Mouse IL-1 RI Antibody Summary
Leu20-Lys338
Accession # P13504
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of IL‑1 RI in RAW264 cells by Flow Cytometry. RAW264 cells were stained with Rat Anti-Mouse IL‑1 RI Monoclonal Antibody (Catalog # MAB7712, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-1 RI
The type I IL-1 receptor (IL-1 RI, designated IL-1 R1 and CD121a) is one of at least nine members of the IL-1 R family within the Toll/IL-1 R (TIR) superfamily. IL‑1 RI is an ~80 kDa type I transmembrane (TM) protein that binds the pleiotropic cytokines IL-1 alpha and IL-1 beta, plus the IL-1 receptor antagonist (IL-1 Ra). Signal transduction requires complex formation with the IL-1 R accessory protein (IL-1 R AcP/IL-1 R3), another type I TM protein. This complex recruits the adaptor protein MyD88 to initiate signaling in the NF kappa B pathway. Mouse IL-1 RI cDNA encodes a 576 amino acid (aa) protein that contains a 19 aa signal sequence, a 319 aa extracellular domain (ECD) with three C2-type Ig-like domains, a 21 aa TM domain and a 217 aa cytoplasmic region with a TIR domain. Mouse IL-1 RI shares 64%, 83%, 60%, 61% and 55% aa identity with human, rat, canine, equine and bovine IL-1 RI, respectively. The role of IL-1 in inflammation is under several levels of control, including expression and activation of IL-1 alpha and IL-1 beta, expression of IL-1 RI and its accessory and adaptor proteins, and negative regulators such as the IL‑receptor family member, IL-1RII/IL-1R2. IL-1 RI is expressed predominantly by T cells, fibroblasts, and endothelial cells and mediates acute phase inflammatory responses including fever.
Product Datasheets
Citation for Mouse IL-1 RI Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Multiple CD11c+ cells collaboratively express IL-1beta to modulate stromal vascular endothelial growth factor and lymph node vascular-stromal growth.
Authors: Benahmed F, Chyou S, Dasoveanu D, Chen J, Kumar V, Iwakura Y, Lu T
J Immunol, 2014-03-21;192(9):4153-63.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
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