Mouse IgG2B Isotype Control

Catalog #: MAB0042
9 Citations 1 Review Datasheet

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MAB0042

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Citations (9)

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Reviews (1)

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Mouse IgG2B Isotype Control Summary

Specificity

Recognizes the E-tag peptide. No background staining was detected by immunohistochemistry on a variety of tissues.

Source

Monoclonal Mouse IgG_2B Clone # 73009

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant protein with a C-terminal E-tag (aa sequence GAPVPYPDPLEPR)

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Control

Negative control for immunohistochemistry or immunocytochemistry experiments. Use at the same concentration as the detection antibody.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IgG2B

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G2B Fc

Alternate Names

IgG2B

Product Datasheets

Product Datasheet

Citations for Mouse IgG2B Isotype Control

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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Humanized anti-DEspR IgG4S228P antibody increases overall survival in a pancreatic cancer stem cell-xenograft peritoneal carcinomatosis ratnu/nu model
Authors: CM Gromisch, GLA Tan, KA Pasion, AM Moran, MS Gromisch, MW Grinstaff, FJ Carr, VLM Herrera, N Ruiz-Opazo
BMC Cancer, 2021-04-14;21(1):407.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Suppression of glycogen synthesis as a treatment for Lafora disease: Establishing the window of opportunity.
Authors: Olga Varea, Jordi Duran, Mònica Aguilera, Neus Prats, Joan J. Guinovart
Neurobiology of Disease
In situ characterization of intrahepatic non-parenchymal cells in PSC reveals phenotypic patterns associated with disease severity.
Authors: Berglin L, Bergquist A, Johansson H, Glaumann H, Jorns C, Lunemann S, Wedemeyer H, Ellis E, Bjorkstrom N
PLoS ONE, 2014-08-20;9(8):e105375.
Species: Human
Sample Types: Whole Tissue
Applications: Isotype Control
Seminal plasma did not influence the presence of transforming growth factor-beta 1, interleukine-10 and interleukin-6 in porcine follicles shortly after insemination
Authors: Jiwakanon Jatesada, Persson Elisabeth, Dalin Anne-Marie
Acta Veterinaria Scandinavica
Brain-derived neurotrophic factor is increased in serum and skin levels of patients with chronic spontaneous urticaria.
Authors: Rossing K, Novak N, Mommert S, Pfab F, Gehring M, Wedi B, Kapp A, Raap U
Clin. Exp. Allergy, 2011-06-16;41(10):1392-1399.
Species: Human
Sample Types: Whole Tissue
Applications: Control
S100A11, an dual mediator for growth regulation of human keratinocytes.
Authors: Sakaguchi M, Sonegawa H, Murata H, Kitazoe M, Futami J, Kataoka K, Yamada H, Huh NH
Mol. Biol. Cell, 2007-10-31;19(1):78-85.
Species: Human
Sample Types: Whole Cells
Applications: Control
Developmental differences in the responses of IL-6 and IL-13 transgenic mice exposed to hyperoxia.
Authors: Choo-Wing R, Nedrelow JH, Homer RJ, Elias JA, Bhandari V
Am. J. Physiol. Lung Cell Mol. Physiol., 2007-03-30;293(1):L142-50.
Species: Mouse
Sample Types: Whole Tissue
Applications: Control
Hepatitis C virus (HCV)-specific CD8+ cells produce transforming growth factor beta that can suppress HCV-specific T-cell responses.
Authors: Alatrakchi N, Graham CS, van der Vliet HJ, Sherman KE, Exley MA, Koziel MJ
J. Virol., 2007-03-21;81(11):5882-92.
Species: Human
Sample Types: Whole Cells
Applications: Control
Susceptibility of human testis to human immunodeficiency virus-1 infection in situ and in vitro.
Authors: Roulet V, Satie AP, Ruffault A, Le Tortorec A, Denis H, Guist'hau O, Patard JJ, Rioux-Leclerq N, Gicquel J, Jegou B, Dejucq-Rainsford N
Am. J. Pathol., 2006-12-01;169(6):2094-103.
Species: Human
Sample Types: Whole Cells
Applications: Control