Home / IL-12/IL-35 p35 / Mouse IL-12/IL-35 p35 Antibody

Mouse IL-12/IL-35 p35 Antibody

Catalog #: MAB6688
6 Citations Datasheet
Catalog # Availability Size / Price Qty
MAB6688
MAB6688-SP
IL‑12/IL-35 p35 in Mouse Spleen.
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Citations (6)
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Mouse IL-12/IL-35 p35 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-12/IL-35 p35 in direct ELISAs. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse (rm) IL-12 p35+p40 heterodimer or recombinant rat IL-12 p35 is observed. No cross-reactivity with recombinant human IL-12 p35, rmIL-12 p40, rmIL-23 p19+p40 heterodimer, recombinant canine IL-12 p35+p40 heterodimer, or recombinant feline IL-12 p35+p40 heterodimer is observed.
Source
Monoclonal Rat IgG2A Clone # 45806
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived mouse IL-12/IL-35 p35
Arg23-Ala215
Accession # NP_032377
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry IL-12/IL-35 p35 antibody in Mouse Spleen by Immunohistochemistry (IHC-Fr). View Larger

IL‑12/IL-35 p35 in Mouse Spleen. IL-12/IL-35 p35 was detected in immersion fixed frozen sections of mouse spleen using Rat Anti-Mouse IL-12/IL-35 p35 Monoclonal Antibody (Catalog # MAB6688) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hemotoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-12/IL-35 p35

Interleukin 12, also known as Natural Killer Cell Stimulatory Factor (NKSF) or Cytotoxic Lymphocyte Maturation Factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. IL‑12 is produced by macrophages and B lymphocytes and has been shown to have multiple effects on T cells and Natural Killer (NK) cells. Some of these IL‑12 activities include the induction of IFN‑ gamma and TNF in resting and activated T and NK cells; the enhancement of cytotoxic activity of resting NK and T cells, the stimulation of resting T cell proliferation in the presence of a comitogen; and the enhancement of NK cell proliferation. IL‑12 is a key mediator of cellular-immunity and induces the differentiation of Th1 cells from precursor T helper cells. Based on its activities, it has been suggested that IL‑12 may have therapeutic potential as a vaccine adjuvant that promotes cellular-immunity and as an anti-tumor and anti-viral agent. Human and mouse IL‑12 share 70% and 60% amino acid sequence identity in their p40 and p35 subunits, respectively.

Long Name
Interleukin 12 p35
Entrez Gene IDs
3592 (Human); 16159 (Mouse); 84405 (Rat)
Alternate Names
CLMF p35; CLMF; cytotoxic lymphocyte maturation factor 1, p35; Cytotoxic lymphocyte maturation factor 35 kDa subunit; IL-12, subunit p35; IL-12/IL-35 p35; IL-12A; IL35 subunit; IL35p35; interleukin 12, p35; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35); interleukin-12 alpha chain; interleukin-12 subunit alpha; NF cell stimulatory factor chain 1; NFSK; NK cell stimulatory factor chain 1; NKSF1; p35

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Citations for Mouse IL-12/IL-35 p35 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. IL35 attenuated LPS-induced acute lung injury by regulating macrophage polarization
    Authors: Shengsong Chen, Jingen Xia, Yi Zhang, Qingyuan Zhan
    Molecular Biology Reports
  2. IL35 modulation altered survival, cytokine environment and histopathological consequences during malaria infection in mice
    Authors: RO Bello, MA Abdullah, R Abd Majid, VK Chin, MF Abd Rachma, ZO Ibraheem, MK Hussain, MG Magaji, R Basir
    Malar. J., 2019-12-19;18(1):434.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  3. Interleukin-12p35 Knock Out Aggravates Doxorubicin-Induced Cardiac Injury and Dysfunction by Aggravating the Inflammatory Response, Oxidative Stress, Apoptosis and Autophagy in Mice
    Authors: J Ye, Y Huang, B Que, C Chang, W Liu, H Hu, L Liu, Y Shi, Y Wang, M Wang, T Zeng, W Zhen, Y Xu, L Shi, J Liu, H Jiang, D Ye, Y Lin, J Wan, Q Ji
    EBioMedicine, 2018-09-15;35(0):29-39.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  4. Lentiviral delivery of novel fusion protein IL12/FasTI for cancer immune/gene therapy
    Authors: X Yang, X Yu, Y Wei
    PLoS ONE, 2018-07-25;13(7):e0201100.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. Prevention of autoimmune diabetes by ectopic pancreatic beta-cell expression of interleukin-35.
    Authors: Bettini M, Castellaw AH, Lennon GP, Burton AR, Vignali DA
    Diabetes, 2012-03-16;61(6):1519-26.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. IL-18 binding protein (IL-18BP) as a novel radiation countermeasure after radiation exposure in mice
    Authors: X Li, W Cui, L Hull, L Wang, T Yu, M Xiao
    Sci Rep, 2020-10-29;10(1):18674.

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