A, Seitz-Polski B
    Mucosal Immunol, 2010-05-19;3(5):461-74.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  12. Chlamydial respiratory infection during allergen sensitization drives neutrophilic allergic airways disease.
    Authors: Horvat JC, Starkey MR, Kim RY, Beagley KW, Preston JA, Gibson PG, Foster PS, Hansbro PM
    J. Immunol., 2010-03-12;184(8):4159-69.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  13. IL-6 is required for airway mucus production induced by inhaled fungal allergens.
    Authors: Neveu WA, Allard JB, Dienz O, Wargo MJ, Ciliberto G, Whittaker LA, Rincon M
    J. Immunol., 2009-07-10;183(3):1732-8.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  14. FTY720 ameliorates oxazolone colitis in mice by directly affecting T helper type 2 functions.
    Authors: Daniel C, Sartory NA, Zahn N
    Mol. Immunol., 2007-05-01;44(13):3305-16.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  15. Loss of pro-apoptotic Bim promotes accumulation of pulmonary T lymphocytes and enhances allergen-induced goblet cell metaplasia.
    Authors: Pierce J, Rir-Sima-Ah J, Estrada I, Wilder J, Strasser A, Tesfaigzi Y
    Am. J. Physiol. Lung Cell Mol. Physiol., 2006-06-16;291(5):L862-70.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development
  16. Severe sepsis exacerbates cell-mediated immunity in the lung due to an altered dendritic cell cytokine profile.
    Authors: Wen H, Hogaboam CM, Gauldie J, Kunkel SL
    Am. J. Pathol., 2006-06-01;168(6):1940-50.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development
  17. IL-33, an interleukin-1-like cytokine that signals via the IL-1 receptor-related protein ST2 and induces T helper type 2-associated cytokines.
    Authors: Schmitz J, Owyang A, Oldham E, Song Y, Murphy E, McClanahan TK, Zurawski G, Moshrefi M, Qin J, Li X, Gorman DM, Bazan JF, Kastelein RA
    Immunity, 2005-11-01;23(5):479-90.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Development
  18. Persistence of bronchopulmonary hyper-reactivity and eosinophilic lung inflammation after anti-IL-5 or -IL-13 treatment in allergic BALB/c and IL-4Ralpha knockout mice.
    Authors: Proust B, Nahori MA, Ruffie C, Lefort J, Vargaftig BB
    Clin. Exp. Allergy, 2003-01-01;33(1):119-31.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  19. Murine model of chronic L. (Viannia) panamensis infection: Role of IL-13 in disease
    Authors: Tiago M. Castilho, Karen Goldsmith-Pestana, Caterin Lozano, Liliana Valderrama, Nancy G. Saravia, Diane McMahon-Pratt
    European Journal of Immunology
  20. An IL-9–pulmonary macrophage axis defines the allergic lung inflammatory environment
    Authors: Yongyao Fu, Jocelyn Wang, Baohua Zhou, Abigail Pajulas, Hongyu Gao, Baskar Ramdas et al.
    Science Immunology
  21. Follicular regulatory T cells control humoral and allergic immunity by restraining early B cell responses
    Authors: Rachel L. Clement, Joe Daccache, Mostafa T. Mohammed, Alos Diallo, Bruce R. Blazar, Vijay K. Kuchroo et al.
    Nature Immunology
  22. Contribution of IL-13 to early exocrinopathy in Id3−/− mice
    Authors: Josh Mahlios, Yuan Zhuang
    Molecular Immunology
  23. A protective role for IL-13 receptor alpha 1 in bleomycin-induced pulmonary injury and repair
    Authors: D Karo-Atar, A Bordowitz, O Wand, M Pasmanik-Chor, I E Fernandez, M Itan et al.
    Mucosal Immunology

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Mouse IL-13 Antibody
By Anonymous on 09/05/2021
Application: WB Sample Tested: TF-1 human erythroleukemic cell line Species: Human
Western Blot Mouse IL-13 Antibody MAB413
Mouse IL-13 Antibody
By Leslie Priddy on 04/01/2018
Application: ELISA Sample Tested: Tumor cell lyastes Species: Mouse