Mouse IL-12/IL-35 p35 Alexa Fluor® 405-conjugated Antibody

Catalog #: FAB6688V Datasheet

Catalog #	Availability	Size / Price	Qty
FAB6688V-100UG

All IL-12/IL-35 p35 Antibodies

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Mouse IL-12/IL-35 p35 Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IL-12/IL-35 p35 in direct ELISAs. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse (rm) IL-12 p35+p40 heterodimer or recombinant rat IL-12 p35 is observed. No cross-reactivity with recombinant human IL-12 p35, rmIL

Source

Monoclonal Rat IgG2a Clone # 45806

Purification

Protein A or G purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived mouse IL-12/IL-35 p35
Arg23-Ala215
Accession # NP_032377

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration

Sample

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: IL-12/IL-35 p35

Interleukin 12, also known as Natural Killer Cell Stimulatory Factor (NKSF) or Cytotoxic Lymphocyte Maturation Factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. IL‑12 is produced by macrophages and B lymphocytes and has been shown to have multiple effects on T cells and Natural Killer (NK) cells. Some of these IL‑12 activities include the induction of IFN‑ gamma and TNF in resting and activated T and NK cells; the enhancement of cytotoxic activity of resting NK and T cells, the stimulation of resting T cell proliferation in the presence of a comitogen; and the enhancement of NK cell proliferation. IL‑12 is a key mediator of cellular-immunity and induces the differentiation of Th1 cells from precursor T helper cells. Based on its activities, it has been suggested that IL‑12 may have therapeutic potential as a vaccine adjuvant that promotes cellular-immunity and as an anti-tumor and anti-viral agent. Human and mouse IL‑12 share 70% and 60% amino acid sequence identity in their p40 and p35 subunits, respectively.

Long Name

Interleukin 12 p35

Entrez Gene IDs

3592 (Human); 16159 (Mouse); 84405 (Rat)

Alternate Names

CLMF p35; CLMF; cytotoxic lymphocyte maturation factor 1, p35; Cytotoxic lymphocyte maturation factor 35 kDa subunit; IL-12, subunit p35; IL-12/IL-35 p35; IL-12A; IL35 subunit; IL35p35; interleukin 12, p35; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35); interleukin-12 alpha chain; interleukin-12 subunit alpha; NF cell stimulatory factor chain 1; NFSK; NK cell stimulatory factor chain 1; NKSF1; p35